Nondestructive monitoring method and system for judging healing state of muskmelon grafted seedling by using wearable sensor

Abstract

The invention belongs to the technical field of plant monitoring, and particularly relates to a nondestructive monitoring method and system for judging the healing state of a muskmelon grafted seedling by using a wearable sensor, and the method comprises the steps: preparing a pumpkin rootstock and a muskmelon scion for grafting; grafting when the first true leaf of the pumpkin rootstock is completely flattened and the first true leaf of the scion is half-expanded; a sensor is attached to the cotyledon of the scion to continuously detect the growth state of the grafted seedling; impedance signals of the sensor are measured through an instrument, and an impedance change curve 10 days after grafting is drawn to evaluate the grafting healing state. According to the method, the physiological activity state of the grafted seedling can be directly monitored, so that whether the healing state is good or not can be judged in advance.

Description

Technical Field

[0001] The invention belongs to the technical field of plant monitoring, and in particular relates to a non-destructive monitoring method and system for judging the healing state of a melon grafted seedling by using a wearable sensor. Background Art

[0002] Grafting: It is a method of reproduction in which a bud or branch of one plant is grafted onto another plant so that the two parts grow into a complete plant. The grafted bud or branch is called a scion, and the grafted plant is called a stock. The key to a successful graft is to tightly combine the cambium of the scion and the stock.

[0003] Graft healing: Graft healing is an important stage in the survival of grafted plants and is the process by which the rootstock and scion combine into a complete organism (Nawaz et al 2017).

[0004] Graft healing detection: Judging the healing status of the graft during the grafting process is conducive to early judgment of the growth of the grafted seedlings and improving the efficiency of intensive management of grafted seedlings. The traditional judgment of the healing status of grafted seedlings is mainly based on experienced agronomists observing the growth of the true leaves of the scion, which consumes a lot of manpower and material resources and is costly (Gu Song et al. 2006). The methods widely used in research, such as fluorescent staining (XU JN et al2022), paraffin section (TANG Dan 2016), and anatomical analysis (Wu Ye et al. 2020), are complex, costly, time-consuming and labor-intensive. Most importantly, the above methods are all artificial destructive methods. The grafted seedlings cannot continue to survive and grow after the healing test, so it is difficult to achieve industrial application.

[0005] Non-destructive testing of grafted seedlings: With the rapid development of sensor detection, image recognition, computer image processing and other technologies, in order to improve the production efficiency and economic benefits of grafted seedlings, many studies are currently devoted to applying machine vision technology to non-destructive testing of grafted seedlings (Cui Yongjie et al. 2014; Teng Guanghui and Li Changying 2002). Although the impact on plant growth can be minimized by detecting the healing state of grafted seedlings through non-contact methods such as image vision technology, it is easily affected by external environmental factors such as light and the state of the grafted seedlings during machine detection, resulting in inaccurate results.

[0006] Plant flexible wearable sensors: Sensors made of flexible materials have excellent flexibility, ductility and biocompatibility. They can be directly attached to the surface of plants for data collection without affecting the normal growth of the plants. Currently, a variety of flexible and multifunctional plant flexible sensors have been developed. Flexible sensors can be used to achieve in-situ non-destructive monitoring of plant status.

[0007] Laser-induced graphene: The basic principle of laser-induced graphene is to use the high energy density of laser to locally carbonize carbon-based materials and transform them into graphene structures. Graphene is a single layer of hexagonally arranged carbon atoms with excellent flexibility and high conductivity.

[0008] Interdigitated electrode: It is a common electrode structure, widely used in electrochemistry, sensors, biosensors and micro-nanotechnology. The design of interdigitated electrode usually consists of two sets of staggered electrode fingers, similar to two rows of fingers staggered together, hence the name "interdigitated".

[0009] At present, there are few reports on non-destructive detection technology for the healing state of melon grafted seedlings. Generally speaking, destructive monitoring methods are used, such as observation of healing tissue sections, fluorescent staining, etc. Today, plant sensor technology is developing rapidly, and many plant sensors have been developed, but there are no reports on the application of sensor technology to detect the healing state of melon grafted seedlings. Summary of the invention

[0010] In view of the problems existing in the prior art, the present invention provides a non-destructive monitoring method and system for judging the healing status of melon grafted seedlings by using a wearable sensor.

