Spray vaccination method for Blumeria graminis f.sp.tritici
A technology of wheat powdery mildew, spray inoculation, applied in horticultural methods, botanical equipment and methods, methods based on microorganisms, etc., can solve the problems of uneven powdery mildew bacteria, inability to accurately quantify, etc., and achieve uniform inoculation effect
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Embodiment 1
[0019] Different spore mass concentration inoculation effect of embodiment 1
[0020] Cut the first leaf of the pre-prepared susceptible variety "Jinmai 47" to a length of 3 cm, select 10 leaves face up and put them on the agar fresh-keeping medium containing benzimidazole, and collect them on the ultra-clean bench for pre-propagation. Put fresh conidia that have propagated into a sterile 5mL centrifuge tube, draw 4mL of C5-18-perfluoroalkane (FC-40) solution into the centrifuge tube containing spore powder, and make an inoculum suspension. Use a Vortex shaker to oscillate for 30s to suspend the spores evenly in the solution, draw 10 μL of the suspension and adjust the spore concentration to 10 with a hemocytometer under a microscope 6 、10 5 、10 4 、10 3 and 10 2 Each / mL, as the inoculum for later use; at the same time, use the FC-40 solution without spores as a negative control to observe whether FC-40 damages the wheat leaves. After inoculation, place it in the growth ro...
Embodiment 2
[0022] Embodiment 2, different spray amount inoculation effect
[0023] The preparation of inoculated leaves and inoculum is the same as in Example 1.
[0024] The spray volumes were 3mL, 4mL and 5mL respectively under the Porter spray tower, and placed in the growth room after inoculation (17±1°C, 17-19h light / day) for 8-10 days. The observation results showed that there was no difference in the inoculation effect of different spray volumes, and all of them could fully morbidity.
Embodiment 3
[0025] Embodiment 3, different wheat varieties disease resistance identification effect
[0026] According to the inoculation effect of different spore mass concentrations and the requirements of variety disease resistance identification, the concentration of spores used for disease resistance identification is 1×10 6 individual / ml. Cut the first leaf of 30 wheat varieties prepared in advance and interrupt the length of 3cm, and the blade face is placed on the agar fresh-keeping medium containing benzimidazole, and the inoculum spore suspension is prepared according to the method in Example 1, and the spores are adjusted Concentration 1×10 6 individual / ml. Place the 30 wheat varieties to be tested under the Porter spray tower, take 4mL of the spore suspension for spray inoculation, and continue to place it under the spray tower for 1-2 minutes after inoculation to ensure sufficient settlement of the inoculated spores. After inoculation, place it in the growth room and culti...
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