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Method for detecting acute biological toxicity of sewage

A technology for biological toxicity and detection methods, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, and testing water. It can solve the problems of underestimated toxicity, unstable test results, and poor repeatability, and achieve the effect of reducing interference.

Inactive Publication Date: 2011-09-07
XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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Problems solved by technology

[0003] However, direct luminescent bacteria toxicity tests on natural water samples often result in unstable test results and poor reproducibility due to the existence of some "non-toxic" coexisting substances in the water that can stimulate luminescent bacteria to luminesce and grow.
For example for NH 3 -N or PO 4 -Water bodies with high P content may underestimate the toxicity of some substances due to its ability to stimulate bacteria to luminescence or serve as bacteria nutrients to cover up the toxicity of various organic pollutants, which may lead to large errors in test results and poor repeatability
In addition, the existing methods do not clearly stipulate the specific amount of samples and strains used in the detection process, resulting in uncertainty in the detection results, and it is difficult to express quantitatively

Method used

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  • Method for detecting acute biological toxicity of sewage

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[0032] The present invention improves the sample detection system by pre-treating sewage samples, and provides an accurate detection method for acute biological toxicity evaluation of polluted water, and uses freshwater luminescent bacteria—Vibrio Qinghai Q67, which can make the detection results more stable and detect The reproducibility has been greatly improved. The method specifically includes the following steps:

[0033] Step 1, sample processing:

[0034] Sewage samples were taken according to the standard sampling method, and the samples were treated as follows:

[0035] (1) Pass 7 mL of dichloromethane, 7 mL of methanol, and 7 mL of pure water through the C18 solid-phase extraction column in sequence to activate the column by wetting, and do not allow the filler to be drained in the middle;

[0036] (2) Take 500 mL of sewage sample filtered by a 0.45 μm filter membrane and put it on the column, make the sewage sample continuously pass through the C18 solid-phase ext...

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Abstract

The invention specifically relates to a method for detecting acute biological toxicity of sewage, belonging to the field of environmental water detection. A method for accurately detecting the acute biological toxicity of a sewage sample can be established by the following steps of: extracting and concentrating organic matter in a sewage sample, removing the interference of inorganic matter, and finally detecting by using fresh water luminous bacteria Qinghai vibrio Q67. The method can provide accurate final results, is stable and has good reproducibility. The method is the effective improvement of the traditional method.

Description

technical field [0001] The invention belongs to the field of detection methods for environmental water bodies, and in particular relates to a detection method for acute biological toxicity of sewage. Background technique [0002] The detection methods of acute biological toxicity of sewage mainly include: fish acute toxicity test, daphnia toxicity test, algae toxicity test, microbial toxicity test, etc. Among them, the microbial toxicity test mostly uses the luminescent bacteria method, which has been widely used because of its short detection time, low cost, and low requirements for experimental conditions and test sites. [0003] However, direct testing of the toxicity of luminescent bacteria on natural water samples often results in unstable test results and poor reproducibility due to the existence of some "non-toxic" coexisting substances in the water that can stimulate the luminescence and growth of luminescent bacteria. For example for NH 3 -N or PO 4 -Water bodies...

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Application Information

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IPC IPC(8): G01N21/00G01N33/18C12Q1/18
Inventor 刘永军马晓妍王晓昌张爱宁
Owner XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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