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Wheat blumeria graminis culture and preparation method for test

A technology of wheat powdery mildew and wheat powdery mildew, which is applied in the field of cultivation and preparation of wheat powdery mildew for testing, can solve the problems of bacteria culture and preparation, difficulties in collection and preservation, and inability to grow, and achieve the effect of large amount of bacteria and good stability

Pending Publication Date: 2018-12-11
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because wheat powdery mildew is an obligate parasite, it needs to obtain the necessary nutrients from the living host tissue, and the bacteria cannot grow on the culture medium. There are still great difficulties in the cultivation, preparation, collection and preservation of pathogenic bacteria

Method used

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  • Wheat blumeria graminis culture and preparation method for test
  • Wheat blumeria graminis culture and preparation method for test

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: Isolation, purification and propagation of wheat powdery mildew bacteria

[0036] (1) In the ultra-clean workbench, take the high-sensitivity wheat Jinfeng No. 1 and plant it in a large glass tube for 10 days, so that the wheat seedlings are in the "one leaf, one heart" stage to obtain sterile tube seedlings;

[0037] (2) The wheat leaves collected in the field carrying powdery mildew were transferred to the wheat seedlings growing in a large glass tube by shaking off the spores, and the glass tube was covered with 4 layers of gauze to isolate miscellaneous bacteria. , Grow for 5 days under the conditions of 12000-18000LX light and 50-60% humidity;

[0038] (3) When the infection point can be seen on the wheat leaf surface but the conidia have not yet grown, cut off the leaf segment containing a single conidium pile, and culture it on 0.45% water agar containing 60 mg / L benzimidazole cultured in a light incubator with a temperature of 17°C, a light of 120...

Embodiment 2

[0042] Embodiment 2: collect wheat powdery mildew bacteria

[0043] (1) Planting potted seedlings: Take a plastic pot with a diameter of 11.5cm and a height of 10cm, compact the nutrient soil in the pot, place Jinfeng No. 1 seeds (as close as possible to the center of the pot), and then cover the seeds with thin soil. Doing so can reduce the floating soil in the later stage, and the wheat seedlings are more concentrated, which is conducive to the collection of bacteria.

[0044] (2) When the wheat seedlings just grow, turn the pot seedlings upside down, and use tweezers to gently flick off the layer of floating soil covering the wheat seedlings, so as to prevent the soil on the pot surface from falling down and affecting the collection of bacteria.

[0045] (3) if figure 1 Shown, with diameter 9.5cm after emergence, thickness 2.0cm, high 29.5cm upper end is covered with the glass tube cover of 5 layers of gauze, prevents seedling from being infected.

[0046] (4) When the p...

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Abstract

The invention discloses a wheat blumeria graminis culture and preparation method for test, and aims to solve the technical problems that culture and collection for a great number of wheat blumeria graminis for test are difficult. The wheat blumeria graminis culture and preparation method for test comprises the following steps: (1) separating and purifying a wheat blumeria graminis strain; (2) expanding and propagating the wheat blumeria graminis strain; and (3) collecting the wheat blumeria graminis. The method adopts a living way, the wheat blumeria graminis can continuously grow on a wheat seedling in a colonizing mode, so that the strain has growth advantages; and manual seedling-shearing in-vitro culture is not needed for collecting the strain, so that the method is simple and convenient, is time-saving and is labor-saving; and the collected wheat blumeria graminis is great in number.

Description

technical field [0001] The invention relates to the technical field of plant pathogenic parasites, in particular to a method for cultivating and preparing wheat powdery mildew bacteria for testing. Background technique [0002] by powdery mildew brucelli ( Blumeria graminis(DC.) E.O.Speer f.sp.tritici ) Infection caused by wheat powdery mildew occurs in almost all wheat production areas around the world, especially after the 1980s, wheat powdery mildew has been popular for many times and has become an important disease that seriously restricts wheat production. Wheat powdery mildew mainly occurs on leaves, and can also occur on plant leaf sheaths, stems and ears. Generally, there are more disease spots on the front of the leaves than on the back of the leaves, and the lower leaves are more severely affected than the upper leaves. There is a layer of white powdery mildew on the surface of the diseased part. White filamentous mildew spots first appear on the diseased part, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12N3/00C12R1/645
CPCC12N1/02C12N1/14C12N3/00
Inventor 李亚红王俊美徐飞韩自行宋玉立刘露露李丽娟张姣姣
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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