[0011] The present invention is implemented as follows: a non-destructive monitoring method for judging the healing state of melon grafted seedlings using a wearable sensor, the method comprising:

[0012] S1, pumpkin rootstock and melon scion prepared for grafting;

[0013] S2, grafting is done when the first true leaf of pumpkin rootstock is fully unfolded and the first true leaf of scion is half unfolded;

[0014] S3, attaching the sensor to the cotyledons of the scion to continuously detect the growth status of the grafted seedling;

[0015] S4, the impedance signal of the sensor is measured by the instrument, and the impedance change curve 10 days after grafting is drawn to evaluate the grafting healing status.

[0016] Further, in S1, preparing pumpkin stock for grafting includes: disinfecting pumpkin stock seeds with 1‰ potassium permanganate solution for 10 minutes, washing with tap water for 3 times, and then soaking the seeds in 55-60°C warm water for 6-8 hours; then placing the seeds in a culture dish covered with moist gauze and covering with plastic wrap to keep moisture, placing in a 30°C incubator for germination for 24 hours, sowing in a 50-hole plug tray when the seeds turn white, and grafting when the first true leaf is fully unfolded.

[0017] Furthermore, in S1, preparing the melon scion for grafting includes: disinfecting the melon scion seeds with 1‰ potassium permanganate solution for 10 minutes, washing with tap water for 3 times, and then soaking the seeds in 55-60°C warm water for 6-8 hours; then placing the seeds in a culture dish covered with moist gauze, covering with plastic wrap to keep moisture, placing in a 30°C incubator for germination for 24 hours, sowing in a 100-hole tray when the seeds are white, and grafting when the first true leaf is half-expanded.

[0018] Further, the S2 includes:

[0019] Stock processing includes: using a blade to cut off the true leaves, growth points and one cotyledon at an angle of 45 degrees from the base of the cotyledon, leaving only one cotyledon; keeping the cut surface at 0.5 cm long, and completing it in one cut as much as possible to ensure a smooth cut surface;

[0020] The scion processing includes: using a blade to make an oblique cut at 45 degrees upwards at about 1.0 cm from the base of the cotyledons, and the cut surface is kept at 0.5-1.0 cm and is smooth.

[0021] Furthermore, the substrate was watered thoroughly one day before grafting, and the blades, grafting clips and grafting healing environment used for grafting were sterilized with 75% medical alcohol in advance and used after natural drying.

[0022] Furthermore, in S3, the requirement for the grafted seedlings is: continuous monitoring of a single plant is required, and approximately 10 grafted seedlings need to be monitored to complete the healing state curve.

[0023] Further, in S3, firstly, during the grafting healing period, the grafted seedlings will be in a small closed environment with high humidity. When collecting data, we need to transfer the grafted seedlings to an environment with environmental parameters of humidity 55%-60% and temperature 23°C-26°C, and collect data after 3min-4min.

[0024] The data collection process is as follows: first, the electrodes are attached to the surface of the scion leaves, and then the timing is started. After 1 minute, the data is almost stable, and the data is recorded at this time; first, several grafted seedlings are prepared. After grafting, the day of grafting is day 0, and the leaves of the scion are monitored and data is collected from the first day.

[0025] Furthermore, in S4, the graft healing state is evaluated by the inflection point of the descending curve: the earlier the inflection point, the better the graft healing state.

