Extraction method of soil microbe genome DNA and total RNA

A technology of soil microorganisms and extraction methods, used in recombinant DNA technology, DNA preparation, etc.
CN101974513AInactive Publication Date: 2011-02-16FUJIAN AGRI & FORESTRY UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
FUJIAN AGRI & FORESTRY UNIV
Publication Date
2011-02-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for effectively extracting crop rhizosphere soil microbe genome DNA and total RNA, which comprises the following steps: firstly, removing humus and other impurities which severely disturb nucleic acid extraction in soil samples by aluminum sulfate; further crushing soil microbe cells by glass beads; adding sodium dodecyl sulfonate (SDS) and LiCl solutions for cracking cells and dissociating nucleic acids; adding the mixture of phenol, chloroform and isoamylol for extracting; and then, depositing the extraction solution by isopropanol and sodium acetate to finally acquire the soil microbe metagenome DNA and total RNA. The method is suitable for extraction of the DNA and total RNA of soil in different regions. The invention establishes an extraction technology which has the advantages of simple and easy operation processes and higher purity of the prepared sample, and can provide an important basis for the research of soil metagenomics.
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Description

technical field

[0001] The invention relates to a method for extracting soil microbial genome DNA and total RNA, belonging to the field of biotechnology. technical background

[0002] Due to the limitations of traditional microbiological research methods and other factors, the development and utilization of microbial resources as the source of life science research and the basis of biotechnology innovation once fell into a stalemate of high-frequency repeated screening, and the continuous development of modern biotechnology has gradually overcome these difficulties. . In the study of modern soil microecology, the genomic DNA of microorganisms in the soil is directly extracted, and on this basis, current advanced molecular biology techniques are used, such as amplified fragment length polymorphism (AFLP) technology, terminal restriction fragment length polymorphism T-RFLP technology, single-strand configuration polymorphism (SSCP), denaturation / temperature gradient gel elect...

Claims

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