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Construction method of barley blumeria graminis pathotype differential host system

A technology for powdery mildew bacteria and pathogenic types, which is applied in the establishment and application field of identifying pathogenic types of barley powdery mildew bacteria, and can solve the problems of small differentiation of pathogenic types and poor discrimination

Inactive Publication Date: 2011-07-13
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The object of the present invention is to provide a set of identification system for barley powdery mildew pathogenic type which is still blank in my country, and to directly adopt foreign identification host system to have the defects of small pathogenic type discrimination and poor discrimination. Strong, host system for identifying barley powdery mildew pathogenic types and its construction method that can reasonably and accurately reflect the interaction between barley hosts and barley powdery mildew pathogens in my country

Method used

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  • Construction method of barley blumeria graminis pathotype differential host system
  • Construction method of barley blumeria graminis pathotype differential host system
  • Construction method of barley blumeria graminis pathotype differential host system

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Embodiment 1

[0017] Embodiment 1: (the construction method of the barley powdery mildew pathogenic type identification host system made up of 10 barley varieties)

[0018] (1) Collection of strains and classification of pathogenic bacteria toxicity types: In 2006, from the representative major barley producing areas in China with severe powdery mildew damage - Baoshan, Yunnan, Beifu, Chongqing, Jingzhou, Hubei, Zhumadian, Henan, Yancheng, Jiangsu, Zhejiang A total of 515 single-cell strains of barley powdery mildew were collected from Jiaxing and Putian, Fujian; 25 'Pallas' near-isogenic barley lines (Table 1) introduced from the Czech Republic were used as identification hosts to identify the pathogenic types of the above-mentioned single-cell strains. The identified strains showed differences in toxicity to P04B, P09, P10, P13, P17, P20, P23 and P24 in the near isogenic strain of 'Pallas', and were divided into 20 different pathogenic types; Among the pathogenic bacteria of different typ...

Embodiment 2

[0041] Embodiment 2: (comparison of discrimination between the 'Pallas' near-isogenic line of the present invention and the 'Pallas' introduced from abroad)

[0042] The identification results (Table 4) showed that 112 strains were used in the identification of hosts Shan'er, Dulihuang, Yundamai No. 2, E Damai No. 6, Supi No. 4, Beiqing No. 8, Pu Damai No. 7, and Zhe Nong Da No. 7. and Zhepi No. 3 showed differences in toxicity, and a total of 50 pathogenic types were identified. And these bacterial strains show toxicity difference to 8 strains in the near isogenic line of 'Pallas', distinguish 20 pathogenic types (table 2), increase 1 differential host, pathogenic type has increased by 1.5 times (theoretic maximum increase 1 times). From Table 2 and Table 4, strains from different regions, such as Shan'er, Dulihuang, Yundamai No. 2, E Damai No. 6, Supi No. 4, Beiqing No. 8, Pu Damai No. 7, Zhejiang Agricultural University No. 7 and Zhepi No. 3 were used as differential host...

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Abstract

The invention discloses a construction method of a barley blumeria graminis pathotype differential host system, belonging to the technical field of crop pathogenic bacteria pathotype identification. The method comprises the following steps of: (1) collecting strains and classifying pathogenic bacteria toxicity types; (2) collecting and identifying an alternative barley variety or strain; (3) measuring the disease resistance and the pathogenic bacteria pathogenicity of alternative differential hosts; (4) establishing the differential host system, and the like. In the invention, the barley blumeria graminis pathotype differential host system constructed by utilizing screened 10 varieties has stronger differentiation capacity, and classified pathotypes have obvious advantages. The method disclosed by the invention has stronger application values in main barley producing areas in China.

Description

technical field [0001] The invention relates to the technical field of pathogenic type identification of crop pathogenic bacteria, in particular to the establishment and application of a host system for identifying pathogenic type of barley powdery mildew. Background technique [0002] Barley is one of the main food crops in the world, and its sown area is second only to wheat, rice and corn, ranking fourth. my country's barley area and total production are second to rice, wheat, corn and millet, ranking fifth. Barley can be used as feed, food, beer and health food. Barley powdery mildew is one of the main diseases of barley. It mostly occurs in humid and semi-humid barley producing areas in the world. It occurs in most barley producing areas in China, mainly in winter barley areas, especially in the southwest and southeast coastal areas. The onset is severe. In recent years, due to the increase in the use of nitrogen fertilizers, the increase in planting density, the sin...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 杨建明朱靖环汪军妹贾巧君林峰华为尚毅
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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