Outdoor totally closed high-density phaeodactylum tricornutum bohlin culture method

A high-density culture and fully enclosed technology, applied in the field of microalgae cultivation, can solve the problems of outdoor cultivation of Phaeodactylum tricornutum, lack of mention of Phaeodactylum tricornutum, low controllability, etc., and facilitate large-scale production Production, resistance to outdoor uncertainties, and strong controllability

Inactive Publication Date: 2018-07-03
云南爱尔发生物技术股份有限公司
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  • Claims
  • Application Information

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Problems solved by technology

[0004] Patent CN1710060A discloses a kind of open culture method of Phaeodactylum tricornutum and its special culture medium, described as preparing culture medium (ZBPT) with seawater, inoculating by the volume ratio of algae liquid: culture medium 1:9, in 19 ~ 25 ℃, light intensity 3000 ~ 6000Lux, light time 8 ~ 12h / d under the conditions of aeration culture, this method can effectively prevent and control green algae pollution, and can cultivate Phaeodactylum tricornutum on a large scale in an open environment, but compared to outdoor Fully enclosed large-scale cultivation of Phaeodactylum tricornutum still has disadvantages such as low controllability, low efficiency, and large pollution
[0005] The patent CN103834570A discloses a culture method and culture medium for the mixed culture of Phaeodactylum tricornutum and Nitzschia minor, which is to increase the content of fatty acids in the mixed culture of the two in a special medium, compared to the traditional use of f / 2 The culture medium and the method of developing separate cultivation in cement ponds have the characteristics of strong controllability, fast growth, high yield, and low pollution, but there is no mention of outdoor large-scale fully enclosed cultivation of Phaeodactylum tricornutum
[0006] Patent CN1059631A discloses a salt algae culture method and a culture pond, which use brine or inland lake salt to prepare brine or intercrystalline brine, underground brine, etc. It is suitable for outdoor algae cultivation in coastal and inland salt-producing areas and in bad weather such as rain, wind, and hail, but it fails to mention the outdoor cultivation of Phaeodactylum tricornutum

Method used

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  • Outdoor totally closed high-density phaeodactylum tricornutum bohlin culture method
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  • Outdoor totally closed high-density phaeodactylum tricornutum bohlin culture method

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Experimental program
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Effect test

Embodiment 1

[0050] Phaeodactylum tricornutum was cultivated in an outdoor fully enclosed independent pipe reactor:

[0051] 1. Preparation of A100 medium

[0052] 1) Put 1 ton of water filtered by the membrane module into the culture medium configuration tank, add 120ppm sodium hypochlorite solution for circulation disinfection for 24 hours, then add sodium thiosulfate for neutralization, after testing that there is no residual chloride ion, add nutrients for culture medium preparation. 2), Quantitatively weigh the following substances and add them to the configuration pool for medium preparation: sodium chloride (NaCl) 27.7g / L, magnesium chloride (MgCl) 2 )5.20g / L, magnesium sulfate (MgSo 4 ) 7.13g / L, potassium chloride (KCl) 0.745g / L, calcium chloride (CaCl 2 ) 1.14g / L, sodium bromide (NaBr) 0.837g / L, sodium bicarbonate (NaHCO 3 ) 0.210g / L; Sodium nitrate (NaNO 3 )600mg / L, sodium dihydrogen phosphate (NaH 2 po 4 .2H 2 o) 110mg / L, sodium silicate (Na 2 SiO 3 .9H 2 o) 100 mg / L....

Embodiment 2

[0058] Phaeodactylum tricornutum was cultivated in an outdoor non-fully enclosed independent pipe reactor:

[0059] 1, the preparation of culture medium is the same as embodiment 1;

[0060] 2. Phaeodactylum tricornutum was cultivated in an outdoor non-fully enclosed independent pipeline reactor

[0061] According to the ratio of Phaeodactylum tricornutum to A100 medium volume ratio of 1:1, it was inoculated into the open track pool for cultivation, and the conditions were: temperature: 20-25°C; light: 1000-80000Lux; PH: 7.5-8.5; L / min air, 2.5L / min carbon dioxide aeration circulation culture.

[0062] The growth curve of Phaeodactylum tricornutum in non-closed outdoor high-density culture is as follows: figure 2 As shown, the culture results show that after dilution with medium, the theoretical inoculation density OD (680) drops from 1.5 to 2.0 and the density of Phaeodactylum tricornutum increases from 0.9 to 0.95. The entire growth period is 8 days, and the density canno...

Embodiment 3

[0064] Continuous cultivation of Phaeodactylum tricornutum in an outdoor fully enclosed independent pipe reactor:

[0065] 1, the preparation of culture medium is the same as embodiment 1;

[0066] 2. Continuous cultivation of Phaeodactylum tricornutum in an outdoor fully enclosed independent pipeline reactor

[0067] According to the ratio of Phaeodactylum tricornutum to A100 medium volume ratio of 1:1, it was inoculated into an independent pipeline reactor for cultivation, and the conditions were: temperature: 20-25°C; light: 1000-80000Lux; PH: 7.5-8.5; 4.5L / min air, 2.5L / min carbon dioxide aeration circulation culture.

[0068] The growth curve of Phaeodactylum tricornutum fully enclosed outdoor high-density continuous culture is as follows: image 3 As shown, the culture results show that after dilution with medium, the theoretical inoculation density OD (680) decreases from 1.5 to 2.0 and the density of Phaeodactylum tricornutum increases to 5.0 and above. The whole gro...

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Abstract

The invention discloses an outdoor totally closed high-density phaeodactylum tricornutum bohlin culture method. The method comprises the following steps: 1) by taking phaeodactylum tricornutum bohlinsolution with OD(680) of 1.5-2.0 as algae species, inoculating a totally closed glass pipeline, and culturing; 2) cultivating for 5-7 days, and harvesting 50-80% when the OD(680) is 5.0 and above; 3)according to the actual growth condition of algae cells, inoculating phaeodactylum tricornutum bohlin solution, having the same volume of the harvested algae solution in the step 2) and the OD(680) of1.5-2.0, as the algae species or a culture medium, cultivating again, and realizing semi-continuous culture; 4) repeating operations of the steps 2) and 3) for 3-5 times; 5) harvesting completely, cleaning a cultivation pipeline, inoculating the phaeodactylum tricornutum bohlin solution with the OD(680) of 1.5-2.0 as the algae species, and carrying out the next batch production; and 6) repeatingthe steps 1)-5), so that continuous production is realized. The method disclosed by the invention is high in efficiency and easy to operate.

Description

technical field [0001] The invention belongs to the technical field of microalgae cultivation, and in particular relates to a method for cultivating Phaeodactylum tricornutum at high density outdoors. Background technique [0002] Phaeodactylum tricornutum is a typical unicellular microalgae, and it is also a marine economic unicellular microalgae with economic value. Finger algae. Phaeodactylum tricornutum is easy to grow, and it can adapt to a wide range of environments. It can survive normally in the salinity range of 9‰~92‰, and its optimum salinity is 20‰~24‰. The optimum temperature range is 5-30°C, and the optimum temperature is 18-24°C. Even at 0°C, a small amount of Phaeodactylum tricornutum will reproduce, but if it exceeds 35°C, the cells will stop growing and eventually all die. The suitable light intensity range of Phaeodactylum tricornutum is 1000-80000 lux, and its optimum light range is 10000-30000 lux. Phaeodactylum tricornutum has the characteristics of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 郑学军张吉和吴澄杨泽娥王兴勇张勇
Owner 云南爱尔发生物技术股份有限公司
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