Bacillus subtilis and microbial agent containing same

A technology of Bacillus subtilis and biological inoculants, applied in the directions of fungicides, bacteria, biocides, etc., to achieve the effect of facilitating storage and transportation, long shelf life, and avoiding residues and contamination

Active Publication Date: 2014-05-14
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are relatively few studies on the biological control of peanut fruit rot. Therefore, it is urgent to screen out a microbial strain and related microbial agents that can efficiently antagonize the pathogenic bacteria of peanut fruit rot, so as to reduce peanut fruit rot through biological control. Harm caused by disease to peanut production

Method used

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  • Bacillus subtilis and microbial agent containing same
  • Bacillus subtilis and microbial agent containing same
  • Bacillus subtilis and microbial agent containing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Isolation and Screening of Bacteria with Antagonism to Peanut Fruit Rot

[0024] The rhizosphere soil of healthy peanut plants in the field with severe peanut fruit rot was collected as the test material. Bacteria were isolated from the soil samples in the laboratory, and the bacteria were isolated and purified on LB medium for many times to obtain pure culture bacteria. , using fusarium of peanut fruit rot as the indicator bacteria, the confrontation test method was used to screen the strains with high antagonistic performance.

[0025] Specific steps: The above soil samples come from peanut fields infected with peanut fruit rot in Hebei Province, Ningxia, Shandong, Shanxi and other places. The soil is diluted in a gradient and spread in PB medium, and cultured in a constant temperature incubator at 28°C . The above-mentioned medium was prepared according to the records in the "Microbial Culture Medium Manual". The formula of the medium is: 5g of beef extrac...

Embodiment 2

[0027] Example 2 Determination of the genetic stability of antagonistic strains

[0028] The strain with the strongest antagonistic effect was subcultured on PB medium by streaking method. After 15 generations of culture, the antagonistic effect of the strain on Fusarium fusarium of peanut fruit rot was determined by plate confrontation method, and the original strain was used as the control , the antagonistic strain has the same antagonistic effect as the original strain after 15 generations of culture, indicating that the antagonistic strain has good genetic stability, and the antagonistic strain with stable antibacterial effect is cultured in PB medium, and the bacterial liquid is drawn for -80 ℃ glycerol storage.

Embodiment 3

[0029] Example 3 Identification of antagonistic strains

[0030] According to the experimental content and experimental methods recorded in the "Common Bacteria System Identification Manual", the screening of the preservation number: CGMCC No.8340 was identified, and the observation of the colony morphology was carried out on the PB medium. After the strain was streaked and inoculated , incubate at 28°C for 24 hours to observe the colony morphology. The colonies of the strain with the preservation number CGMCC No.8340 are nearly round, with gnawing edges, umbilicus-like protrusions, wrinkled surfaces, milky white color, dull and opaque, and are not tightly combined with the medium. Pick and culture the bacterial lawn for 24 hours and observe it under the 100 times oil lens of an optical microscope after Gram staining. It can be seen under a microscope that the strain with the preservation number CGMCC No.8340 is a bacillus, a Gram-positive bacterium, with mesophytic or near-...

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Abstract

The invention provides bacillus subtilis with the preservation number of CGMCC No.8340, a microbial agent containing the bacillus subtilis and an application of the bacillus subtilis or the microbial agent in the prevention and control of peanut fruit rot. The bacillus subtilis with the preservation number of CGMCC No.8344 and the microbial agent containing the bacillus subtilis have good antagonistic effects on the peanut fruit rot bacteria; a screened bacillus subtilis strain can be used for preparing the corresponding microbial agent, and after the prepared microbial agent is applied to a peanut planting environment, the occurrence of the peanut fruit rot can be prevented and controlled under the action of the bacillus subtilis, so that the application of chemical pesticides in a peanut production process is reduced.

Description

technical field [0001] The invention relates to a bacillus subtilis BS-KA2 and a biological agent for preventing and treating peanut fruit rot caused by fusarium infection, belonging to the field of biotechnology. Background technique [0002] Fusarium is a worldwide distribution of fungi, it can not only survive winter and summer in the soil, but also infect a variety of plants (food crops, economic crops, medicinal plants and ornamental plants), causing plant root rot, stem There are more than 100 species of host plants, infecting the vascular system of host plants, destroying the vascular bundles of plant transport tissues, and producing toxin hazards in the process of growth, development and metabolism Crops, causing crop wilting and death, affecting yield and quality, is one of the most difficult important diseases to control in production. [0003] Fusarium solani is a kind of Fusarium fungus, which reproduces in large quantities in the soil of heavy cropping peanuts,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125
Inventor 贾振华刘昆昂黄亚丽宋聪马宏赵芊黄媛媛宋水山李书生
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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