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Application of chelidonine for preparing plant inducer and plant inducer

A technology of chelidonine and plant-induced resistance, which is applied in the fields of chemicals for biological control, plant growth regulators, applications, etc., can solve the problem of no relevant research reports on agricultural activity, and achieve a significant effect of inducing disease resistance Effect

Active Publication Date: 2021-08-17
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of agricultural use, it has been reported in the literature that chelidonine has a certain insecticidal effect on gypsy moth larvae and red pine caterpillars. There are no relevant research reports on other agricultural activities, and its application in agriculture remains to be further developed.

Method used

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  • Application of chelidonine for preparing plant inducer and plant inducer
  • Application of chelidonine for preparing plant inducer and plant inducer
  • Application of chelidonine for preparing plant inducer and plant inducer

Examples

Experimental program
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Effect test

preparation example Construction

[0062] The preparation method of above-mentioned soluble powder is:

[0063] Mix chelidonine, stabilizer, filler, dispersant and surfactant in proportion, dissolve in 0.01mol / L hydrochloric acid solution at room temperature, and stir until fully dissolved. Dry it at 50-70°C for 5-10 days with a box dryer to obtain a solid. The solid is pulverized, (the non-water-soluble filler does not participate in the previous step, and is directly mixed with the solid) and passed through a 320-mesh sieve to obtain the chelidonine soluble powder.

[0064] The preparation method of above-mentioned water-dispersible granule is:

[0065] The chelidonine, filler, dispersant and surfactant are mixed in a certain proportion, and pulverized by a pulverizer. Add water and stir evenly before granulating. After drying with a dryer at 50-70°C, sieve to obtain granules with a particle size of 2-4mm, which are chelidonine water-dispersible granules.

[0066] The preparation method of above-mentioned...

Embodiment 1

[0073] Embodiment 1: Determination of the direct anti-TMV activity of chelidonine

[0074] (1) Protective activity

[0075] Prepare 0.1mg / mL and 0.1mg / mL chelidonine solutions, select healthy 5-6-leaf stage tobacco with consistent growth, and inoculate 2000-fold diluted TMV solution 48 hours after spraying the drug, and the blank control is treated with water. The positive control is 500-fold solution of Altaline (6% oligosaccharide·catenin). Each treatment was inoculated with 3 leaves and repeated 3 times. After 3 days, the number of dead spots was counted to calculate the inhibition rate.

[0076] (2) in vitro passivation activity

[0077] Prepare 0.1mg / mL and 0.4mg / mL chelidonine solutions and mix them with equal volumes of 1000-fold diluted TMV solution. Place it at room temperature for 1 hour and inoculate it in the same-growing, healthy 5-6-leaf stage tobacco leaves. The blank control was treated with clear water, and the positive control was Alterin solution. Each ...

Embodiment 2

[0086] Embodiment 2: Determination of chelidonine-induced tobacco anti-TMV activity

[0087] Two varieties of heart leaf tobacco and common tobacco (K326) were selected to verify the induced disease resistance activity. Heart leaf tobacco can form virus scabs, while the symptom on ordinary tobacco is mosaic. Different symptoms use different methods to count the disease resistance. Spray chelidonine at 0.1 and 0.1 mg / mL to the lower three leaves of tobacco at the 6-7 leaf stage with consistent growth. After 48 hours, TMV was inoculated to the unsprayed upper leaves. The blank control was treated with clear water, and the positive control was Alterin solution. Each plant was inoculated with 2-3 leaves, each treatment included 10 tobacco plants, and the whole experiment was repeated 3 times. After 3 days, the number of dead spots and the disease index of common tobacco were counted. The formula for calculating the inhibition rate was as above, and the formula for calculating th...

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Abstract

The invention discloses the application of chelidonine for preparing a plant resist and the plant resist. In the present invention, the disease-inducing activity of chelidonine is clarified by measuring the biological activity in vitro and in vivo, and the disease-inducing activity of chelidonine is determined by measuring the changes of physiological indexes and disease-resistance-related genes in tobacco caused by chelidonine. mechanism. The preparation method of chelidonine inducer is to prepare chelidanine into soluble powder, water dispersible granule, soluble liquid, water emulsion and microemulsion through a certain processing process, which are used in tobacco, kiwi fruit, strawberry, Control of Tobacco mosaic virus (TMV), kiwifruit canker (Pseudomonas syringae PV actinidia), strawberry gray mold (Botrytis cinerea), cucumber downy mildew (Pseudoperonospora cubensis) and wheat flour (Blumeria graminis) on cucumber, wheat and other crops ) and other plant fungal, oomycete, bacterial and viral diseases. The preparation contains 0.1% to 5% of chelidrine, and the rest are auxiliary agents.

Description

technical field [0001] The invention belongs to the field of plants and applications thereof, and in particular relates to the application of chelidonine for preparing plant inducers and the plant inducers. Background technique [0002] Natural products are substances derived from a single chemical component or multiple chemical components in all organisms in nature. Sources mainly include plants, animals, microorganisms and marine organisms. The use of traditional "Chinese herbal medicine" in my country shows that natural products of plant origin have been used as medicines for thousands of years. In terms of agricultural use, there were records of using plants to control agricultural pests in the 7th to 5th centuries BC. Natural products have diverse and novel chemical structures, unique biological activity mechanisms, good environmental compatibility, and slow development of drug resistance. They are important resources for the development of medicines and pesticides. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01N25/04A01P3/00A01P1/00
CPCA01N25/04A01N43/90
Inventor 冯俊涛郭文慧闫合何军王勇
Owner NORTHWEST A & F UNIV
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