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Set of methods suitable for evaluating toxicity changes of pesticide residues in soil

A pesticide residue and soil technology, which is applied in the field of evaluating changes in the toxicity of pesticide residues in soil, can solve the problems of incomplete and inaccurate determination of the residual toxicity, and the degradation products cannot be extracted or detected, so as to facilitate genotoxicity and ecotoxicity Measurement, wide applicability, and reproducible effects

Inactive Publication Date: 2014-12-10
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

This is because: (1) The state of pesticides in the soil is a dynamic process of degradation and transformation, accompanied by the generation of degradation intermediates, which will inevitably lead to different impacts on the environment; (2) A pesticide often produces multiple Degradation products, and the polar nature of the degradation products is not necessarily the same as that of the parent pesticide. For example, endosulfan is a fat-soluble pesticide, but one of its degradation products, endosulfan sulfate, is a water-soluble organic compound. If only based on the properties of the parent pesticide, It will inevitably lead to insufficient comprehensiveness in the determination of its residual toxicity; (3) when the degradation products of a certain pesticide are measured by experimental instruments, what is detected is its main degradation products, and some very small amounts of degradation products cannot be extracted or detected. It is inaccurate to use the toxicity of several degradation products to judge the toxicity changes of pesticides after degradation

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  • Set of methods suitable for evaluating toxicity changes of pesticide residues in soil
  • Set of methods suitable for evaluating toxicity changes of pesticide residues in soil
  • Set of methods suitable for evaluating toxicity changes of pesticide residues in soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 determines residual toxicity extraction method process

[0030] Organic solvent extraction method

[0031] Preliminary experiment scheme: Take 20g of test soil sample and use petroleum ether: acetone (volume ratio is 1: 1) mixed solution 120mL, first soak in Soxhlet extractor for 12h and then extract for 6h. The extract was concentrated to about 1mL on a rotary evaporator to obtain a soil extract; Note: when the amount is small, add DMSO 2-3 times, 1ml each time, and continue to blow.), use DMSO (dimethyl sulfoxide) solvent to make the volume to 5mL, and use it to prepare for the test.

[0032] According to this experimental plan, if all the earthworms used in the experiment die, the experimental method should be modified. The revision mainly solves the following three questions: (1) whether dimethyl sulfoxide (DMSO) is toxic to earthworms, and whether DMSO can be used to fix the volume; (2) the mixture of petroleum ether and acetone (volume ratio 1:1 ) ...

Embodiment 2

[0084] Comparison of different property pesticide residue toxicity determination methods in the soil of embodiment 2

[0085] 1. Test soil

[0086]After the soil was ground, it passed through a 2mm sieve, and four pesticides with different properties were selected as representatives and added to the soil at a certain concentration. These four kinds of pesticides are respectively endosulfan, acetamiprid, imidacloprid and atrazine, and the concentration of each pesticide in the soil is set as follows: the concentration of endosulfan in the soil is set as: control group (0), 0.1 , 1.0, 10.0mg / kg four treatments; adding acetamiprid to the soil set the concentration as: control group (0), 0.05, 0.1, 0.5mg / kg four treatments; adding imidacloprid to the soil set the concentration as: control Group (0), 0.1, 0.5, 1mg / kg four treatments; adding atrazine to the soil set the concentration as: control group (0), 0.1, 0.5, 2.5mg / kg four treatments. After the pollutants are added to the s...

Embodiment 3

[0143] Embodiment 3 Application of the present invention in endosulfan degradation dynamic process

[0144] Centrifuge the cultured strains in a low-temperature refrigerated centrifuge at 8000rpm for 8min at 15°C. After the centrifugation, pour off the supernatant and collect the sediment at the bottom of the centrifuge tube, that is, the wet bacteria. Dissolve the wet bacteria in 20mL (pH 7.0 ) of 0.05mol / L phosphate buffer (Na 2 HPO 4 -NaH 2 PO 4 ) to wash the components 3 times, centrifuge again under the above conditions for 8min, and then use 0.05mol·L -1 Phosphate buffer solution and wet bacteria were prepared by vortexing and mixing at a mass ratio of 3:1 to prepare endosulfan-degrading bacteria suspension. Get 40 brown bottles of 125ml, be divided into 2 test groups: one group adds endosulfan to soil, another group adds endosulfan and endosulfan degrading bacteria to soil, each group respectively establishes 20 brown bottles, two test groups Add 20g of soil to the...

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Abstract

The invention relates to a set of methods suitable for evaluating toxicity changes of pesticide residues in soil. According to different properties of the pesticide residues in the soil, the set of methods comprises an organic solvent extraction method, a deionized water extraction method and a slurry direct exposure infection method respectively; through an earthworm comet assay and a broad bean micronucleus test, soil ecotoxicity is detected on the basis of double indexes; the set of methods suitable for evaluating the toxicity changes of the pesticide residues in the soil is established, thereby providing a technical support for evaluating influence of toxicities of the pesticide residues on the environment and human health. The set of methods is simple and feasible, high in repeatability and wide in applicability and can be applied to pesticides with different polar properties; by the set of methods, the subsequent determination of the ecotoxicity is facilitated; the set of methods has a relatively great practical application value.

Description

technical field [0001] The invention relates to a set of methods for evaluating the toxicity change of pesticide residues in soil. technical background [0002] It is well known that pesticides play a significant role in ensuring and promoting the development of agriculture, forestry and animal husbandry, and satisfying people's needs for agricultural and sideline products. However, the serious situation of pesticide pollution has attracted more and more attention. In recent years, due to the massive and unreasonable application of pesticides, farmland soil has been polluted. This not only destroys the biodiversity in the soil and causes serious harm to the soil ecological environment, but also enters the human body through drinking water or through the soil plant system through the food chain, endangering human health. [0003] The pollutant concentration of pesticide residues is low and the range is wide. At present, there are few domestic and foreign scholars studying t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/24
Inventor 朱鲁生孔玲芬王军王金花谢慧
Owner SHANDONG AGRICULTURAL UNIVERSITY
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