The invention relates to the microbial technology, in particular to a special micro organic complex fertilizer for red raspberries, as well as the preparation method thereof. The fertilizer matrix comprises the following materials (by weight): dried poultry manure of 60 to 70 percent and dry turfy soil of 30 to 40 percent, and microbe functional bacteria of 5 to 10 mL is sprayed in per 100 g of fertilizer matrix; the functional bacteria has the components by the volume ratio of azotobacter, phosphorus bacteria, silicate bacteria, bacillus licheniformis, bacillus subtilis and aeruginosa to be equal to 2-3:2.5-3.5:2-3:1-2:1-2:1.5-2.5. The prepared special micro organic complex fertilizer can be used for the cultivation of the red raspberries, after being used, the special micro organic complex fertilizer can improve the rhizosphere soil of the red raspberries, benefit the microbial ecosystem, restrain the pathogenic microorganism in the rhizosphere soil of the red raspberries, promote the plant growth and the fruit yield of the red raspberries, strengthen the antagonistic function of the red raspberries toward the stresses such as the pathogenic microorganism, drought, etc., and have well effects of resisting disease, increasing yield and increasing income, and all indexes reach the requirements of green food.