Pericarpium citri reticulatae compound protein powder as well as preparation method and application thereof

Abstract

The invention discloses pericarpium citri reticulatae compound protein powder as well as a preparation method and application thereof. The protein powder takes pericarpium citri reticulatae extract microcapsules, seville orange flower extract microcapsules, soybean protein isolate powder, polydextrose, resistant dextrin, soybean peptide powder, tremella powder and the like as main components, and further comprises edible salt, edible essence, a nutrition enhancer, a sweetening agent and an anti-caking agent. The preparation method comprises the steps of raw material weighing, premixing, high-speed shearing and mixing and the like. Active components of the dried orange peel and seville orange flower extract are extracted through an ultrasonic-assisted ethanol extraction method, and sodium alginate and chitosan are adopted for microencapsulation treatment, so that the stability and solubility of the dried orange peel and seville orange flower extract are improved. The compound protein powder disclosed by the invention has the functions of maintaining muscle mass and relieving symptoms such as nausea and abdominal distension in a weight loss process, has good storage stability and safety, and is suitable for being taken for a long time.

Description

Technical Field

[0001] The present invention relates to the technical field of functional foods, and particularly relates to a tangerine peel composite protein powder and its preparation method and application. Background Art

[0002] With the continuous improvement of people's attention to health and body management, weight loss and body shaping have become the goals pursued by many people. However, although methods such as drug weight loss and gastrointestinal surgery weight loss can achieve weight loss to a certain extent, they are often accompanied by gastrointestinal discomfort symptoms such as nausea and abdominal distension, as well as skeletal muscle reduction, seriously affecting the quality of life and weight loss experience of patients. Tangerine peel and bitter orange flower, as traditional Chinese medicinal materials with both medicinal and edible uses, have the effects of regulating qi and strengthening the spleen, soothing the liver and regulating qi, and reducing adverse qi and stopping vomiting, and can effectively relieve the above-mentioned discomfort symptoms. Combining processed tangerine peel and bitter orange flower with protein powder can not only provide high-quality protein to maintain muscle mass, but also relieve common gastrointestinal discomfort during weight loss, and has important application value. Summary of the Invention

[0003] The present invention aims to provide a tangerine peel composite protein powder and its preparation method and application, and to prepare a functional protein powder that can not only maintain muscle mass but also relieve symptoms such as nausea and abdominal distension during weight loss.

[0004] To achieve the above object, the present invention provides the following technical solution: a tangerine peel composite protein powder and its preparation method and application. This composite protein powder is prepared by extracting the active ingredients from processed tangerine peel and bitter orange flower, and combining them with high-quality protein powder and other nutritional components to prepare a functional protein powder that can not only maintain muscle mass but also relieve symptoms such as nausea and abdominal distension during weight loss.

[0005] The tangerine peel composite protein powder of the present invention, calculated by mass fraction, includes: 5 - 10 parts of tangerine peel extract microcapsules; 3 - 7 parts of bitter orange flower extract microcapsules; 40 - 50 parts of soy protein isolate; 10 - 20 parts of polydextrose; 8 - 12 parts of resistant dextrin; 6 - 10 parts of soy peptide powder; 5 - 8 parts of tremella powder; 1 - 2 parts of edible salt; 0 - 2 parts of edible essence; 1 - 3 parts of nutritional fortifier; 0.05 - 0.1 part of sweetener; 1 - 2 parts of anticaking agent.

[0006] Preferably, the tangerine peel composite protein powder is preferably calculated by mass fraction as: 8 parts of tangerine peel extract microcapsules; 5 parts of bitter orange flower extract microcapsules; 45 parts of soy protein isolate; 15 parts of polydextrose; 10 parts of resistant dextrin; 8 parts of soy peptide powder; 6.5 parts of tremella powder; 1.5 parts of edible salt; 1 part of edible essence; 2 parts of nutritional fortifier; 0.08 part of stevioside; 1.5 parts of silicon dioxide.

[0007] Preferably, the nutritional fortifier is at least one of concentrated milk protein powder, calcium caseinate, and microencapsulated flaxseed oil powder. The nutritional fortifier is preferably in the following parts by mass: 0.6 part of concentrated milk protein powder, 0.6 part of calcium caseinate, and 0.8 part of microencapsulated flaxseed oil powder.