[0026] The present invention provides a nondestructive monitoring system for judging the healing state of a melon grafted seedling using a wearable sensor, which implements the nondestructive monitoring method for judging the healing state of a melon grafted seedling using a wearable sensor. The system is composed of four layers of materials stacked in sequence from outside to inside:

[0027] The four layers of materials include: 1. Flexible stretchable adhesive layer; 2. Carbon precursor material layer; 3. Conductive material layer; 4. Porous material functional layer. The flexible stretchable adhesive layer is a polydimethylsiloxane layer, the carbon precursor material layer is a polyimide layer, the conductive material layer is a graphene layer, and the porous material functional layer is a sheet-like conductive nanomaterial layer. In the present invention, polydimethylsiloxane is used as the flexible stretchable adhesive layer, polyimide is spin-coated on the flexible stretchable adhesive layer and heated and cured to form a carbon precursor layer, laser-induced graphene technology is used to generate interdigitated electrodes on the carbon precursor layer to form a conductive material layer, and finally sheet-like conductive nanomaterials are deposited on the conductive material layer to form a porous material functional layer.

[0028] The grafting preparation module prepares pumpkin rootstock and melon scion for grafting; grafting is performed when the first true leaf of the pumpkin rootstock is completely flattened and the first true leaf of the scion is half-opened;

[0029] The state detection module is connected to the grafting preparation module, and the sensor is attached to the cotyledons of the scion to continuously detect the growth state of the grafted seedling;

[0030] The evaluation module is connected to the status detection module, measures the impedance signal of the sensor through the instrument, and draws the impedance change curve 10 days after grafting to evaluate the grafting healing status.

[0031] In combination with the above technical solutions and the technical problems solved, the advantages and positive effects of the technical solutions to be protected by the present invention are as follows:

[0032] First, the present invention can directly monitor the physiological activity state of the grafted seedlings, so as to determine in advance whether the healing state is good. According to the transpiration rate of the scion leaves, we can judge the process of healing and connectivity of the grafted seedlings. Monitoring leaf transpiration utilizes an interdigitated electrode prepared based on polyimide, which can be attached to the surface of the scion leaves to collect impedance data and draw the impedance signal change curve of the grafted seedlings with good healing state. In this way, the healing state of the grafted seedlings can be judged non-destructively.

[0033] Second, the expected benefits and commercial value of the technical solution of the present invention after transformation are:

[0034] In the factory production of grafted seedlings, the judgment of the healing status of grafted seedlings is a key link in the production chain of grafted seedlings, which directly affects the quality of factory seedlings. At present, the judgment of the healing status of grafted seedlings mainly relies on experience judgment, which has problems such as strong subjectivity and susceptibility to human habit preferences. It may misjudge the healing status of grafted seedlings and traditional experience requires 10 days after grafting to make an effective judgment, which will also affect the factory production efficiency. Therefore, the traditional judgment method is difficult to meet the requirements of modern grafted seedling production lines. Therefore, it is of positive significance to develop a method for detecting the healing status of grafted seedlings to replace manual labor, which can accurately and stably judge the healing status of grafted seedlings and judge the healing time in advance, thereby improving the turnover rate of automated healing equipment and reducing enterprise production costs.

[0035] The technical solution of the present invention fills the technical gap in the industry at home and abroad:

[0036] In terms of non-destructive testing of the healing state of grafted seedlings, there are also related studies at home and abroad, which use machine vision technology and hyperspectral fluorescence imaging methods for non-destructive testing of the healing state of grafted seedlings. Although the impact of the healing state of grafted seedlings on plant growth can be minimized by non-contact methods such as image vision technology, it is easily affected by external environmental factors such as light and the state of the grafted seedlings during machine testing, resulting in inaccurate results.

[0037] Third, the non-destructive monitoring method of the present invention effectively solves the problem that it is difficult to accurately and real-time judge the healing state of melon grafted seedlings in the prior art by introducing impedance sensing technology and real-time data collection, and has made significant technical progress in parameter optimization and application, which is mainly reflected in the following aspects:

[0038] 1. Non-destructive monitoring improves the accuracy of healing detection of grafted seedlings

[0039] The existing assessment of the healing status of melon grafted seedlings usually relies on naked eye observation or mechanical means, which makes it difficult to achieve continuous and real-time monitoring of the healing status. The present invention uses impedance sensing technology combined with a continuous impedance change curve to detect the physiological changes of the grafted seedlings in real time after grafting and provide accurate feedback on the healing status. By detecting the inflection point of the impedance curve, the present invention achieves accurate assessment of the healing status.

