Process for extracting litchi polyphenol from litchi rind

A technology of litchi polyphenols and litchi skin, which is applied in the field of extraction of active ingredients in plants, can solve the problems of low yield of litchi polyphenols, certain requirements for extraction equipment, high production cost, etc., to improve water solubility and bioavailability , to achieve effective protection and avoid the effect of oxidation

Inactive Publication Date: 2014-07-09
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another example is the invention patent with the publication number CN102895388A, which discloses a kind of lychee peel is extracted with lye as a solvent, and then the extract is acidified, and the substance formed by the reaction of polyphenol and alkali is reduced to polyphenol, which overcomes the prior art. The use of organic solvents as solvents has the disadvantages of high production costs and low polyphenol content in the extract, but the solvent used in this method is relatively alkaline, which requires certain requirements for extraction equipment. At the same time, the yield of litchi polyphenols in this method Low, about 0.7-0.8% (fresh lychee skin)
The above-mentioned inventions all failed to take overall consideration and realization of the protection of polyphenols, the degradation of polymers (improve bioavailability), and the high yield of litchi polyphenol products.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Take 3 kg of fresh lychee peel, cut it into flakes, soak it in 18 liters of deionized water with pH=3 (adjust the pH with hydrochloric acid), extract at 60°C, degrade for 5 hours, filter, and repeat the extraction of the filter residue under the same conditions for 1 times, filter, and combine the filtrates to obtain the extract;

[0021] 2) The obtained extract was subjected to D-101 macroporous adsorption resin chromatography, the resin column was washed with water until the effluent was clear, and then gradient eluted with ethanol with a volume concentration of 20-70%, followed by thin-layer chromatography for detection, and the collected Alcohol eluent of target components;

[0022] 3) After recovering ethanol from the collected ethanol eluate under reduced pressure, vacuum drying was performed to obtain 37.8 g of litchi polyphenol extract. The polyphenol content was 86.6% and the yield was 1.09% as detected by F-C method.

[0023] Take 1 gram of the product pre...

Embodiment 2

[0025] 1) Take 3.2kg of fresh lychee peel, cut it into slices, soak it in 20 liters of methanol with pH=5 (adjust the pH with sulfuric acid), extract at 90°C, degrade for 5 hours, filter, and repeat the extraction of the filter residue once under the same conditions , filter, and combine the filtrates to obtain an extract;

[0026] 2) The obtained extract is subjected to AB-8 macroporous adsorption resin chromatography, the resin column is washed with water until the effluent is clear, and then gradient eluted with methanol with a volume concentration of 20 to 70%, followed by thin-layer chromatography for detection, and the collection containing Alcohol eluent of target components;

[0027] 3) After recovering the methanol from the collected ethanol eluent under reduced pressure, vacuum drying was performed to obtain 39.6 g of litchi polyphenol extract. The polyphenol content was 85.3% and the yield was 1.06% as detected by F-C method.

[0028] Get 1 gram of the product prep...

Embodiment 3

[0030] 1) Take 3.0 kg of dried litchi peel, crush it, soak it in 25 liters of deionized water with pH=5 (adjust the pH with hydrochloric acid), extract at 60°C, degrade for 4 hours, filter, and repeat the extraction of the filter residue once under the same conditions. Filter and combine the filtrates to obtain the extract;

[0031] 2) The obtained extract is subjected to HPD300 macroporous adsorption resin chromatography, the resin column is first washed with water until the effluent is clear, and then gradient eluted with ethanol with a volume concentration of 10-80%, followed by thin-layer chromatography to collect the target component Alcohol eluent;

[0032] 3) After recovering ethanol from the collected ethanol eluate under reduced pressure, vacuum drying was performed to obtain 58.2 g of litchi polyphenol extract. The polyphenol content was 86.7% and the yield was 1.68% as detected by F-C method.

[0033] Get 1 gram of the product prepared in this example and place it ...

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PUM

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Abstract

The invention discloses a process for extracting litchi polyphenol from litchi rind. The process comprises the following steps: regulating the pH of an extracting solvent to be less than or equal to 6.0 with acid; extracting and degrading the litchi rind with the pH value-regulated extracting solvent; allowing the extract to pass through a macroporous adsorption resin column; washing the column with water, and then with low-carbon alcohol; tracing and checking with thin layer chromatography, and collecting alcohol eluent containing a target component; and concentrating and drying the alcohol eluent. According to the preparation method, the litchi rind is extracted by adopting the acidity-regulated extracting solvent, on the one hand, the litchi polyphenol is effectively protected, the oxidation degree of polyphenol substances in the extracting process can be furthest avoided, and on the other hand, the whole system is in an acidic condition, so that the polymer in the polyphenol substances can be degraded into small-molecular substances with good water solubility, and the water solubility and bioavailability of the obtained product can be effectively improved. Furthermore, the process is simple and easy to operate, and the product is high in polyphenol content and high in yield.

Description

technical field [0001] The invention relates to a method for extracting active components in plants, in particular to a process for extracting litchi polyphenols from litchi skin. Background technique [0002] Litchi (Litchi chinensis) is a Sapindaceae (Sapindaceae) plant, and its main production areas are the southwest and south of my country, and it is a famous fruit. Due to the concentrated production period of lychee, strong seasonality, and difficulty in keeping fresh, the deep processing of the product presents the characteristics of short time and heavy tasks. A large number of by-products of lychee peel produced during the processing are usually treated as waste, which has not been well treated. Make full use of it as the lengthening of the litchi industry chain and the improvement of the industry level. Litchi skin is rich in polyphenols, mainly epicatechin, rutin, ferulic acid, etc. Litchi polyphenols have biological activities such as anti-oxidation, anti-inflamm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/77A23L1/30A61K131/00
Inventor 黄永林李典鹏卢凤来刘金磊陈月圆杨子明颜小捷王磊
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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