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Bacillus for preventing wheat diseases, as well as preparation and use thereof

A technology for bacillus and wheat take-all disease, applied in botany equipment and methods, microbe-based methods, biochemical equipment and methods, etc., can solve the problem of little research on bacillus, and achieve strong inhibition of the main pathogenic fungi of wheat Active, non-toxic and side effects, easy to operate

Inactive Publication Date: 2009-07-22
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many studies on Bacillus, but there are very few studies on endophytic Bacillus

Method used

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  • Bacillus for preventing wheat diseases, as well as preparation and use thereof
  • Bacillus for preventing wheat diseases, as well as preparation and use thereof
  • Bacillus for preventing wheat diseases, as well as preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: Isolation and identification of E1R-j bacterial strain

[0036]Rinse the naturally growing wheat root system collected from Changwu County, Shaanxi Province with tap water and spread it out to dry. Take 1 / 4 of the root system from each plant and cut it into pieces. Mix the root samples of all plants and weigh 1g for surface disinfection (70% alcohol for 60s→ 3. Treat in 125% NaClO for 6min → 70% alcohol for 30s → rinse with sterile water 3-5 times). Take 200 μL of the sterile water rinsed for the last time and spread it on the PDA plate to test whether the surface disinfection is thorough. Grind the surface-sterilized sample material with 9 mL of sterile water in a sterilized mortar until it becomes a paste, take the original solution, diluted 100-fold solution and 1000-fold solution, shake well, and apply 200 μL on the mixture with carbendazim (50 μg / mL, 25% wettable powder, produced by Zhejiang Yifan Agrochemical Factory) on the PDA plate, each treatme...

Embodiment 2

[0039] Embodiment 2: the preparation method of E1R-j bacterial strain thalline

[0040] Inoculate the E1R-j strain stored at -80°C on NA medium, culture it at 25-28°C for 2 days, inoculate its single colony into 100mL LB (Luria-Bertani: 10g peptone; 10g yeast powder; 5g NaCl; 1000mL distilled water , adjust pH=7.2) in the culture solution, shake and cultivate in the dark at 37°C and 150rpm for 18-24h, then inoculate 1% seed solution into 100mL of LB culture solution, and cultivate in the dark at 28°C and 150rpm for 48h, the obtained bacteria After the solution was centrifuged at 8000rpm for 20min, the precipitate obtained was the bacterial cell of the E1R-j strain.

Embodiment 3

[0041] Embodiment 3: the preparation method of the antibacterial active substance of E1R-j bacterial strain

[0042] Inoculate the E1R-j strain stored at -80°C on NA medium, and after culturing at 25-28°C for 2 days, inoculate its single colony into 100mL LB culture medium, culture at 37°C and 150rpm in the dark for 18-24h, then Inoculate 1% of the seed liquid into 100mL of LB culture liquid, culture at 28°C and 150rpm in the dark for 40-48h with shaking, and centrifuge the obtained bacterial liquid at 8000rpm for 20min. Salt out, overnight at 4°C, centrifuge at 12000rpm for 30min to dialyze and concentrate the precipitate.

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PUM

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Abstract

The invention discloses a Bacillus sp. E1R-j, which is preserved in China General Microbiological Culture Center of Microbial Culture Collection Management Committee on August 16th, 2007 with the preservation number of CGMCC No.2134; the invention further discloses a strain separating and identifying method, a thallus preparation method and a preparation method of an antimicrobial active substance, as well as applications of the E1R-j strain, the thallus and the antimicrobial active substance of the strain in controlling diseases including wheat take-all, Blumeria graminis and wheat yellow stripe rust as well as promoting seed germination and growth of wheat. The E1R-j strain is obtained from wheat root, has simple screening method, convenient operation, stable product quality, environmental protection and remarkable effects on controlling wheat take-all, Blumeria graminis and wheat yellow stripe rust, and does not have toxic side effect.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and relates to a new bacterial strain for preventing and treating wheat diseases, in particular to a bacillus for preventing and controlling wheat diseases, a preparation method and application thereof. Background technique [0002] Wheat is an important food crop worldwide, and about 35% to 40% of the world's population uses wheat as their main food (Jin Shanbao. Chinese Wheat Science. Beijing: China Agricultural Press, 1996: 1-3.). Since the 1980s, with the promotion of dwarf and semi-dwarf varieties and the improvement of water and fertilizer conditions, the occurrence and damage of wheat diseases have become increasingly serious in my country. Wheat stripe rust has been a disaster in the southwestern and northwestern wheat regions of my country from 2001 to 2005. It has developed from an occasional disease to a frequent disease, which makes the wheat production in the vast wheat...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P21/00A01N63/02A01P3/00A01P21/00C12R1/07
Inventor 黄丽丽刘冰乔宏萍康振生宫宇飞高小宁
Owner NORTHWEST A & F UNIV
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