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Streptomyces microflavus

A technology of Streptomyces flavus and bacteria, applied in bacteria, fungicides, biocides, etc., can solve problems such as unsatisfactory disease prevention effect

Inactive Publication Date: 2010-10-20
GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a lot of work has been done on disease-resistant breeding, chemical control and agricultural measures at home and abroad, but the effect of disease prevention is not satisfactory
And due to the particularity of alfalfa as forage grass and factors such as economy, environment and food safety, there are strict restrictions on the use of chemical pesticides during its growth period.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, isolation and identification of bacterial strain

[0029] 1. Separation

[0030] Soil samples for testing: collected from the rhizosphere soil of healthy 2-3 year old alfalfa plants in Tongliao, Inner Mongolia.

[0031] Tested strains: provided by the Grassland Disease Control Group of the Grassland Research Institute of the Chinese Academy of Agricultural Sciences;

[0032] Medium: ①Seed medium: 5g peptone, 3g yeast extract, 2g glucose, 1g beef extract, PH7.0~7.2 per liter of water; ②Basic fermentation medium: 25g soybean powder, 40g starch, 0.8g ( NH 4 ) 2 SO 4 , 3 g CaCO 3 , 2g NaCl, pH7.0~7.2.

[0033] Preparation of soil sample dilution: Soil samples were collected from the rhizosphere soil of healthy 2-3 year old alfalfa plants in Tongliao, Inner Mongolia. When sampling, remove the surface plant residues, collect soil samples at a distance of 1-15 cm with a self-made soil sampler, and put them into a sterilized special aluminum dish. The soil...

Embodiment 2

[0132] Embodiment 2, the antimicrobial spectrum determination of bacterial strain

[0133] The antibacterial spectrum of strain NMG2-4-8 was determined by cup and saucer method. Put the sterilized Oxford cup into the center of the PDA plate mixed with the target bacteria (plate preparation method: add 1mL sterile water to each target bacteria culture test tube, scrape off the colonies, mycelia or spores with an inoculation loop to make a bacterial suspension mixed with PDA medium 10mL and poured into a 9cm Petri dish to make a plate), 200μL of sterile fermentation filtrate was added to the cup, and cultured at 25°C for 4-5d (fungus) and 28°C for 2d (bacteria) until The target bacteria overgrown the Petri dish. The diameter of the bacteriostatic (bacteria lysing) zone was measured by the cross method. Each target bacteria was repeated 3 times, and the results were averaged.

[0134] PDA agar medium: potato (peeled) 200g, sucrose (or glucose) 20g, distilled water 1000mL, pH n...

Embodiment 3

[0148] Embodiment 3, the greenhouse biological control plant disease experiment of bacterial strain NMG2-4-8

[0149] (Zhonglu No. 1) seeds were purchased from the National Forage Germplasm Medium-Term Bank, the product catalog number is 00068.

[0150] Alfalfa Peronospora Personspora aestivalis (Hou Tianjue. Investigation and main disease control of grassland diseases in northern my country [J]. China Grassland, 1993, 3: 56-60.) (Provided by Grassland Research Institute of Chinese Academy of Agricultural Sciences)

[0151] Alfalfa powdery mildew Erysiphe polygoni (Hou Tianjue. Occurrence status and control strategies of alfalfa diseases in my country [J]. Inner Mongolia Grass Industry, 1994, 3: 4-8.) (Provided by Grassland Research Institute of Chinese Academy of Agricultural Sciences)

[0152] Pseudopeziza medicaginis (Hou Tianjue. Investigation and control of grassland diseases in northern my country [J]. China Grassland, 1993, 3: 56-60.) (Provided by Grassland Research Ins...

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Abstract

The invention discloses a streptomyces microflavus. The streptomyces microflavus is the streptomyces microflavus NMG2-4-8, and the preservation number thereof is CGMCG No.3442. The streptomyces microflavus is separated and screened from alfalfa healthy tree rhizosphere soil for the first time in the invention, and experiments prove that the streptomyces microflavus has remarkable antagonistic action for 18 pathogenic fungi and 9 bacteria and broad antibacterial spectrum. The streptomyces microflavus has strong inhabiting action for crop pathogenic bacteria, such as alfalfa downy mildew and the like, shows a good disease prevention effect for the alfalfa downy mildew, powdery mildew, brown spots, stemphylium leaf spot disease, root rot disease and the like, and indicates that the bacterial strain has broad application prospect in the biological prevention and cure fields of alfalfa diseases.

Description

technical field [0001] The invention relates to a strain of Streptomyces luteus. Background technique [0002] Biological control of plant diseases emphasizes the virtuous cycle of the ecosystem and environmental protection, and can effectively avoid a series of problems caused by chemical pesticides. It has attracted more and more attention because of its social benefits, ecological benefits, and long-term benefits. As people pay more and more attention to the concepts of ecological balance and green health, biological control measures have received unprecedented attention in agricultural production. Since the beginning of the 21st century, governments and people of various countries have paid more attention to the development and application of biopesticides, and the share of biopesticides in the entire pesticide market is increasing year by year. In today's increasingly serious environmental pollution, biological control is the only way for agricultural development. Act...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00C12R1/465
Inventor 徐林波狄彩霞张星杰闫志坚高书晶赵海霞杨高鹏乌兰巴特尔李鹏戴雅婷孙娟娟李薇
Owner GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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