[0040] 2. Improved the scientific management level during the grafting process

[0041] The present invention effectively improves the standardization and scientificity of grafting operation by accurately controlling the grafting time, grafting angle, humidity, temperature and other conditions, as well as disinfecting the grafting tools, fixtures and environment. In every link of the entire grafting and healing process, accurate management based on data can greatly improve the grafting survival rate, reduce unnecessary losses, and improve the efficiency of grafting operation.

[0042] 3. Automated data collection enables continuous monitoring of healing status

[0043] By attaching sensors to the cotyledons of the scion and continuously collecting impedance data under set environmental conditions, the present invention can automatically generate an impedance change curve of the healing state of the melon grafted seedlings, realizing non-destructive monitoring of the healing state of the grafted seedlings. This technology breaks through the limitation of the existing technology that relies on manual monitoring, and can perform large-scale, automated detection, significantly improving the automation level of grafted seedling management.

[0044] 4. Impedance curve analysis technology solves the problem of quantifying the graft healing process

[0045] In the present invention, by analyzing the inflection point of the impedance curve, the healing process of the grafted seedling can be quantified. The earlier the inflection point appears, the better the grafted healing. This parameterized evaluation method solves the problem that traditional naked eye observation is not accurate enough, so that the healing state of the grafted seedling can be intuitively displayed and quantitatively evaluated through specific curve data, thereby improving the scientific nature of the healing state judgment.

[0046] In summary, the present invention has made significant progress in the non-destructive monitoring technology of melon grafted seedlings, provided an efficient and accurate detection method, improved the scientificity and automation level of grafted seedling management, and provided reliable technical support for the production and management of melon grafted seedlings. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] Figure 1 It is a flow chart of a nondestructive monitoring method for determining the healing state of a melon grafted seedling using a wearable sensor provided by an embodiment of the present invention;

[0048] Figure 2 is a structural diagram of a nondestructive monitoring system for determining the healing state of a grafted melon seedling using a wearable sensor provided by an embodiment of the present invention;

[0049] Figure 3 is a healing state curve provided by an embodiment of the present invention;

[0050] In the figure: 1. Grafting preparation module; 2. Status detection module; 3. Evaluation module;

[0051] Figure 4 Schematic diagram of a wearable sensor provided by an embodiment of the invention. DETAILED DESCRIPTION

[0052] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0053] The present invention solves the technical problem of the difficulty in achieving real-time, non-destructive monitoring in the prior art by using wearable sensors to judge the healing state of melon grafted seedlings. In traditional monitoring of grafted seedlings, the use of contact sensors or manual observation methods can easily cause physical damage to the plants, and it is impossible to continuously obtain data on the healing state of the grafted seedlings. The present invention achieves non-destructive and highly sensitive continuous monitoring through the application of flexible wearable sensors, avoiding interference with the grafted seedlings.

[0054] In addition, the existing technology of grafted seedling healing status monitoring usually requires complex equipment or time-consuming experimental operations, which is inefficient and cannot meet the automation requirements in modern agricultural production. The present invention adopts impedance measurement technology and directly attaches the sensor to the scion, which has a high degree of automation and can quickly and accurately feedback the healing status of the grafted part, greatly improving the detection efficiency and real-time performance, and meeting the needs of industrial applications.

[0055] In terms of industrial application, the technology of the present invention has high flexibility and adaptability, and can be widely used in different plant grafting scenarios in agricultural production, especially in large-scale seedling cultivation and grafting operations. Its non-contact and non-destructive characteristics effectively reduce the loss of grafted seedlings, improve the survival rate of plants, reduce production costs, and provide a new solution for the intelligentization of modern agriculture.

[0056] In summary, while solving the problems of contact monitoring damage, complex operation, and delayed data acquisition in traditional technologies, the present invention has achieved significant technological progress in the growth monitoring of grafted plants through flexible sensors and automated monitoring methods, and promoted technological innovation and industrial application of precision agriculture.