[0008] Specifically, the preparation of tangerine peel extract:

[0009] S1. Process the tangerine peel: Select dry and mature tangerine peel, remove impurities, and cut it into small pieces; put the tangerine peel pieces into a pot, add an appropriate amount of water, boil over low heat and continue to cook for 10 - 15 minutes, then take it out and let it dry; put the dried tangerine peel pieces into an oven and dry at 60°C - 70°C until the water content is less than 10%, to obtain the processed tangerine peel.

[0010] S2. Crush the processed tangerine peel, and use ultrasonic-assisted ethanol extraction method to extract the active ingredients in the tangerine peel, with ultrasonic power of 300W, temperature of 50°C, and time of 30min. After filtration, concentrate to obtain the tangerine peel extract.

[0011] Specifically, the preparation of bitter orange flower extract:

[0012] S1. Process the bitter orange flower: Select dry bitter orange flowers, remove the flower stalks and impurities; put the bitter orange flowers into a pot, add an appropriate amount of water, boil over low heat and continue to cook for 8 - 12 minutes, then take it out and let it dry; put the dried bitter orange flowers into an oven and dry at 60°C - 70°C until the water content is less than 10%, to obtain the processed bitter orange flowers.

[0013] S2. Crush the processed bitter orange flowers, and use ultrasonic-assisted ethanol extraction method to extract the active ingredients in the bitter orange flowers, with ultrasonic power of 300W, temperature of 50°C, and time of 30min. After filtration, concentrate to obtain the bitter orange flower extract.

[0014] Specifically, microencapsulation:

[0015] S1. Dissolve sodium alginate in deionized water to obtain solution A; dissolve chitosan in acetic acid solution to obtain solution B.

[0016] S2. Mix the extract, emulsifier (such as Tween 80) and water evenly to obtain solution C.

[0017] S3. Slowly drip solution C into solution A to obtain an emulsion, and then slowly drip the emulsion into solution B. After the reaction is completed, filter, wash, and dry to obtain the extract microcapsules.

[0018] Specifically, during the microencapsulation process, the concentration of sodium alginate is 2wt.% - 5wt.%, the concentration of chitosan is 1wt.% - 3wt.%, and the concentration of the emulsifier is 0.5wt.% - 2wt.%.

[0019] Specifically, the mass ratio of the tangerine peel extract, sodium alginate, and chitosan is 80:1:1; the mass ratio of the bitter orange extract, sodium alginate, and chitosan is 70:1:1.

[0020] Specifically, the preparation method of the tangerine peel compound protein powder

[0021] S1. Weigh the raw materials according to the ratio;

[0022] S2. Premix the soy protein isolate and resistant dextrin by low-speed shearing;

[0023] S3. Add the tangerine peel extract microcapsule, the bitter orange extract microcapsule, and polydextrose for the first high-speed shearing mixing;

[0024] S4. Finally, add the remaining raw materials for the second high-speed shearing mixing. After all the raw materials are mixed evenly, the tangerine peel compound protein powder is obtained.

[0025] Specifically, the shearing rate of the low-speed shearing premixing is 100 - 500 rpm, the mixing time is 5 - 15 min, and the mixing temperature is 20 - 40 °C; the shearing rate of the first high-speed shearing mixing is 500 - 2000 rpm, the mixing time is 3 - 10 min, and the mixing temperature is 25 - 45 °C; the shearing rate of the second high-speed shearing mixing is 1000 - 3000 rpm, the mixing time is 8 - 15 min, and the mixing temperature is 30 - 50 °C.

[0026] The principle and beneficial effects of this technical solution:

[0027] This tangerine peel compound protein powder extracts and microencapsulates the active ingredients in the processed tangerine peel and bitter orange, combines them with high-quality protein powder and other nutritional components to form a functional protein powder. The ultrasonic-assisted ethanol extraction method is used to efficiently extract the active ingredients of tangerine peel and bitter orange, and then through the microencapsulation process of sodium alginate and chitosan, the extracts are wrapped to form a stable microcapsule structure, protecting the active ingredients from the external environment and at the same time improving the solubility and stability. In the formula, soy protein isolate and soy peptide powder are used as the main protein sources to provide high-quality protein to maintain muscle mass and support body repair; polydextrose and resistant dextrin are used as dietary fibers to promote intestinal health and relieve discomfort symptoms such as abdominal distension during weight loss; tremella powder is rich in polysaccharides, further enhancing intestinal function; nutritional fortifiers supplement additional nutrients to improve the overall nutritional value of the product. The active ingredients of tangerine peel and bitter orange can regulate qi and strengthen the spleen, soothe the liver and regulate qi, and reduce adverse qi and stop vomiting, effectively relieve symptoms such as nausea and abdominal distension during weight loss, and are slowly released through the microencapsulation process to continuously exert their effects.

