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Acyl-homoserine lactone compound and application thereof in environmental protection

A technology for acylation of homoserine and ester compounds, applied in the field of environmental microorganisms, can solve problems such as biological toxicity, secondary pollution, and drug resistance of acidophilic Thiobacillus ferrooxidans in mining areas, and achieve the purpose of inhibiting the production of acidic heavy metal mine water , no secondary pollution, good inhibitory effect

Active Publication Date: 2016-11-23
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The most commonly used method is the chemical method, which is to prevent the pollution of acid mine water by putting in bacteriostatic agents or bactericides to inhibit the activity of leaching bacteria. However, this method cannot be used as a long-term control method
The reason is: the regeneration of bacteria makes people have to continuously add these fungicides or antibacterial agents, which will not only lead to the emergence of drug resistance of acidophilic Thiobacillus ferrooxidans, but also produce toxicity to the water organisms in the mining area, causing secondary pollution

Method used

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  • Acyl-homoserine lactone compound and application thereof in environmental protection
  • Acyl-homoserine lactone compound and application thereof in environmental protection
  • Acyl-homoserine lactone compound and application thereof in environmental protection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Synthesis of embodiment 1 Thiobacillus ferrooxidans biofilm inhibitor

[0032]

[0033] In the formula,

[0034] No.1: R 1 =Br,R 2 = H, R 3 = H, n = 2;

[0035] No.2: R 1 =Br,R 2 = H, R 3 =H,n=0;

[0036] No. 3: R 1 = H, R 2 = H, R 3 =Br,n=0;

[0037] No. 4: R 1 = H, R 2 =Br,R 3 =H,n=0;

[0038] No.5: R 1 = Cl, R 2 = H, R 3 =H,n=0;

[0039] No. 6: R 1 = H, R 2 = F, R 3 =H,n=0;

[0040] No. 7: R 1 = F, R 2 = H, R 3 =H,n=0;

[0041] No. 8: R 1 = NO 2 , R 2 = H, R 3 =H,n=0;

[0042] No. 9: R 1 = CF 3 , R 2 = H, R 3 =H,n=0;

[0043] No. 10: R 1 =Br,R 2 = H, R 3 = H, n = 1;

[0044] Among them, No.1, No.2, No.3, No.4, No.5, No.6, No.7, No.8, No.9, and No.10 are respectively a kind of acylated homoserine lactone Compound analogues.

[0045] Concrete synthetic steps are:

[0046] (1) Place 169 mg (0.93 mM) of (R)-(+)-α-amino-γ-butyrolactone hydrochloride and the corresponding 0.93 mM organic acid in 10 mL of dry CH 2 Cl 2Mix well...

Embodiment 2

[0048] The influence of embodiment 2 compound on the normal growth of Thiobacillus ferrooxidans

[0049] Experimental materials: A. ferrooxidans ATCC23270 was purchased from American Type Culture Collection (ATCC).

[0050] Acidophilus ferrooxidans converts Fe 2+ Oxidized to Fe 3+ Gain energy to grow. Detection of Fe in Solution by Potassium Dichromate Titration 3+ content, reflecting the effect of compounds on bacterial Fe 2+ Oxidation ability, so as to ensure that the drug concentration will not affect the normal growth of bacteria. The experimental method is as follows:

[0051] (1) Add 100mL of 9K medium with pH 1.8 into a 250mL Erlenmeyer flask (9K medium preparation method: mix 3.0g (NH 4 ) SO 4 , 0.1g KCl, 0.5g K 2 HPO 4 , 0.5g MgSO 4 ·7H 2 O, 0.01g Ca(NO 3 ) 2 , 44.67g FeSO 4 ·7H 2 O was dissolved in 1000mL distilled water, and the pH was adjusted to 2.0 with dilute sulfuric acid, and sterilized by filtration), and then the experiment was divided into th...

Embodiment 3

[0055] Example 3 Biofilm Inhibitor Inhibition Activity Detection

[0056] (1) Collect A.ferrooxidans ATCC23270 cells by centrifuging at 10°C for 15 min at 9000 r / min, wash the cells twice with dilute sulfuric acid solution with pH 2.0, and finally resuspend them in a solution containing 2 g / L Fe 2+ , pH 2.0 basic salt solution (without FeSO 4 9K medium), and the bacterial density of the bacterial suspension was adjusted to 1.0×10 8 cells / mL;

[0057] (2) Divide the above-mentioned bacterial suspension into three groups, the first group uses no compound as the blank control group; compound. The bacterial suspension was added to a 96-well cell culture plate, 150 μL / well, and 8 parallels were made for each experimental group. Then put the 96-well cell culture plate into a constant temperature incubator, and culture it at 30°C for 48 hours to form a biofilm;

[0058] (3) After 48 hours, discard the bacteria solution in the wells, and wash each well twice with sterile deionize...

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Abstract

As existence of acidophilic thiobacillus ferrooxidans on surface of metal sulfide ore in the form of a bio-membrane, dissolution-out of the metal is greatly improved, thereby forming acidic pit water in high concentration of heavy metal ions and further causing serious environment pollution. The invention discloses a compound which can inhibit formation of bio-membrane of the acidophilic thiobacillus ferrooxidans. The compound, without influence on normal growth of the acidophilic thiobacillus ferrooxidans, can inhibit the formation of the bio-membrane of the acidophilic thiobacillus ferrooxidans, thus reducing the erosion speed of the acidophilic thiobacillus ferrooxidans on the metal sulfide ore and significantly reducing generation of acidic heavy metal pit water in a sulfide mining area. Compared with a bactericide or an antibacterial agent for treating acidic heavy metal pit water pollution, the ecologic toxicity on environment of the compound is reduced to minimum during the treatment on the acidic heavy metal pit water pollution. The acidophilic thiobacillus ferrooxidans is free of drug resistance caused by the bactericide or the antibacterial agent.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a compound for inhibiting the biofilm formation of Thiobacillus ferrooxidans and its application in reducing the production of acidic heavy metal pit water. Background technique [0002] Acid mine drainage (AMD) is mainly formed by a series of physical and chemical reactions such as leaching, oxidation, and hydrolysis of metal sulfide ores under the action of air, water, and microorganisms. At present, the pollution of acid mine water has become one of the most serious environmental problems in the world. Acid mine water has extremely low pH (pH ≤ 2.0) and high concentration of heavy metal ions, so it will cause serious damage to the ecological environment around the mining area. pollute. Acidithiobacillus ferrooxidans (A.ferrooxidans) is one of the most common leaching bacteria in acidic pit water environment. When A.ferrooxidans exists in the ...

Claims

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Application Information

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IPC IPC(8): C07D207/273A01N43/08A01P15/00
CPCA01N43/08C07D207/273
Inventor 李红玉赵阳刘映前
Owner LANZHOU UNIVERSITY
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