[0057] like Figure 1 As shown, an embodiment of the present invention provides a non-destructive monitoring method for judging the healing state of a melon grafted seedling using a wearable sensor, the method comprising:

[0058] 1) Preparation of flexible stretchable adhesive layer;

[0059] First, prepare a cleaned wafer as a substrate. The cleaned substrate needs to be dried in an oven. In order to remove the PDMS from the substrate at the end, a sacrificial layer needs to be applied to the substrate. The sacrificial layer can be applied by spin coating using materials such as photoresist. Then, a layer of polydimethylsiloxane (PDMS) mixture is spin-coated on the surface and heated to cure to form a flexible stretchable adhesion layer, which is finally separated and removed from the wafer;

[0060] 2) Preparation of carbon precursor material layer;

[0061] The flexible stretchable adhesive layer is firstly subjected to plasma cleaning treatment, and then a layer of polyimide is spin-coated on the flexible stretchable adhesive layer, and cured at 200° C. for 2 hours to form a carbon precursor material layer;

[0062] 3) Preparation of conductive material layer:

[0063] Using a laser to carve on the carbon precursor material layer, under the action of the laser, the carbon precursor material layer is graphitized, and a pre-designed interdigital electrode pattern can be carved to form a conductive material layer;

[0064] 4) Preparation of porous material functional layer;

[0065] The conductive material layer is immersed in a solution containing graphene oxide, or spin coating, spray coating, drop coating, etc. The substrate coated with the GO solution is placed in a drying oven for drying to remove the solvent and solidify the graphene oxide layer, and finally a porous material functional layer is formed on the surface.

[0066] The specific steps include:

[0067] S1, pumpkin rootstock and melon scion prepared for grafting;

[0068] S2, grafting is done when the first true leaf of pumpkin rootstock is fully expanded and the first true leaf of scion is half expanded;

[0069] S3, attaching the sensor to the cotyledons of the scion to continuously detect the growth status of the grafted seedling;

[0070] S4, the impedance signal of the sensor is measured by the instrument, and the impedance change curve 10 days after grafting is drawn to evaluate the grafting healing status.

[0071] In the S1, the pumpkin rootstock for grafting is prepared, including: disinfecting the pumpkin rootstock seeds with 1‰ potassium permanganate solution for 10 minutes, washing with tap water for 3 times, and then soaking the seeds in 55-60°C warm water for 6-8 hours; then placing the seeds in a culture dish covered with moist gauze and covering with plastic wrap to keep moisture, placing in a 30°C incubator for germination for 24 hours, sowing in a 50-hole plug tray when the seeds are white, and grafting when the first true leaf is fully unfolded.

[0072] In the S1, preparing the melon scion for grafting includes: disinfecting the melon scion seeds with 1‰ potassium permanganate solution for 10 minutes, washing with tap water for 3 times, and then soaking the seeds in 55-60°C warm water for 6-8 hours; then placing the seeds in a culture dish covered with moist gauze and covering with plastic wrap to keep moisture, placing in a 30°C incubator for germination for 24 hours, sowing in a 100-hole tray when the seeds are white, and grafting when the first true leaf is half-expanded.

[0073] The S2 includes:

[0074] Stock processing includes: using a blade to cut off the true leaves, growth points and one cotyledon at an angle of 45 degrees from the base of the cotyledon, leaving only one cotyledon; keeping the cut surface at 0.5 cm long, and completing it in one cut as much as possible to ensure a smooth cut surface;

[0075] The scion processing includes: using a blade to make an oblique cut at 45 degrees upwards at about 1.0 cm from the base of the cotyledons, and the cut surface is kept at 0.5-1.0 cm and is smooth.

[0076] Water the substrate thoroughly one day before grafting, and sterilize the blades, grafting clips and grafting healing environment used for grafting with 75% medical alcohol in advance, and use them after natural drying.

[0077] In S3, the requirement for the grafted seedlings is: continuous monitoring of a single plant, and approximately 10 grafted seedlings need to be monitored to complete the healing state curve.

[0078] In S3, first, during the graft healing period, the grafted seedlings are placed in a small, closed environment with high humidity. When collecting data, we need to transfer the grafted seedlings to an environment with environmental parameters of humidity of 55%-60% and temperature of 23°C-26°C, and collect data after 3-4 minutes.

