Composite microbial inoculant for controlling plant continuous-cropping diseases and preparation method and application thereof

A compound bacterial agent and strain technology, applied in the directions of botanical equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., to achieve the effects of improving stress resistance, disease resistance, and fertilizer utilization.

Active Publication Date: 2015-04-22
中农绿康(北京)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] This application aims at the problems of pathological repeated cropping diseases caused by severe occurrence of pathogenic bacteria in the prior art, as well as physiological repeated cropping diseases caused

Method used

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  • Composite microbial inoculant for controlling plant continuous-cropping diseases and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] 1.1 Strain fermentation process

[0067] The production process of the compound preparation of Trichoderma harzianum, Bacillus subtilis and Glioma pink to prevent and control heavy cropping diseases is to make powder by liquid submerged fermentation method. The main steps of the process are the activation of the original strain → the expansion of the strain → the cultivation of the seed tank → fermentation Tank cultivation→filling material→stirring→chain machine crushing→powder sieving→product testing→packaging.

[0068] 1.1.1 Trichoderma harzianum

[0069] The strain patent preservation number of Trichoderma harzianum is CGMCC NO.7861. The production process is as follows:

[0070] 1.1.1.1 Strain activation

[0071] Trichoderma harzianum culture medium preparation: potato 20%, glucose 2%, agar 2%. Use NaOH or HCl to adjust the pH value to 7.0-7.5, then divide it into Erlenmeyer flasks or test tubes, place it in a sterilizer, and sterilize it under 0.11MPa pressure ...

Embodiment 2

[0131] Embodiment 2 (application composite bacterial agent is to strawberry growth promoting and disease prevention effect test)

[0132] Purpose of the test: In order to verify the effect of increasing yield and preventing disease of this product in a large-scale demonstration application on strawberries, a field plot test of this fertilizer was carried out on strawberries.

[0133] Test site: Xiaofengying Green Vegetable Garden Professional Cooperative, Kangzhuang Town, Yanqing County, Beijing

[0134] The composite microbial agent of Example 1 is selected: the ratio of Trichoderma harzianum, Glioma pink, Bacillus subtilis, phosphate solubilizing bacteria and potassium solubilizing bacteria is 1:1:3:2:2, and the bacterial content of the composite bacterial agent is, Bacteria content ≥ 500 million / g.

[0135] Tested variety: Strawberry variety "Hongyan"

[0136] Test method: The application method is hole application. There are 4 treatments in this test, namely: a1 conventi...

Embodiment 3

[0142] Embodiment 3 (application composite bacterial agent is to sugar beet root rot control effect and the test of increasing yield and increasing sugar)

[0143] Purpose of the test: In order to verify the effect of increasing yield and controlling root rot of this product in a large-scale demonstration application on sugar beet, a field test of this fertilizer was carried out on sugar beet.

[0144] Test site: sugar beet test field in Liangjia Village, Shangdu County, Ulanqab City, Inner Mongolia (heavy cropping field)

[0145] The composite microbial agent of Example 1 is selected: the ratio of Trichoderma harzianum, Glioma pink, Bacillus subtilis, phosphate solubilizing bacteria and potassium solubilizing bacteria is 1:1:3:2:2, and the bacterial content of the composite bacterial agent is, Bacteria content ≥ 500 million / g.

[0146] Test method: The application method is base application, and the treatments are compound bacterial agent and blank control. Each treatment is...

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Abstract

The invention provides a composite microbial inoculant for controlling plant continuous-cropping diseases, which comprises trichoderma harzianum, bacillus subtilis, and gliocladium roseum, and also comprises phosphate-solubilizing bacteria and potassium-solubilizing bacteria; the accession number of the trichoderma harzianum strain is CGMCC NO. 7861; the accession number of the bacillus subtilis strain is CGMCC NO. 7850; the accession number of the gliocladium roseum strain is CGMCC NO. 9008. The invention also provides a preparation method and an application of the composite microbial inoculant. Through the selection of high-efficient bacterial strains such as disease-preventing trichoderma, bacillus subtilis, gliocladium roseum, phosphate-solubilizing bacteria and potassium-solubilizing bacteria, the composite microbial inoculant of the invention promotes the growth of crops, improves the utilization rate of fertilizers, enhances the stress resistance such as disease resistance and the like, improves the quality of crops, and can control diseases such as root rot, verticillium wilt, damping off, botrytis, epidemic diseases, powdery mildew, sclerotinia rot, seedling-stage damping off, and the like.

Description

technical field [0001] The invention relates to a composite bacterial agent, in particular to a composite bacterial agent for preventing and treating plant cropping diseases, a preparation method and an application thereof. Background technique [0002] Heavy cropping disease refers to the disease caused by the long-term cultivation of the same crop on the same land. With the increasingly severe situation of desertification and desertification in the world, there is less and less arable land, and the contradiction between more people and less land in our country is more prominent. Multiple cropping index (cropping tillage) is the only way to solve the problem. However, the ensuing cropping diseases such as soil-borne diseases cause large-scale production reduction and extinction of crops, which is a devastating disease. Continuous cropping diseases include pathological continuous cropping diseases caused by serious occurrence of pathogenic bacteria, and physiological contin...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/20A01N63/04A01N63/02A01P3/00C12R1/885C12R1/125C12R1/645
CPCA01N63/10A01N63/30C12N1/145C12N1/205C12R2001/125C12R2001/645C12R2001/885A01N2300/00
Inventor 张丽霞
Owner 中农绿康(北京)生物技术有限公司
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