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Compound microbial bacteria and application thereof in prevention and treatment of vegetable fungal diseases

A microbial compound bacteria and compound bacteria technology, applied in the field of broad-spectrum microbial compound bacteria, can solve the problems of difficulty in the prevention or initial control of fungal diseases, difficulty in determining the disease, and narrow bacteriostatic spectrum, so as to promote sustainable development and ensure food safety. , the effect of improving product quality

Active Publication Date: 2014-06-18
河北绿茵生化科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is unrealistic to judge the possible disease of the plant when the plant is not diseased; moreover, even in the early stage of disease, because many fungal diseases have similar symptoms in the early stage, it is not easy to determine the specific disease infected by it, which gives the plant The prevention or initial control of fungal diseases brings great difficulties
However, the existing antagonistic preparations often have a narrow antibacterial spectrum, such as a compound bacterial agent disclosed in CN102827773, which is suitable for preventing and treating Botrytis cinerea

Method used

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  • Compound microbial bacteria and application thereof in prevention and treatment of vegetable fungal diseases
  • Compound microbial bacteria and application thereof in prevention and treatment of vegetable fungal diseases
  • Compound microbial bacteria and application thereof in prevention and treatment of vegetable fungal diseases

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preparation example Construction

[0035] The preparation method of the composite bacteria in each embodiment of the present invention is as follows: separately culture Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus brevis and Paenibacillus, and then mix them according to the ratio.

[0036] The cultivation methods of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus brevis and Paenibacillus in the present invention are well known to those skilled in the art.

Embodiment 1~4

[0038] Embodiment 1~4: The influence of different culture medium components on bacterial strain fermentation level

[0039] Among the present invention, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus brevis and Paenibacillus Y89 adopt the substratum components listed in Examples 1 to 4 to cultivate, and the substratum composition of Examples 1 to 4 is shown in Table 1, Each component was dissolved in water, and finally the volume was adjusted to 1L, and the pH was adjusted to 7.0±0.2. Each strain was cultured at 30°C with 200 rpm shaker. At the end of the culture period, the fermentation level of each bacterium reached 2-5×10 9 cfu / mL. If conventional LB or PB medium is used, the fermentation level of each bacteria is 0.1~1.8×10 9 cfu / mL. See Table 2 for the results of the cultivation.

[0040]

[0041]

Embodiment 5

[0042] Embodiment 5: compound bacterium and single bacterium application effect

[0043] The broad-spectrum microbial composite bacteria for preventing and treating fungal diseases of vegetables in this embodiment consists of the following four strains: Bacillus subtilis BSD-2, Bacillus brevis ch2, Bacillus amyloliquefaciens Y10 and Paenibacillus Y89. The bacterial counts of the above-mentioned various microorganisms in the composite bacteria (the cell numbers of different strains accounted for the percentage of the total cell number of the composite bacteria) were respectively: Bacillus subtilis BSD-2 accounted for 35%, Bacillus brevis ch2 accounted for 23% , Bacillus amyloliquefaciens Y10 accounted for 15%, Paenibacillus Y89 accounted for 27%, the total number of bacteria contained in the bacterial liquid was ≥2.0×10 9 cfu / mL.

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Abstract

The invention relates to broad-spectrum compound microbial bacteria for preventing and treating vegetable fungal diseases, and belongs to the technical field of agricultural biological prevention and treatment. The broad-spectrum compound microbial bacteria is compounded from four microorganisms, namely bacillus subtilis BSD-2 ((i) Bacillus subtilis), brevibacillus brevis ch2 ((i) Brevibacillus brevis), bacillus amyloliquefaciens Y10 ((i) bacillus amyloliquefaciens) and paenibacillus polymyxa Y89 ((i) Paenibacillus polymyxa) according to a certain ratio. By adopting the compound bacteria, the vegetable fungal diseases of a greenhouse or a large field, for example, botrytis, leaf mold, downy mildew, powdery mildew, blight and the like can be effectively prevented and treated, the field efficacy can be up to over 70%, the yield is increased by 5-20%, and the broad-spectrum compound microbial bacteria are significant in economic, social and ecological benefits, and have broad popularization and application prospects.

Description

technical field [0001] The invention relates to a microbial compound bacterium, in particular to a broad-spectrum microbial compound bacterium for preventing and treating vegetable fungal diseases. Background technique [0002] In recent years, greenhouse vegetables have developed rapidly due to their high yield and high efficiency, and the national planting area has reached more than 40 million mu. The unique temperature and humidity, continuous cropping and other environmental conditions in the shed make some fungal diseases such as botrytis cinerea, leaf mold, downy mildew, and fusarium wilt become serious diseases that endanger vegetable production. Most pathogenic fungi have the characteristics of wide parasitic range, fast reproduction speed, and easy genetic variation, which can easily cause a 20-40% reduction in vegetable production, and can reach more than 60% in severe cases. [0003] With the development of vegetable production, vegetable fungal damage occurs mor...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125C12R1/07
Inventor 张丽萍程辉彩尹淑丽张根伟李书生史延茂习彦花崔冠慧张飞燕董玉兰段普凡
Owner 河北绿茵生化科技有限公司
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