[0079] The data collection process is as follows: first, the electrodes are attached to the surface of the scion leaves, and then the timing is started. After 1 minute, the data is almost stable, and the data is recorded at this time; first, several grafted seedlings are prepared. After grafting, the day of grafting is day 0, and the leaves of the scion are monitored and data is collected from the first day.

[0080] like Figure 3 As shown, in S4, the graft healing state is evaluated by the inflection point of the descending curve: the earlier the inflection point, the better the graft healing state.

[0081] like Figure 2 As shown, the present invention provides a non-destructive monitoring system for determining the healing state of a melon grafted seedling, which implements the non-destructive monitoring method for determining the healing state of a melon grafted seedling. The system comprises:

[0082] Grafting preparation module 1, preparing pumpkin rootstock and melon scion for grafting; grafting is performed when the first true leaf of the pumpkin rootstock is completely flattened and the first true leaf of the scion is half-expanded;

[0083] The state detection module 2 is connected to the grafting preparation module 1, and the sensor is attached to the cotyledons of the scion to continuously detect the growth state of the grafted seedling;

[0084] The evaluation module 3 is connected to the state detection module 2, measures the impedance signal of the sensor through the instrument, and draws the impedance change curve 10 days after grafting to evaluate the grafting healing state.

[0085] The working principle of this non-destructive monitoring method is based on impedance sensing technology, which determines the healing status of the grafted seedlings by measuring the changes in the impedance signal of the grafted seedlings.

[0086] 1. Grafting Preparation and Operation

[0087] Before grafting, choose the right pumpkin rootstock and melon scion and make sure they are in good growth condition. Grafting should be done when the first true leaf of the pumpkin rootstock is fully expanded and the first true leaf of the melon scion is half expanded. This time point helps to improve the success rate of grafting and the healing effect.

[0088] 2. Sensor attachment and signal acquisition

[0089] After the grafting is completed, the impedance sensor is attached to the cotyledon of the scion. The sensor monitors the growth status of the grafted seedling in real time by detecting the bioelectric changes in the grafting site and its surroundings. The sensor reflects biological information such as the moisture content and tissue healing status of the grafting site through impedance changes.

[0090] 3. Impedance signal measurement and analysis

[0091] After grafting, the sensor continuously measures the impedance signal on the scion leaves and transmits the data to the connected instrument. The instrument converts the impedance signal into a curve and draws the impedance change curve of the grafted seedling within 10 days by analyzing the change trend of the curve. The change of impedance signal is closely related to the cell activity, water content and tissue healing process during the healing process, and can reflect the different stages of graft healing.

[0092] 4. Evaluation of healing status

[0093] By looking at the changing trend of the impedance signal curve, researchers can determine the healing status of the grafted seedlings. During the graft healing process, the impedance signal will fluctuate with the change of cell activity. The stability, growth or fluctuation of the signal can reveal the health status and healing progress of the grafted site. This can quickly and non-destructively determine the healing degree of the grafted seedlings and provide timely feedback on the graft quality.

[0094] This method realizes non-destructive monitoring of grafted seedlings, has the characteristics of high efficiency and continuous detection, and can significantly improve the scientificity and accuracy of grafted seedling management.

[0095] The following are two specific embodiments of the method for non-destructively monitoring the healing state of melon grafted seedlings based on the wearable sensor of the present invention:

[0096] Embodiment 1:

[0097] Sensor preparation and grafting monitoring

[0098] 1. Preparation of flexible and stretchable adhesion layer: Use the cleaned wafer substrate, spin-coat photoresist as a sacrificial layer, and bake at 120°C for 15 minutes. Then, spin-coat the PDMS mixture on the substrate and put it in an 80°C oven for curing for 1 hour to form a flexible and stretchable adhesion layer.

[0099] 2. Preparation of carbon precursor material layer: Use plasma to clean the surface of the flexible adhesion layer, then spin-coat polyimide on the adhesion layer, put it into an oven and cure it at 200° C. for 2 hours to form a carbon precursor material layer.

