The invention discloses an osmanthus fragrans flavor yeast polypeptide colloidal particle feed and a preparation method thereof. The feed comprises the following raw materials by weight part: 70-90 of wall-breaking yeast powder, 4-5 of pea powder, 3-5 of red date flesh powder, 1-2 of mushroom protein, 0.01-0.03 of protease, 0.01-0.02 of lipase, 2-3 of Osmanthus yunnanensis nectar, 1-2 of mashed banana, 1-2 of coconut milk, 0.3-0.6 of a bitter orange extract, 2-3 of konjac gum, 2-3 of sodium carboxymethyl starch, 2-3 of pumpkin starch, and 1-2 of soybean oligosaccharides. The feed provided by the invention has faint scent and sweet taste, is in the shaped of colloidal particles, has soft texture and comfortable and refreshing taste, is easy to be accepted by animals, and can significantly improve the feed intake of animals, reduce the stress response of animals, promote animal growth and weight gain, and improve animal reproductive performance.