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Method for ultrasonic extracting and resin purifying sweetening agent in Lithocarpus polysachyus rehd leaf

A technology of ultrasonic wave and sweetener, which is applied in the field of ultrasonic extraction and resin purification of sweeteners in the leaves of Cote abacus, which can solve the problems of unfavorable formulation of quality standards for sweet tea products and less sweeteners

Inactive Publication Date: 2014-06-04
HUNAN AOKANG BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As a kind of natural sugar plant, the research on sweeteners of Polyacaceae is relatively few. At present, most of them are purification and determination of total flavonoids or single components in Polyacaceae, which is not conducive to the quality of sweet tea and other products. Standard formulation

Method used

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  • Method for ultrasonic extracting and resin purifying sweetening agent in Lithocarpus polysachyus rehd leaf
  • Method for ultrasonic extracting and resin purifying sweetening agent in Lithocarpus polysachyus rehd leaf
  • Method for ultrasonic extracting and resin purifying sweetening agent in Lithocarpus polysachyus rehd leaf

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Embodiment 1

[0021] 1 Materials and methods

[0022] 1.1 Materials

[0023] The leaves of Lithocarpus litseifolius (Hance) Chun were collected in Zhijiang County, Hunan Province, and identified as the leaves of Lithocarpus litseifolius (Hance) Chun.

[0024] 1.2 Reagents and instruments and extraction methods

[0025] AB-8 type macroporous resin Tianjin Haiguang Chemical Co., Ltd.; Phloridin and Neohesperidin Dihydrochalcone (NHDC) reference substance (>99%) Sigma Company; Chromatography Methanol Tianjin Kemiou Chemical Reagent Co., Ltd.; Pure water from Huaihua Wahaha Hengfeng Beverage Co., Ltd.; ethanol, hydrochloric acid, sodium hydroxide, sodium dihydrogen phosphate, etc. were of analytical grade.

[0026] CTXNW-10B Ultrasonic Cycle Extractor Beijing Hongxianglong Biotechnology Development Co., Ltd.; RE-210 Rotary Evaporator Shanghai Yarong Biochemical Instrument Factory; LC-20A High Performance Liquid Chromatograph Japan Shimadzu Bio-Instrument Co., Ltd.; AL104 Electronic Balance US...

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Abstract

The invention discloses a method for ultrasonic extracting and resin purifying sweetening agent in Lithocarpus polysachyus rehd leaf. The optimal ultrasonic extracting method is as follows: the ethanol concentration is 70%, the solid to liquid ratio is 1:25, and the ultrasonic time is 35min; the optimal AB-8 resin purifying process condition is as follows: the adsorption flow rate is 0.5mL / min, the elution ethanol concentration is 90%, the elution volume is 1.875BV, and the flow rate of the eluant is 1Ml / min. Under above extracting and purifying condition, the purity of the obtained phlorizin is 88.6164%, the purity of the neohesperidin dihydrochlcone is 71.8226%. The extracting and purifying method is simple and reliable and in favor of utilizing the sweetening agent in Lithocarpus polysachyus rehd leaf on scale.

Description

technical field [0001] The invention belongs to the technical field of natural medicines, and in particular relates to a method for ultrasonically extracting and resin-purifying sweeteners in Ke leaves. Background technique [0002] Lithocarpus litseifolius (Hance) Chun is an evergreen plant of the genus Lithocarpus litseifolius (Fagaceae). It is called sweet tea because of its high sweetness after soaking its leaves. Kedo multispike is distributed in the provinces south of the Yangtze River in a wild state. Among them, Hunan, Fujian, Jiangxi, Guangxi, Anhui and other provinces are rich in resources and concentrated, and Hunan is more distributed in the Xuefeng Mountain area. According to the "National Chinese Herbal Medicine Compilation" and other records: Duosuike tea can prevent and treat high blood pressure, treat damp-heat dysentery, skin itching, carbuncle and malignant sores, etc. In addition, sweet tea has anti-inflammatory, analgesic, immune regulation, enhanced an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H1/08A23L1/236A23L27/30
Inventor 李爱民李胜华伍贤进
Owner HUNAN AOKANG BIOTECHNOLOGY CO LTD
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