The disclosure relates to multilayered vesicles, methods for forming multilayered vesicles, and
drug delivery complexes including multilayered vesicles. The multilayered vesicles can be formed by adding
hydrophobe containing polypeptoid (HCP)-lipid complexes to unilamellar vesicles such as liposomes in an amount effective to form multilayered vesicles. The HCP-lipid complexes can be produced by mixing a
hydrophobe containing polypeptoid (HCP) with a first set of unilamellar vesicles. The unilamellar vesicles and / or
drug delivery complexes can include various cargoes for delivery, such as water-soluble cargoes, hydrophobic cargoes, etc.