A kind of Bacillus hnt7 with broad-spectrum antibacterial activity and its preparation method and application

A kind of antibacterial activity, Bacillus technology, applied in the field of plant disease biological control

Active Publication Date: 2019-12-24
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the use of this fungus for the control of pear rust at home and abroad.

Method used

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  • A kind of Bacillus hnt7 with broad-spectrum antibacterial activity and its preparation method and application
  • A kind of Bacillus hnt7 with broad-spectrum antibacterial activity and its preparation method and application
  • A kind of Bacillus hnt7 with broad-spectrum antibacterial activity and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Pick a single colony of Bacillus Velez HNt7 strain in a sterile environment and inoculate it in a 100 mL Erlenmeyer flask containing 25mL LB medium (10g peptone, 5g yeast extract, 10g NaCl, 1000ml distilled water, autoclaved at 121°C for 20min) Place it in a constant temperature culture shaker NSKY-200B, adjust the temperature to 26-30℃, rotate the speed at 150r / min, and shake culture for 24h. Each bottle contains 200mL fermentation broth, and each bottle is added with the single colony broth prepared in the previous step at a ratio of 1:100, placed in a large amplitude constant temperature freezing shaker, and cultured with shaking at 150r / min at 26-30°C for 48h.

[0065] A preparation method of Bacillus subtilis DYr3.3 with broad-spectrum antibacterial activity, the steps are:

[0066] A. Pick a single colony of Bacillus Velez HNt7 strain in a sterile environment, and inoculate it in 100 mL of 25 mL LB medium (10 g peptone, 5 g yeast extract, 10 g NaCl, 1000 ml distilled w...

Embodiment 2

[0073] The application of Bacillus velezensis with broad-spectrum antibacterial activity in the preparation of a medicine for treating or preventing pear fruit rot disease, the steps are:

[0074] A. Application period: In March or April of each year, use a manual sprayer or a motorized sprayer to spray and apply the spray evenly before 9 o'clock in the morning and after 4 o'clock in the afternoon on a sunny day or in cloudy weather (mainly to avoid high temperatures) Spray the surface of the blade. Spray once every 10 days and spray 3 times in a row. If there is heavy rain 24 hours after spraying, it should be applied again after clearing.

[0075] B. Dosage: Take the fermentation broth of Bacillus velezensis strain HNt7 and dilute it with water at 100-500 times the concentration, 500ml per mu.

[0076] C. When spraying leaves, it cannot be mixed with copper preparations or bactericides.

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Abstract

The invention discloses a Bacillus Velez HNt7 with broad-spectrum antibacterial activity, a preparation method and application thereof, and antagonizes the Bacillus Velez HNt7, and the serial number is CCTCC M 2018022. The steps are: A. Pick a single colony of Bacillus Velez HNt7 in a sterile environment, inoculate it in the culture medium, adjust the temperature, and shake it for culture; B. Add a single colony liquid to each bottle of fermentation liquid according to the proportion, and place it in a large Vibrating culture in a constant-temperature shaker with constant temperature; C. Isolation of antagonistic bacteria: Soak in concentrated alcohol, rinse with sterile water, culture in an incubator in dark, and transfer the grown bacterial colonies to a new medium Separation and purification of a single colony by streaking; D. After separation and purification, Bacillus Velez HNt7 was obtained. Application of the bacterium in preparing medicine for treating or preventing pear fruit rot disease. The strain has significant disease prevention effect, and the control effect reaches more than 70.76%. The pear trees sprayed with Bacillus Velez HNt7 significantly inhibited the premature defoliation of the pear trees, which was beneficial to enhance the tree vigor.

Description

Technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and more specifically relates to a method for preparing Velez Bacillus HNt7 with broad-spectrum antibacterial activity, and also relates to a preparation method of Velez Bacillus HNt7 with broad-spectrum antibacterial activity. It relates to the use of Bacillus velez HNt7 with broad-spectrum antibacterial activity. Background technique [0002] Pear rust is caused by Gynmosporangium haraeanum Syd (= Gynmosporangium asiaticum Miyabe ex Yamada), which is more common in Chinese pear orchards, and can cause leaf and fruit shedding, seriously affecting yield. At present, China mainly uses chemical agents for prevention and control, such as mancozeb, diniconazole, triadimefon, etc. In addition to reports that the biological prevention and control uses tuberculina vinosa Sacc. Outside of control, there is no commercial biocontrol agent. Long-term application of chemical pestici...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCA01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 徐文兴赵梓茜郭大勇尤华锋王利平洪霓王国平
Owner HUAZHONG AGRI UNIV
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