High yield cell wall degrading enzyme and Trichoderme viride of antibacterial peptide and biocontrol reagent thereof

A technology of trichoderma viridans and reagents, applied in biocides, bactericides, biocides, etc., to achieve broad development prospects, improve food safety, and improve the ecological environment

Inactive Publication Date: 2010-06-09
HANGZHOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the application of biological control can be regarde

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 975 parts by weight of Trichoderma viride TV501 fermentation liquid and 25 parts by weight of Tween-2025 were uniformly mixed to obtain a biocontrol agent, and the fermentation liquid was obtained by shaken flask vibration culture, wherein the formula of the fermentation medium was:

[0035] NaNO 3 1.8g / L, K 2 HPO 4 0.5g / L, KCl 0.5g / L, MgSO 4 0.4g / L, FeSO 4 0.01g / L, sucrose 30g / L, bran extract 380ml / L. The bran extract was boiled at 90° C. for 1 hour by placing 50 g of bran in 1.5 L of water, and distilled to 450 ml with water after filtering. After fermentation, the fermentation liquid was measured by filter paper enzyme activity and antimicrobial peptide activity immunoassay, and obtained the cell wall degrading enzyme activity 550.7U / ml (representing the enzyme activity unit per milliliter, taking the enzyme amount that catalyzes cellulose hydrolysis to generate 1 μ mol glucose per minute as 1 Enzyme activity unit (i.e. 1U), antimicrobial peptide activity 500...

Embodiment 2

[0041] Mix 487.5 parts by weight of Trichoderma viride TV501 fermented liquid, 487.5 parts by weight of Tween-2025 and 487.5 parts by weight of water, and stir evenly to obtain a biocontrol reagent.

[0042] Ca(NO 3 ) 2 4H 2 O 0.28g / L, KNO 3 0.08g / L, KCl 0.06g / L, MgSO 4 7H2O0.36g / L, NaH 2 PO 4 .2H 2 O 0.02g / L, Soytone 1g / L, Ammonium Tartrate 5g / L, FeCl 3 ·6H 2 O2mg / L, MnSO 4 .H 2 O 5mg / L, ZnSO 4 ·7H 2 O 2.5mg / L, H 3 BO 3 1.4mg / L, KI0.7mg / L, yeast extract 0.25g / L, bran extract 450ml / L, the bran extract was boiled by putting 1g bran in 1.5L water at 90°C for 1 hour, and filtered Make up to 450ml with water. After fermentation, the fermented liquid was measured by filter paper enzyme activity and antimicrobial peptide activity immunoassay, and obtained cell wall degrading enzyme activity 580.5U / ml (representing the unit of enzyme activity per milliliter, taking the enzyme amount that catalyzes cellulose hydrolysis to generate 1 μ mol glucose per minute as 1 Enzym...

Embodiment 3

[0050] 975 parts by weight of Trichoderma viride TV501 fermented liquid and 975 parts by weight of Triton-10025 parts by weight were uniformly mixed to obtain a biocontrol agent, and the fermented liquid was obtained by shaking and fermenting in a shaker flask, wherein the medium formula of Trichoderma viride TV501 fermented liquid was:

[0051] NaNO 3 1.5g / L, K 2 HPO 4 1.0g / L, KCl 0.4g / L, MgSO 4 0.5g / L, FeSO 4 0.012g / L, sucrose 25g / L, bran extract 420ml / L. The bran extract was boiled at 90° C. for 1 hour by placing 50 g of bran in 1.5 L of water, and distilled to 450 ml with water after filtering. After fermentation, the fermentation liquid was measured by total enzyme activity and immunodetection of antimicrobial peptide activity, and obtained cell wall degrading enzyme activity 551.2U / ml (representing the enzyme activity unit per milliliter, taking the enzyme amount that catalyzes cellulose hydrolysis to generate 1 μ mol glucose per minute as 1 Enzyme activity unit ...

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PUM

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Abstract

The invention discloses a high yield cell wall degrading enzyme and Trichoderme viride of antibacterial peptide, which is named Trichoderma viride TV 501, and has a preservation number reading: CCTCC No:M209321.The Trichoderme viride can produce cell wall degrading enzyme and antibacterial peptide in large quantities, the biocontrol reagent made from the Trichoderme viride have relatively good field prevention effect on vegetable and fruit diseases such as strawberry powdery mildew, grey mould fruit rot of strawberry, powdery mildew of cucumber and the like, obviously improves yield and quality of the vegetables and fruits, reduces use amount of chemical pesticide, improves the ecological environment, enhances food security, demonstrates vast potential for future development and is conducive to enhancing agricultural efficiency and increasing the income of the farmers.

Description

technical field [0001] The invention relates to a fungus, in particular to a Trichoderma viride strain with high production of cell wall degrading enzymes and antibacterial peptides and a biocontrol agent thereof. Background technique [0002] In the 21st century, with the development of the economy and the continuous improvement of people's living standards, people's awareness of health and environmental protection has been greatly enhanced. It has become an inevitable trend in the development of pesticides to reduce the use of chemical pesticides and use green biological pesticides to control diseases, insect pests and weeds. my country is a large agricultural country, and the area affected by crop diseases, insect pests and weeds is about 200 million hectares (times) every year, and about 250,000 tons of pesticides (active ingredients) need to be produced and used every year. Among the pesticides used, chemical pesticides account for a considerable proportion, while biol...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 马华升阮松林杨平平忻雅童建新王淑珍余红来文国郑桂珍
Owner HANGZHOU ACAD OF AGRI SCI
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