[0100] 3. Preparation of the conductive material layer: A laser engraving system is used to carve an interdigitated electrode pattern on the carbon precursor material layer. The polyimide layer is partially graphitized under the action of high-energy laser to form a conductive carbon material layer.

[0101] 4. Preparation of porous material functional layer: Immerse the prepared sensor in graphene oxide solution, coat the surface of the conductive layer with a porous graphene oxide layer, and dry and solidify it.

[0102] 5. Grafting monitoring: Select pumpkin rootstock and melon scion, and graft when the first true leaf of the scion is half-expanded. Attach the prepared sensor to the cotyledon of the scion, connect it to the impedance measuring instrument, monitor continuously for 10 days after grafting, and record the impedance signal changes to finally evaluate the graft healing status.

[0103] Embodiment 2:

[0104] Improved sensors and data processing

[0105] 1. Preparation of flexible layer: As in Example 1, a PDMS flexible layer was first prepared, but the thickness of the PDMS was adjusted to 200 μm during spin coating to increase the flexibility of the sensor.

[0106] 2. Preparation of carbon precursor material layer and conductive material layer: After plasma cleaning the adhesion layer, polyimide is spin-coated, and an ultra-fine laser engraving system is used to design the electrode pattern, ultimately generating a finer conductive material layer to improve the sensitivity of the sensor.

[0107] 3. Improvement of the porous functional layer: Use a higher concentration of graphene oxide solution for coating, combined with microwave drying method, to improve the conductivity and stability of the porous layer.

[0108] 4. Grafting monitoring and data processing: During the grafting process of pumpkin rootstock and melon scion, the sensor is attached to the cotyledon of the scion, and the embedded data processing module is used to record the impedance change data after grafting in real time. The monitoring data is transmitted to the cloud via wireless, and real-time analysis and impedance change curve are drawn. After 10 days, a healing status report is output to accurately evaluate the grafting effect.

[0109] Through these two embodiments, the operability and high-precision advantages of the present invention in practical applications can be demonstrated.

[0110] The above description is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto. Any modifications, equivalent substitutions and improvements made by any technician familiar with the technical field within the technical scope disclosed by the present invention and within the spirit and principle of the present invention should be covered by the protection scope of the present invention.

Claims

1. A non-destructive monitoring method for judging the healing state of melon grafted seedlings using a wearable sensor, characterized in that: The method includes: S1, preparing a flexible stretchable adhesive layer: coating a sacrificial layer on a cleaned and dried wafer substrate, coating a polydimethylsiloxane (PDMS) mixed solution by spin coating, and heating and curing, and then peeling off to obtain a flexible stretchable adhesive layer; S2, preparing a carbon precursor material layer: plasma cleaning the flexible stretchable adhesion layer, then spin coating polyimide thereon, and curing at 200° C. for 2 hours to form a carbon precursor material layer; S3, preparing a conductive material layer: laser engraving a carbon precursor material layer to partially graphitize it, forming a preset interdigital electrode pattern to form a conductive material layer; S4, preparing a porous material functional layer: soaking or coating the conductive material layer with a graphene oxide (GO) solution, drying and curing the graphene oxide layer to form a porous material functional layer; S5, grafting and monitoring: Attach the sensor to the cotyledons of the melon scion, connect the instrument to measure the impedance signal, draw the impedance change curve 10 days after grafting, and evaluate the graft healing status based on the impedance change.

2. The nondestructive monitoring method for determining the healing state of melon grafted seedlings using a wearable sensor according to claim 1, characterized in that: In the S1, the pumpkin rootstock for grafting is prepared, including: disinfecting the pumpkin rootstock seeds with 1‰ potassium permanganate solution for 10 minutes, washing with tap water for 3 times, and then soaking the seeds in 55-60°C warm water for 6-8 hours; then placing the seeds in a culture dish covered with moist gauze and covering with plastic wrap to keep moisture, placing in a 30°C incubator for germination for 24 hours, sowing in a 50-hole plug tray when the seeds are white, and grafting when the first true leaf is fully unfolded.

