Bacillus subtilis bacterial strain Xi-55 and its preparing method

A Bacillus subtilis, xi-55 technology, applied in the direction of bacteria, etc., can solve the problems of difficult cultivation and preparation, unsuitable acquisition, etc., and achieve the effects of no ecotoxicity, good safety, short fermentation period, and easy industrial production.

Inactive Publication Date: 2005-02-16
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the biopesticide varieties, more than 90% are microbial insecticides, and there are relatively few microbial fungicides, especially those that have an inhibitory effect on rice blast fungus, are not suitable for obtaining, and are difficult to cultivate and prepare

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Bacillus subtilis (Bacillus subtilis) strain Xi-55 was obtained through a series of tests of isolation, screening and confrontation of various bacillus in the deep original soil of Xichang City. The specific separation method is: take 10g of the original soil, add 100ml of water, let it stand for 1h, and dilute the concentration of the soil liquid to 10 -5 、10 -6 、10 -7 、10 -8 , and then spread it on the surface of the sterilized KB medium in a zigzag pattern. After culturing for 36-48 hours at a temperature of 28-30°C, pick a single colony and inoculate it on a KB medium plate for culture. The confrontation test was carried out between rice blast fungus and single colony bacteria. After repeated tests, a Bacillus subtikis strain with significant antibacterial effect on rice blast fungus was obtained, which was named Xi-55.

[0023] Preservation method of the strain Xi-55 strain: short-term preservation adopts KB slant medium to preserve the strain. Long-term storag...

Embodiment 2

[0029] The preparation method of this example is:

[0030] a. Propagation method: inoculate the strain on a plate of KB medium, and culture it in a biochemical incubator at 31°C for 43 hours;

[0031] b. Fermentation method: Inoculate the propagated strains in sterilized KB liquid medium for shaking culture, control the temperature at 30°C, rotate at 130r / min, and ferment for 48 hours.

[0032] The KB liquid medium is sterilized by damp heat at 140°C for 20 minutes.

Embodiment 3

[0034] The preparation method of this example is:

[0035] a. Propagation method: inoculate the strain on a plate of KB medium, and culture it in a biochemical incubator at 27°C for 53 hours;

[0036] b. Fermentation method: Inoculate the propagated strains in sterilized KB liquid medium for shaking culture, the temperature is controlled at 29°C, the rotation speed is 130r / min, and the fermentation time is 50h.

[0037] The KB liquid medium is sterilized by moist heat at 100°C for 20 minutes.

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PUM

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Abstract

The invention publishes a kind of dull grass Bacillu subtilis atrain, Xi-55(CGMCCNo.1070), and its preparation method. The strain can well restrain murrain germ in paddy. used to prevent and cure paddy murrain, it has no pollution and no residual and better security. Its preparation is easier, the fermentation cycle is shorter, cost is lower and it is easier to be stored. Thus, it is more convenient for agriculture.

Description

Technical field [0001] The invention relates to a bacillus subtilis strain and a preparation method thereof. Background technique [0002] In order to control chemical pollution and ensure food safety, developed countries such as the United States and Europe have taken the development of ecological agriculture, reducing the use of chemical pesticides, research and development of biological pesticides as the main development direction of plant pest control. Biological control is generally accepted and highly valued in the integrated pest management system for its advantages of no pollution, no residue, no ecotoxicity and good safety, among which microbial pesticides play a leading role in the biological control system. Since the 1990s, the global production of biological pesticides has been increasing at a rate of 10%-20% every year. At present, there are more than 100 kinds of biopesticide products in the world, including more than 10 kinds of biotechnology products. Among...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 彭化贤刘波微刘秀芳
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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