Butterfly orchid tissue culture seedling breeding method

Abstract

The invention discloses a butterfly orchid tissue culture seedling breeding method which includes the steps: (1) inoculating butterfly orchid pedicel sections with dormant buds onto a cluster bud induction medium; (2) cutting the cluster buds, and inoculating the cut cluster buds into a proliferation medium, culturing the cluster buds by an LED (light-emitting diode) red light source, and transferring the cluster buds to culture the cluster buds by a natural light source; (3) dividing the cluster buds into single buds, inoculating the single buds into a root tip induction medium, and culturingthe single buds by an LED composite light source; (4) transmitting the root tip induction medium into a greenhouse with a sunshade net to exercise seedlings by natural light; (5) wrapping a butterflyorchid plantlet root systems with sphagna, and placing butterfly orchid plantlets into the greenhouse to culture the plantlets; (6) beginning to apply fertilizers when new roots grow out; (7) changing the sphagna into nutrition pots with the sizes of 3.5 inches when the sphagna are completely wound by the root systems; (8) applying the fertilizer once every ten days after the pots are replaced for ten days. The method for breeding the butterfly orchid tissue culture seedlings can effectively solve the problems that the reproduction rate of the cluster buds is low, rooting rate is low, butterfly orchid plants are yellowed as roots are easily rotted after the plants are transplanted and the like, and the production cost of the butterfly orchid tissue culture seedlings can be reduced.

Description

technical field [0001] The invention belongs to the field of flower nursery stock breeding, in particular to a method for breeding moth orchid tissue culture seedlings. Background technique [0002] Phalaenopsis is a single-stem aerial orchid, which rarely develops side branches and is difficult to carry out conventional asexual reproduction; in addition, Phalaenopsis cannot complete pollination under natural conditions, and it is difficult to obtain seeds. Even if artificial pollination is used to obtain seeds, the seeds do not contain Endosperm is also difficult to germinate under natural conditions, and the germination rate is extremely low, so it is difficult for Phalaenopsis to use seeds for sexual reproduction. At present, the rapid propagation of Phalaenopsis is mainly carried out by tissue culture method. The tissue culture of Phalaenopsis can obtain a large number of high-quality and neat young Phalaenopsis plants in a short period of time. It has high proliferation...

AI Technical Summary

Problems solved by technology

[0003] But there are following problems in existing Phalaenopsis tissue culture seedling breeding: the first, when Phalaenopsis adopts approaches such as callus to induce adventitious buds to propagate, progeny variation will occur in the offspring, resulting in not being able to keep the excellent traits of the parent

Second, the traditional light source for Phalaenopsis tissue culture is a white fluorescent lamp, which generates a lot of heat. A large number of fluorescent lamps are used as the light source, which increases the cost of indoor temperature control; and the light utilization rate is low, which greatly wastes resources.

Third, when white fluorescent lamps are used as light sources for Phalaenopsis tissue culture, the proliferation rate of clustered buds and the number of roots are low

Fourth, Phalaenopsis tissue culture seedlings are grown in an environment that is airtight and sterile, with sufficient nutrient supply, suitable light, and constant temperature and humidity. Poor adaptability, resulting in low transplant survival rate

At present, sphagnum moss imported from Chile is used as the main cultivation substrate for Phalaenopsis tissue culture seedlings. Sphagnum moss imported from Chile can better solve the problem of air permeability of the cultivation substrate. However, as the cultivation time increases, the cultivation substrate composed of sphagnum moss imported from Chile alone There will be acidification and rot, which will easily cause root rot of Phalaenopsis tissue culture seedlings; at the same time, due to the rising price of Chilean imported sphagnum moss, the production cost of a single Phalaenopsis seedling is high