3. The non-destructive monitoring method for determining the healing state of melon grafted seedlings using a wearable sensor according to claim 1, characterized in that: In the S1, preparing the melon scion for grafting includes: disinfecting the melon scion seeds with 1‰ potassium permanganate solution for 10 minutes, washing with tap water for 3 times, and then soaking the seeds in 55-60°C warm water for 6-8 hours; then placing the seeds in a culture dish covered with moist gauze and covering with plastic wrap to keep moisture, placing in a 30°C incubator for germination for 24 hours, sowing in a 100-hole tray when the seeds are white, and grafting when the first true leaf is half-expanded.

4. The nondestructive monitoring method for determining the healing state of melon grafted seedlings using a wearable sensor according to claim 1, characterized in that: The S2 includes: Stock processing includes: using a blade to cut off the true leaves, growth points and one cotyledon at an angle of 45 degrees from the base of the cotyledon, leaving only one cotyledon; keeping the cut surface at 0.5 cm long, and completing it in one cut as much as possible to ensure a smooth cut surface; The scion processing includes: using a blade to make an oblique cut at 45 degrees upwards at about 1.0 cm from the base of the cotyledons, and the cut surface is kept at 0.5-1.0 cm and is smooth.

5. The non-destructive monitoring method for determining the healing state of melon grafted seedlings using a wearable sensor according to claim 1, characterized in that: Water the substrate thoroughly one day before grafting, and sterilize the blades, grafting clips and grafting healing environment used for grafting with 75% medical alcohol in advance, and use them after natural drying.

6. The nondestructive monitoring method for determining the healing status of melon grafted seedlings using a wearable sensor according to claim 1, characterized in that: In S3, the requirement for the grafted seedlings is: continuous monitoring of a single plant is required, and approximately 10 grafted seedlings need to be monitored to complete the healing state curve.

7. The non-destructive monitoring method for determining the healing state of melon grafted seedlings using a wearable sensor according to claim 1, characterized in that: In S3, first, during the graft healing period, the grafted seedlings are placed in a small, closed environment with high humidity. When collecting data, we need to transfer the grafted seedlings to an environment with environmental parameters of humidity of 55%-60% and temperature of 23°C-26°C, and collect data after 3-4 minutes. The data collection process is as follows: first, the electrodes are attached to the surface of the scion leaves, and then the timing is started. After 1 minute, the data is almost stable, and the data is recorded at this time; first, several grafted seedlings are prepared. After grafting, the day of grafting is day 0, and the leaves of the scion are monitored and data is collected from the first day.

8. The non-destructive monitoring method for determining the healing status of melon grafted seedlings using a wearable sensor according to claim 1, characterized in that: In S4, the graft healing state is evaluated by the inflection point of the descending curve: the earlier the inflection point, the better the graft healing state.

9. A nondestructive monitoring system for judging the healing state of a grafted melon seedling using a wearable sensor, which implements the nondestructive monitoring method for judging the healing state of a grafted melon seedling using a wearable sensor as claimed in any one of claims 1 to 8, characterized in that: The system includes: The preparation of the wearable sensor is composed of four layers of materials stacked in sequence from the outside to the inside:

1. Flexible stretchable adhesion layer; 2. Carbon precursor material layer; 3. Conductive material layer; 4. Porous material functional layer. The flexible stretchable adhesion layer is a polydimethylsiloxane layer, the carbon precursor material layer is a polyimide layer, the conductive material layer is a graphene layer, and the porous material functional layer is a graphene oxide layer. The grafting preparation module prepares pumpkin rootstock and melon scion for grafting; grafting is performed when the first true leaf of the pumpkin rootstock is completely flattened and the first true leaf of the scion is half-opened; The state detection module is connected to the grafting preparation module, and the sensor is attached to the cotyledons of the scion to continuously detect the growth state of the grafted seedling; The evaluation module is connected to the status detection module, measures the impedance signal of the sensor through the instrument, and draws the impedance change curve 10 days after grafting to evaluate the grafting healing status.

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