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Process for extracting and purifying punicosides

A pomegranate polyphenol and process technology, which is applied in the field of extraction and purification of pomegranate polyphenols, can solve the problems of damage, unfavorable separation and purification of polyphenols, and high impurity content in an extract, so as to improve extraction efficiency, reduce losses, and reduce solvents. the effect of using

Active Publication Date: 2013-08-14
SHANGHAI TONGJI BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The extraction of pomegranate polyphenols and the utilization of pomegranate peels (seeds) are mostly decocting and ethanol reflux extraction methods, which are likely to cause damage to polyphenols and other antioxidant components in pomegranate peels (seeds), and The high content of impurities is not conducive to the separation and purification of polyphenols

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A process for extracting and purifying pomegranate polyphenols is as follows:

[0030] The first step, raw material crushing: after drying the fresh pomegranate peel, use a hammer mill to crush through a 10-mesh sieve for later use;

[0031] The second step, ultrasonic extraction: use 40% ethanol solution as the extraction agent, the ratio of solid to liquid is 1:15, the ultrasonic working frequency is 40kHz, the extraction temperature is 60°C, the extraction time is 30 minutes, and the extraction is performed 3 times;

[0032] The third step, filtering: filter the feed liquid with a filter of 80 mesh stainless steel coarse filter, the filtration flow rate is 500ml / min, the filtration pressure is 0.25Mpa, the temperature of the filtrate is 55°C, and the filtrate is collected and put into a storage tank for subsequent use;

[0033] The fourth step, chromatographic separation and purification: pump the filtrate into the chromatographic column, and then use the gradient el...

Embodiment 2

[0039] A process for extracting and purifying pomegranate polyphenols is as follows:

[0040] The first step, raw material crushing: after drying the fresh pomegranate peel, use a hammer mill to crush through a 10-mesh sieve for later use;

[0041] The second step, ultrasonic extraction: use 40% ethanol solution as the extraction agent, the ratio of solid to liquid is 1:15, the ultrasonic working frequency is 40kHz, the extraction temperature is 60°C, the extraction time is 40 minutes, and the extraction is performed 3 times;

[0042] The third step, filtering: the feed liquid is filtered with a filter of 80 mesh stainless steel coarse screens, the filtration flow rate is 550ml / min, the filtration pressure is 0.30Mpa, the temperature of the filtrate is 40°C, and the filtrate is collected and put into a storage tank for subsequent use;

[0043] The fourth step, chromatographic separation and purification: pump the filtrate into the chromatographic column, and then use the gradi...

Embodiment 3

[0049] A process for extracting and purifying pomegranate polyphenols is as follows:

[0050] The first step is to crush the raw materials: pass the pomegranate seeds through 20 meshes, and esterify them with petroleum ether for later use;

[0051] The second step, ultrasonic extraction: use 40% ethanol solution as the extraction agent, the ratio of solid to liquid is 1:15, the ultrasonic working frequency is 40kHz, the extraction temperature is 60°C, the extraction time is 35 minutes, and the extraction is performed 3 times;

[0052] The third step, filtering: the feed liquid is filtered with a filter of 80 mesh stainless steel coarse filter, the filtration flow rate is 520ml / min, the filtration pressure is 0.28Mpa, the temperature of the filtrate is 50°C, and the filtrate is collected and put into a storage tank for subsequent use;

[0053]The fourth step, chromatographic separation and purification: pump the filtrate into the chromatographic column, and then use the gradien...

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Abstract

The invention provides a process for extracting and purifying punicosides, which comprises the following steps of: (1), crushing raw materials; (2), carrying out ultrasonic extraction for three times, wherein 40% of ethanol solution taken as an extraction agent; the solid-to-liquid ratio is 1:15; the ultrasonic working frequency is 40kHz; the extraction temperature is 60 DEG C; and the extraction time is 30-40 min; (3), filtering; (4), carrying out chromatographic separation and purification, wherein a mobile phase is taken as alcoholic solution; the concentration is 55-65%; the flow velocity is 50-60 ml / min; and the pressure is 0.25-0.30MPa; (5), concentrating in vacuum; and (6), freezing and drying in vacuum, wherein the thickness is 2-2.5cm; the freezing temperature is minus 40 DEG C; the drying time is 10-12h; and the vacuum degree is minus 0.8 MPa. According to the process, by comprehensively applying diversified technologies, the loss of effective components is well reduced; the extraction efficiency of the punicosides is greatly improved and the polyphenol content is greatly increased (the extraction efficiency of the punicosides is up to 34-40%, and the polyphenol content is up to 55-90%); simultaneously, energy is saved; and the use of solvents is reduced.

Description

technical field [0001] The invention belongs to the technical field of health food, and relates to an extraction and purification process of pomegranate polyphenols. Background technique [0002] The traditional extraction method of pomegranate peel (seed) is incompletely extracted by solvent extraction, which not only increases the production cost, but more importantly, affects the efficiency; the solvent consumption is large, and both water decoction and solvent extraction methods require More water resources and organic solvents. And inevitably cause more energy consumption such as electricity, fuel, etc.; active ingredients may cause losses during the extraction process. In addition, it has the disadvantages of low efficiency and poor reproducibility, and the reagents used are usually poisonous, which is easy to cause harm to the environment and operators. And it is easy to cause damage to polyphenols and other antioxidant components in pomegranate peel (seed), and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61P35/00A61K8/97A61P39/06A23L1/29A61K131/00A61K36/185A23L33/00
Inventor 桂仁斌王云华马福全
Owner SHANGHAI TONGJI BIOLOGICAL PROD
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