Wheat molecular marker and application thereof in identifying wheat powdery mildew resistance

A technology of wheat powdery mildew and molecular markers, which can be applied to the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., and can solve the problems of frequent loss of resistance and variable pathogenic species

Active Publication Date: 2018-06-15
河南丰德康种业有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the variability of the pathogenic race, the use of a single main effect gene will result in the frequent loss of resistance with the large-scale promotion of bred varieties.

Method used

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  • Wheat molecular marker and application thereof in identifying wheat powdery mildew resistance
  • Wheat molecular marker and application thereof in identifying wheat powdery mildew resistance
  • Wheat molecular marker and application thereof in identifying wheat powdery mildew resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1. Obtaining the molecular marker IWB41105 of the powdery mildew resistance gene and its special primer set K-IWB41105 and Str-IWB41105.

[0066] Field phenotype identification and data analysis: F 1 Generation, continuous selfing to get F 8 Substituting groups of RILs, the F 8 A total of 244 families of RILs were planted in Zhengzhou and Beijing in 2015 and 2016 for field identification of adult plant resistance to powdery mildew. The international general SAS statistical software PROC CORR model was used to calculate the Pearson correlation coefficient of MDS, and the PROC ANOVA command was used for variance analysis. The results of correlation analysis and variance analysis showed that the correlation coefficients of the four environments were between 0.53-073, and the correlation was good. There are extremely significant differences among different genes in powdery mildew resistance, which clarifies the validity of phenotypic data.

[0067] Genetic map co...

Embodiment 2

[0079] Embodiment 2, establishment of genotype detection method

[0080] 1. Use the K-IWB41105 primer set of Example 1 to detect Zhou 8425B / Chinese Spring F 8 The genotypes of 244 families in the generation RIL population, and the F 1 generation as a comparison.

[0081] 1. Extract Zhou 8425B / Chinese Spring F 8 The genomic DNA of 244 families in the RIL population was generated to obtain a template solution with a DNA concentration of 100 ng / μl.

[0082] 2. Using the genomic DNA extracted in step 1 as a template, use the primer set K-IWB41105 for PCR amplification to obtain PCR amplification products, and use pure water to replace the template as a control.

[0083] 5μl system for PCR amplification of K-IWB41105 primer set: KASP mix 2.5μl, MgCl 2 0.04μl, FAM primer 134nM, HEX primer 134nM, common reverse primer 336nM, genomic DNA 100ng, with ddH 2 O supplemented to 5 μl. KASPmix (KBS-1016-002, KASP V4.0 2X Master mix 96 / 384, Std Rox) and MgCl 2 All are products of LGC....

Embodiment 3

[0117] Example 3. Using the K-IWB41105 primer set and the Str-IWB41105 primer set to detect the genotypes of 103 Zhou 8425B derivatives.

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PUM

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Abstract

The invention discloses a wheat molecular marker and application thereof in identifying wheat powdery mildew resistance. The wheat molecular marker disclosed by the invention comprises (M1) No.18 nucleotide, corresponding to a sequence 4 in a sequence table, in a wheat genome DNA (Deoxyribonucleic Acid); (M2) a DNA fragment containing the (M1). The invention also prepares a P primer group, a K-IWB41105 primer group and a Str-IWB41105 primer group for detecting the wheat molecular marker. The wheat molecular marker and the primer groups provided by the invention can be used for wheat powdery mildew adult-plant resistance molecular breeding and powdery mildew adult-plant resistance character identifying. The special primer provided by the invention plays an important role in wheat breeding for disease resistance.

Description

technical field [0001] The invention relates to the field of biotechnology, wheat molecular markers and their application in identifying wheat powdery mildew resistance. Background technique [0002] Wheat is the food crop with the largest planting area, the largest yield, the widest distribution and the highest trade value in the world and the most important grain source for human beings. At present, the annual planting area in the world exceeds 240 million hectares 2 , the output exceeded 600 million tons, and the trade volume was 173.5 million tons. my country is the country with the largest wheat production, and wheat is also the most important commodity grain and stored grain in my country. Ensuring high and stable wheat yields is not only a necessity for the basic needs of people's lives, but also a strategic choice for maintaining national food security. [0003] There are many factors affecting the high and stable yield of wheat, among which diseases are important ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/172
Inventor 郑天存贾奥琳夏先春何中虎
Owner 河南丰德康种业有限公司
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