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Bacillus for preventing wheat take-all and preparation thereof

A technology of wheat take-all disease and Bacillus, applied in the field of biopesticides, can solve the problems that microorganisms are easily affected by external conditions, little research on Bacillus, and unstable control effects, etc., and achieve strong anti-take-all bacteria activity, products Stable quality and remarkable effect

Inactive Publication Date: 2009-07-22
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many studies on the biological control of wheat take-all, and commercial preparations have been used in production, but there are still some problems to be solved: (1) For a long time, soil and rhizosphere microorganisms are the most effective bacteria for the control of wheat take-all. The main source, but these microorganisms are easily affected by external conditions, are not easy to colonize in plants, and the control effect is unstable, etc.
At present, there are many studies on Bacillus, but there are very few studies on endophytic Bacillus

Method used

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  • Bacillus for preventing wheat take-all and preparation thereof
  • Bacillus for preventing wheat take-all and preparation thereof
  • Bacillus for preventing wheat take-all and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Isolation and identification of EDR4 bacterial strain

[0035] Wheat roots are collected from naturally growing wheat fields in Changwu County, Shaanxi Province. Rinse them with tap water and spread them out to dry. Take 1 / 4 of the roots of each plant and cut them into pieces. Weigh 1g of root samples from all plants for surface disinfection (70% alcohol for 60s→ 3. Treat in 125% NaClO for 6 minutes → 70% alcohol for 30 seconds → rinse with sterile water 3 to 5 times). Take 200 μL of the sterile water rinsed for the last time and spread it on the PDA plate to test whether the surface disinfection is thorough. Grind the surface-sterilized sample material with 9 mL of sterile water in a sterilized mortar until it becomes a paste, take the original solution, diluted 100-fold solution and 1000-fold solution, shake well, and apply 200 μL on the mixture with carbendazim (50 μg / mL, 25% wettable powder, produced by Zhejiang Yifan Agrochemical Factory), each trea...

Embodiment 2

[0038] Embodiment 2: the preparation method of EDR4 bacterial strain thalline

[0039] Inoculate the EDR4 strain preserved at -80°C on NA medium, and after culturing at 25-28°C for 2 days, pick a single colony into 100mL LB culture medium (Luria-Bertani: 10g peptone; 10g yeast powder; 5g NaCl; 1000mL distilled water , adjusted to pH=7.2), cultured at 37°C and 150rpm in the dark for 18-24h with shaking, then inoculated 1% of the seed solution into 100mL of LB culture solution, and cultivated at 28°C and 150rpm in the dark for 48h, and the obtained bacterial liquid was passed through After centrifugation at 8000rpm for 20min, the obtained precipitate is the bacterial cell of the EDR4 strain.

Embodiment 3

[0040] Embodiment 3: the preparation method of the antibacterial active substance of EDR4 bacterial strain

[0041] Inoculate the EDR4 strain preserved at -80°C on NA medium, and after culturing at 25-28°C for two days, pick a single colony into 100mL LB culture medium (Luria-Bertani: 10g peptone; 10g yeast powder; 5g NaCl; 1000mL distilled water , adjusted to pH=7.2), cultured at 37°C and 150rpm in the dark for 18-24h with shaking, then inoculated 1% of the seed solution into 100mL of LB culture solution, and cultivated at 28°C and 150rpm in the dark for 48h, and the obtained bacterial liquid was passed through After centrifugation at 8000rpm for 20min, the resulting sterile supernatant was salted out with 80% saturated ammonium sulfate, overnight at 4°C, and the precipitate obtained by centrifugation at 12000rpm for 30min was dialyzed and concentrated to obtain the antibacterial active substance.

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Abstract

The invention discloses a Bacillus sp. EDR4 strain, which is preserved in China General Microbiological Culture Center of Microbial Culture Collection Management Committee on August 16th, 2007 with the preservation number of CGMCC No.2133; the invention further discloses a separating and identifying method of the strain, a preparation method of thallus and a preparation method of an antimicrobial active substance, as well as applications of the EDR4 strain, the thallus and the antimicrobial active substance of the strain in controlling wheat take-all and promoting wheat growth. The EDR4 strain has stable and natural source of raw materials, simple screening method, convenient operation, stable product quality, environmental protection, remarkable wheat take-all controlling effect and convenient popularization and application, and does not have toxic side effect.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and relates to a new bacterial strain for preventing and treating wheat take-all, in particular to a bacillus and a preparation method thereof for preventing and treating wheat take-all. Background technique [0002] Wheat take-all is a devastating disease infecting the roots of wheat. It is an important disease in major wheat producing areas in the world. It is more serious in the northern winter wheat area and northwest spring wheat area in my country, causing huge losses to agricultural production. Take-all disease of wheat has occurred in Bin County, Xianyang and other places in Shaanxi Province in the 1980s. Since the 1990s, there have been take-all diseased fields and diseased areas in 23 counties and districts in the Weibei Dry Plateau, the lower reaches of the Luohe River, and the northern foot of the Qinling Mountains in the Guanzhong area. trend of expansion. At present, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P21/00A01N63/02A01P3/00A01P21/00C12R1/07
Inventor 黄丽丽刘冰康振生乔红萍
Owner NORTHWEST A & F UNIV
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