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Method for detecting ecotoxicity effect quantification of low-dose polluted soil

A technology of polluted soil and ecotoxicity, applied in the field of pollution detection, can solve the problems of difficult determination by chemical methods, no clear dose-effect relationship of earthworm avoidance reaction, chemical method underestimates the effective toxicity of pollutants, etc., and achieves the effect of shortening the identification time

Inactive Publication Date: 2010-12-08
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

However, purely using chemical methods to detect soil health has great limitations; first, chemical methods are difficult to comprehensively measure various substances in soil, and it is impossible to identify the potential toxic effects of all substances in soil
Second, chemical methods tend to underestimate the effective toxicity of pollutants: Third, chemical methods cannot accurately evaluate the toxicity of pollutants in a quantitative manner
[0003] After searching the literature, it was found that Chinese patents "A method and experimental device for rapid detection of soil health quality (public number: CN1469122A)", "a device and method for rapid evaluation of contaminated soil remediation effect (public number: CN101231277)" and "Earthworm Avoidance Toxicity Test Kit (Publication Number: CN201083730)" are both designed based on the principle of earthworm avoidance response. A major deficiency of these documents is that there is no clear dose-effect relationship of earthworm avoidance response. Therefore, it is difficult to truly quantitatively detect pollution Soil toxicity also affects the application of the method

Method used

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  • Method for detecting ecotoxicity effect quantification of low-dose polluted soil
  • Method for detecting ecotoxicity effect quantification of low-dose polluted soil
  • Method for detecting ecotoxicity effect quantification of low-dose polluted soil

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Toxic effects of cadmium-contaminated soil detected by open avoidance tank

[0027] The first step is to make the detection device. The detection device is made of plexiglass and has a cylindrical structure. First, draw a middle marking line on the outer wall of the cylinder, and divide it into 6 avoidance tanks evenly with impermeable partitions.

[0028] The second step: prepare poisoned soil. CdCl 2 2.5H 2 After conversion, O is calculated as pure cadmium, dissolved in deionized water to prepare mother liquor, and the mother liquor dilution method is used to prepare six concentrations of 0, 50, 100, 200, 400, 600mg / kg lower than EC 50 The test solution, each test solution volume is 100ml. Add the above test solution to 1kg of soil, mix well, and place it under natural conditions indoors for 24 hours. And adjust the soil to 60% of its maximum water holding capacity with distilled water, after stirring evenly, add 300g of wet soil into the detection device in a ra...

Embodiment 2

[0035] Detection of Ecotoxicity Effect of Low Dose of Cadmium Contaminated Soil in Semi-open Avoidance Tank

[0036] The first step is to manufacture the detection device. The detection device is made of plexiglass and has a cylindrical structure. First, draw a middle marking line on the outer wall of the cylinder, and divide it into 6 avoidance tanks evenly with a semi-permeable partition with three small holes at the bottom.

[0037] The second step: prepare poisoned soil. CdCl 2 2.5H 2 After conversion, O is calculated as pure cadmium, dissolved in deionized water to prepare mother liquor, and the mother liquor dilution method is used to prepare six concentrations of 0, 50, 100, 200, 400, 600mg / kg lower than EC 50 The test solution, each test solution volume is 100ml. Add the above test solution to 1kg of soil, mix well, and place it under natural conditions indoors for 24 hours. And adjust the soil to 60% of its maximum water holding capacity with distilled water, aft...

Embodiment 3

[0044] Ecotoxicity Effect of Low Dose Phenanthrene Contaminated Soil by Semi-open Avoidance Trough

[0045] Step 1: Fabrication of the detection device. The detection device is made of plexiglass and has a cylindrical structure. First, draw a middle marking line on the outer wall of the cylinder, and divide it into 6 avoidance tanks evenly with a semi-permeable partition with three small holes at the bottom.

[0046] The second step: prepare poisoned soil. Dissolve phenanthrene in acetone to prepare mother liquor, and use mother liquor dilution method to prepare six concentrations of 0, 10, 20, 30, 40, 50 mg / kg lower than EC according to the concentration ratio relationship 50 The test solution, each test solution volume is 100ml. Add the above test solution to 1kg of soil, mix well, and place it under natural conditions indoors for 24 hours. And use distilled water to adjust the soil to 60% of its maximum water holding capacity. After stirring evenly, add 300g of wet soil ...

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Abstract

The invention discloses a method for detecting the ecotoxicity effect quantification of low-dose polluted soil, comprising the following steps of: averagely dividing a detection device into six avoiding grooves by using waterproof partition boards; preparing contaminated soil and adding the contaminated soil in the detection device; equivalently adding 10-20 soil test animals in each avoiding groove and cultivating at constant temperature; calculating the avoiding percentage of earthworms in the soil; establishing the dose-reaction relationship between different concentrations of contaminants and the earthworm avoiding percentage by using a linear model fitting method and preparing a standard curve; and placing the field adopted contaminated soil in one of the avoiding grooves, calculating the avoiding reaction of the soil test animals and calculating the concentration of the contaminant. The invention has the advantages of simple operation, low cost, easy observation, short test period, high reaction sensitivity, relevance of a test ending point with the ecology and the like, is not only suitable for repairing and detecting the contaminated soil, but also can be used for carrying out quick risk screening and detection on the contaminated soil in emergency or sudden events.

Description

technical field [0001] The invention relates to a method for detecting pollution in the technical field of detection, in particular to a method for quantitatively detecting ecotoxicity effects of low-dose polluted soil. Background technique [0002] Soil is an indispensable and important public resource for economic and social development, which is related to the quality and safety of agricultural products and human health. Toxicity monitoring of contaminated soil is an essential and important link in the process of soil cleaning. However, there are great limitations in simply using chemical methods to detect soil health status. First, chemical methods are difficult to comprehensively measure various substances in soil, and it is impossible to identify the potential toxic effects of all substances in soil. Second, chemical methods tend to underestimate the effective toxicity of pollutants; third, chemical methods cannot accurately evaluate the toxicity of pollutants in a qu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/24
Inventor 沈国清余月书姜官鑫朱鸿林
Owner SHANGHAI JIAO TONG UNIV
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