Bacillus velezensis HNt7 with broad-spectrum antibacterial activity as well as preparation method and application

A technology of bacillus, antibacterial activity, applied in the field of biological control of plant diseases

Active Publication Date: 2018-07-27
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no research on the use of this f

Method used

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  • Bacillus velezensis HNt7 with broad-spectrum antibacterial activity as well as preparation method and application
  • Bacillus velezensis HNt7 with broad-spectrum antibacterial activity as well as preparation method and application
  • Bacillus velezensis HNt7 with broad-spectrum antibacterial activity as well as preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0064] Pick a single colony of Bacillus Velez HNt7 in a sterile environment and inoculate it in a 100mL Erlenmeyer flask containing 25mL LB medium (10g peptone, 5g yeast extract, 10g NaCl, 1000ml distilled water, sterilized by high pressure steam at 121°C for 20min) placed in a constant temperature culture shaker NSKY-200B, adjust the temperature at 26-30°C, rotate at 150r / min, and shake for 24 hours. Each bottle is filled with 200mL fermentation broth, and the single colony liquid prepared in the previous step is added to each bottle at a ratio of 1:100, placed in a large-amplitude constant temperature refrigerated shaker, and shaken at 150r / min at 26-30°C for 48h.

[0065] A preparation method of Bacillus subtilis DYr3.3 with broad-spectrum antibacterial activity, the steps are:

[0066] A. Pick a single colony of Bacillus Velez HNt7 in a sterile environment and inoculate it in 100 mL of 25 mL of LB medium (10 g of peptone, 5 g of yeast extract, 10 g of NaCl, 1000 ml of dist...

Embodiment 2

[0073] The application of a broad-spectrum bacteriostatic activity of Bacillus velezensis in the preparation of medicine for treating or preventing pear fruit rot, the steps are:

[0074] A. Spraying period: In March or April every year, use a manual sprayer or a motorized sprayer to spray pesticides before 9:00 in the morning and after 4:00 in the afternoon on a sunny day or in cloudy weather (mainly to avoid high temperature time), evenly Spray the leaf surface. Spray once every 10 days and spray 3 times in a row. If there is heavy rain 24 hours after spraying, it should be applied again after the weather clears.

[0075] B. Dosage: Take the fermentation broth of Bacillus velezensis strain HNt7 and dilute it with water at a concentration of 100-500 times, 500ml per mu of land.

[0076] C. When spraying leaves, it cannot be mixed with copper preparations or bactericides.

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Abstract

The invention discloses bacillus velezensis HNt7 with broad-spectrum antibacterial activity as well as a preparation method and application. The antagonistic bacterium bacillus velezensis HNt7 has a number of CCTCC (China Center for Type Culture Collection) M 2018022. The preparation method comprises the following steps: A, selecting a bacillus velezensis HNt7 strain individual bacterial colony under a sterile condition and inoculating into a culture medium; adjusting the temperature and carrying out shake culture; B, filling a fermenting solution into each bottle and adding individual bacterial colony bacterium liquid according to the ratio; putting the bottle into a large-amplitude constant-temprature freezing shaking table and carrying out the shake culture; C, separating the antagonistic bacterium: immersing with alcohol with concentration and rinsing with sterile water; carrying out dark culture in a culture box and transferring a grown bacterial colony into a new culture medium and carrying out lineation separation and purification on the bacterial colony; D, after separating and purifying, obtaining the bacillus velezensis HNt7. The bacterium is used for preparing a medicinefor treating or preventing pear fruit rot disease. The strain has a remarkable disease-prevention effect and the prevention effect reaches 70.76 percent or more. After the bacillus velezensis HNt7 isapplied to pear trees, advanced defoliation of the pear trees is remarkably inhibited and the tree vigor is easy to enhance.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and more specifically relates to a broad-spectrum antibacterial activity of Bacillus Velez HNt7, and also relates to a preparation method of a broad-spectrum antibacterial activity of Bacillus Velez HNt7. The invention relates to the use of a broad-spectrum antibacterial activity of Bacillus velei HNt7. Background technique [0002] Pear tree rust is caused by Gynmosporangium haraeanum Syd.=Gynmosporangium asiaticum Miyabe ex Yamada, which is common in Chinese pear orchards and can cause leaves and fruits to fall off, seriously affecting yield. At present, chemical agents are mainly used for prevention and control in China, such as mancozeb, diniconazole, triadimefon, etc. In addition to reports, biological control is carried out by hyperparasites of pear gum rust (Tuberculina vinosa Sacc.) In addition to control, there is no commercial biocontrol agent. Long-term a...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCA01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 徐文兴赵梓茜郭大勇尤华锋王利平洪霓王国平
Owner HUAZHONG AGRI UNIV
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