A seedling stage identification method of cowpea resistance to fusarium wilt

Abstract

The invention belongs to the field of detection of disease resistance of horticultural plants, and relates to a seedling stage identification method for cowpea wilt resistance. The method includes the steps of sprouting cowpea seeds, sowing, cultivating seedlings, separating and cultivating bacterial strains special for inoculation, preparing oxysporum spore suspension liquid, infecting pathogenic bacteria, surveying plant disease causes and the like. Cowpea seedlings are infected with oxysporum artificially with the method that seedling roots are infected with bacterium liquid, cowpea seeds are cultivated in seedling cultivating matrixes to grow two leaves and one bud, then the seedlings are pulled out from infection, the growth vigour and the size of the cowpea seedlings can be controlled easily, the cowpea seedlings can be pulled out easily, and consistency and reliability of identification results are guaranteed. The special indication bacterial strains, the appropriate infection time and the appropriate oxysporum spore suspension liquid concentration are adopted, the infection effect is good, and even inoculation and quick disease generation are achieved. By means of the method, the oxysporum infection only needs to be conducted on different plants with the consistent growth vigour after roots are damaged directly through natural friction, the method is easy and convenient to operate, quick in identification, reliable in result and particularly suitable for identifying the oxysporum disease resistance of cowpea cultivating materials in batches.

Description

technical field [0001] The invention belongs to the technical field of disease resistance identification of horticultural crops, in particular to a seedling stage identification method for cowpea resistance to fusarium wilt, and is related to the technical field of early rapid identification of resistant materials in crop disease resistance breeding. Background technique [0002] Cowpea (scientific name Vignaunguiculata) is commonly known as carob, ginger bean, belt bean, and hanging bean. It belongs to the annual plant of the legume family and is mainly produced in tropical, subtropical and temperate regions. Cowpea has a long history of cultivation in my country, and the planting area is wide. Except for Qinghai and Tibet, it is planted in all provinces and municipalities of the country. In recent years, the planting area of ​​cowpea in my country has remained above 330,000 hectares. Hebei, Henan, Jiangsu, Zhejiang, Anhui, Sichuan, Chongqing, Hubei, Hunan, Guangxi and oth...

AI Technical Summary

Problems solved by technology

Among them, the difference between the germination rate of the radicle method and the growth vigor of the seedlings often leads to inconsistent results, which cannot accurately reflect the resistance at the seedling stage, and the period from radicle inoculation to the emergence of symptoms at the seedling stage is relatively long

Although the root-cutting seedling soaking method has a short cycle and is simple and feasible, the length of the root-cutting is likely to be uneven, and root-cutting is time-consuming and laborious

Both identification methods have certain shortcomings, which is one of the reasons for the significant difference between the identification results at the seedling stage and the identification of artificial disease gardens

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