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Bacillus amyloliquefaciens for preventing and treating botrytis cinerea in plants and application of bacillus amyloliquefaciens

A technology for dissolving starch spores and plant ash, applied in the direction of chemicals for biological control, methods based on microorganisms, applications, etc., to achieve sustained and efficient antagonism and good biological control effects

Inactive Publication Date: 2017-11-10
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Although many kinds of bacillus have been developed for the preparation of microbial fungicides, due to the diversity of bacillus populations, that is, strains, different bacillus and even different strains of the same bacillus have different antibacterial spectrum, antibacterial activity and antibacterial characteristics. Screening highly effective Bacillus biocontrol agents with different targets is still a topic of concern for scientific research in various countries now and for a long time to come.

Method used

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  • Bacillus amyloliquefaciens for preventing and treating botrytis cinerea in plants and application of bacillus amyloliquefaciens
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  • Bacillus amyloliquefaciens for preventing and treating botrytis cinerea in plants and application of bacillus amyloliquefaciens

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Embodiment Construction

[0042] The present invention will be described in detail below in combination with specific embodiments.

[0043]The Bacillus amyloliquefaciens used for the prevention and treatment of botrytis cinerea related to the present invention was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on June 29, 2017, and the address is No. 1, Beichen West Road, Chaoyang District, Beijing, No. 3 No., the deposit name is Bacillus amyloliquefaciens sw17 (Bacillus amyloliquefaciens sw17), and the deposit number is CGMCC No.: 14365.

[0044] 1. Isolation, screening and identification of Bacillus amyloliquefaciens sw17:

[0045] (1) Separation and screening:

[0046] From the demonstration apple orchard in Luochuan County, Yan'an City, Shaanxi Province, the healthy apple rhizosphere soil was collected and brought back in fresh-keeping bags;

[0047] Weigh 10g of soil sample, put it into a conical flask filled with 90mL of sterile wat...

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Abstract

The invention relates to bacill us amyloliquefaciens sw17 for preventing and treating botrytis cinerea in plants and an application of the bacillus amyloliquefaciens. The bacterium (the bacillus amyloliquefaciens sw17) is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No.14365; and the bacterium can take an inhibitory effect on gray mold of strawberry, gray mold of tomato and gray mold of cucumber caused by botrytis cinerea Pers., with a bacteriostasis rate ranging from 62% to 74%. The bacillus amyloliquefaciens sw17, which is provided by the invention is separated from rhizosphere soil of healthy fruit trees in a demonstration orchard, is free from ecological toxicity, high in safety and good in antibacterial effect, and the bacillus amyloliquefaciens sw17 is simple in culture and short in cycle; and the bacillus amyloliquefaciens sw17 has a quite broad application prospect in biological control of plant diseases.

Description

technical field [0001] The invention relates to the field of development and utilization of microbial germplasm resources, in particular to a bacillus amyloliquefaciens used for preventing and treating botrytis cinerea and its application. Background technique [0002] Botrytis cinerea, also known as gray rot and white spot, is one of the main diseases in crop production, mostly caused by Botrytis cinerea Pers. Botrytis cinerea is a fungus of the genus Botrytis, and its sexual form is Botryotiniafuckeliana Whetzel. It is not common in the natural state and can cause gray mold of more than 200 dicotyledonous plants, including solanum Solanaceae (Tomato, Capsicum annuum Linn., etc.), Cucurbitaceae (Cucumis sativus Linn., Cucurbita pepoLinn., etc.), Rosaceae (Strawberry, Begonia ( Malus spectabilis Borkh.), etc.], grape family (Vitaceae), etc., can also cause broad bean (Vicia faba Linn.) red spot, flower rot, British rot, etc. More than 1400 hosts have recently been reported...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 马赛箭薛文娇安超上官亦卿丁浩
Owner MICROBIOLOGY INST OF SHAANXI
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