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Water chestnut shell polyphenol extract as well as preparation method and application thereof

A technology for husk polyphenols and extracts, applied in the biological field, can solve problems such as reports of polyphenol extraction, and achieve the effects of high polyphenol content, low cost and simple process

Inactive Publication Date: 2015-04-29
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Water chestnut shell contains a large amount of polyphenolic compounds, and there is no report on the extraction of polyphenols in water chestnut shell

Method used

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  • Water chestnut shell polyphenol extract as well as preparation method and application thereof
  • Water chestnut shell polyphenol extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Dry and pulverize the water chestnut husk, weigh 0.5 kg of water chestnut husk powder, add 20 L of ethanol with a concentration of 50%, heat reflux extraction for 3 times, heat reflux extraction for 1 hour each time, and combine the extracts. The extract is concentrated under reduced pressure to an extract, which is dissolved in water 10 times the weight of the extract, and filtered to remove insoluble matter to obtain a loading solution. Take 0.25kg D101 macroporous resin (non-polar macroporous resin column), load the sample solution, elute with 5L of water, discard the eluent, and then elute with 5L of 10% ethanol. The 10% ethanol eluate was collected, concentrated and dried under reduced pressure to obtain polyphenol extract from water chestnut husk.

[0030] 5.3g sample was obtained by this method, and the polyphenol content of the extract was measured to be 57.3% by ultraviolet spectrophotometry. It has been determined that the polyphenol extract has a certain inh...

Embodiment 2

[0032]The water chestnut husk was dried and crushed, weighed 0.5 kg of water chestnut husk powder, added 50 L of methanol with a concentration of 80%, extracted by hot reflux for 3 times, each time for 1 hour, and combined the extracts. The extract was concentrated under reduced pressure to an extract, which was dissolved in 50% ethanol 40 times the weight of the extract, and filtered to remove insoluble matter to obtain a loading solution. Take 5.0kg of HPD722 type macroporous resin (weakly polar macroporous resin column), load the sample solution, elute with 5L of water, discard the eluent, and then elute with 5L of 30% ethanol. Collect 30% ethanol eluate, concentrate and dry under reduced pressure to obtain polyphenol extract from water chestnut husk.

[0033] 10.1g sample was obtained by this method, and the polyphenol content of the extract was measured to be 82.9% by UV spectrophotometry. It has been determined that the polyphenol extract has a certain inhibitory effect...

Embodiment 3

[0035] The water chestnut husk was dried and pulverized, weighed 0.5 kg water chestnut husk powder, added 5 L of 80% ethanol, dipped and extracted twice, each time for 15 days, and combined the extracts. The extract was concentrated under reduced pressure to an extract, which was dissolved in 10% ethanol 10 times the weight of the extract, and centrifuged to remove insoluble matter to obtain a loading solution. Take 2.0kg LS-305 macroporous resin (weakly polar macroporous resin column), load the sample solution, elute with 5L water, discard the eluent, and then elute with 4L 50% ethanol. Collect 40% ethanol eluate, concentrate and dry under reduced pressure to obtain polyphenol extract from water chestnut husk.

[0036] 16.7g sample was obtained by this method, and the polyphenol content of the extract was determined to be 80.2% by UV spectrophotometry. It has been determined that the polyphenol extract has a certain inhibitory effect on the activity of α-glucosidase. When th...

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Abstract

The invention discloses a water chestnut shell polyphenol extract as well as a preparation method and application thereof. The preparation method comprises the following step: by taking water chestnut shells as raw materials, performing alcohol extraction and macroporous resin purification processes to prepare the water chestnut shell polyphenol extract. The water chestnut shell polyphenol extract prepared by using the method disclosed by the invention has significant alpha-glucosidase inhibition effects, and can be further researched and developed to be taken as a health product or a medicine for adjuvant treatment of diabetes.

Description

technical field [0001] The invention specifically relates to a water chestnut shell polyphenol extract and a preparation method and application thereof, belonging to the field of biotechnology. Background technique [0002] α-glucosidase specifically catalyzes the hydrolysis of sucrose or maltose containing α-glucoside, and α-glucosidase inhibitors can inhibit the activity of α-glucosidase in the upper small intestine and block the breakdown of carbohydrates into single glucose, thereby Lower postprandial blood sugar in diabetic patients. As a unique oral hypoglycemic drug, α-glucosidase inhibitors can improve glucose metabolism disorders and delay or prevent the occurrence of chronic vascular complications of diabetes for patients with type 2 diabetes who are not satisfied with diet control and exercise therapy . [0003] Water chestnut is the fruit of the annual aquatic plant water chestnut of the genus Lingaceae, and is widely planted in freshwater waters in my country...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P3/10A23L1/29A23L33/00
Inventor 吕寒马丽陈剑李维林任冰如简暾昱梁呈元刘艳
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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