[0028] Using this protein powder can significantly reduce body weight and body fat percentage, and can effectively assist in weight loss. Muscle mass is effectively maintained during the weight loss process, avoiding muscle loss caused by dieting. At the same time, the nausea and bloating scores are significantly reduced. The extracts of tangerine peel and bitter orange can effectively relieve the common gastrointestinal discomfort symptoms during weight loss and improve the weight loss experience. After being stored for 6 months under different temperature conditions, the active ingredient loss rate of the microencapsulated extract is low, and there is no obvious change in color and odor. The microcapsules have good storage stability and are suitable for long-term storage. The particle size distribution of the microencapsulated extract is uniform, and it can be quickly dispersed in water to form a uniform suspension, improving the solubility and taste of the product. It has good safety and is suitable for long-term use. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a process flow diagram for the preparation of this tangerine peel compound protein powder. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0030] The present invention will be further described in detail below with reference to the drawings and embodiments:

[0031] Example 1:

[0032] Preparation of tangerine peel compound protein powder

[0033] 1. Preparation of extract microcapsules

[0034] 1.1 Preparation of tangerine peel extract: The processed tangerine peel is crushed, and the active ingredients in the tangerine peel are extracted by ultrasonic-assisted ethanol extraction method. The ultrasonic power is 300W, the temperature is 50°C, and the time is 30min. After filtration, it is concentrated to obtain tangerine peel extract;

[0035] 1.2 Preparation of bitter orange extract: The processed bitter orange is crushed, and the active ingredients in the bitter orange are extracted by ultrasonic-assisted ethanol extraction method. The ultrasonic power is 300W, the temperature is 50°C, and the time is 30min. After filtration, it is concentrated to obtain bitter orange extract;

[0036] 1.3 Microencapsulation: 3.5 wt.% of sodium alginate is dissolved in deionized water to obtain solution A; 2 wt.% of chitosan is dissolved in acetic acid solution to obtain solution B. The tangerine peel extract and bitter orange extract are respectively mixed evenly with 1 wt.% of Tween 80 and water to obtain solution C. Solution C is slowly dropped into solution A to obtain an emulsion, and then the emulsion is slowly dropped into solution B. After the reaction is completed, it is filtered, washed, and dried to obtain tangerine peel extract microcapsules and bitter orange extract microcapsules respectively.

[0037] 1.4 Preparation of tangerine peel compound protein powder

[0038] Weigh the following raw materials proportionally (by mass parts): 8 parts of tangerine peel extract microcapsules; 5 parts of bitter orange flower extract microcapsules; 45 parts of soy protein isolate powder; 15 parts of polydextrose; 10 parts of resistant dextrin; 8 parts of soy peptide powder; 6.5 parts of tremella powder; 1.5 parts of edible salt; 1 part of edible essence; 2 parts of nutritional fortifier (including 0.6 part of concentrated milk protein powder, 0.6 part of calcium caseinate, and 0.8 part of flaxseed oil microcapsule powder); 0.08 part of stevioside; 1.5 parts of silicon dioxide.

[0039] Premix the soy protein isolate powder and the resistant dextrin at 30 °C with a shear rate of 300 rpm for low-speed shearing for 10 min;

[0040] Add the tangerine peel extract microcapsules, bitter orange flower extract microcapsules, and polydextrose, and perform high-speed shearing and mixing at 35 °C with a shear rate of 1500 rpm for 5 min;

[0041] Finally, add the remaining raw materials, and perform high-speed shearing and mixing at 40 °C with a shear rate of 2000 rpm for 10 min. After mixing evenly, tangerine peel compound protein powder is obtained.

[0042] 1.5 Test the performance of the extract microcapsules

[0043] (1) Encapsulation efficiency

[0044] Detection method: Use high-performance liquid chromatography (HPLC) to determine the content change of active ingredients in tangerine peel extract and bitter orange flower extract before and after microencapsulation and calculate the encapsulation efficiency.

[0045] Result: The encapsulation efficiency of tangerine peel extract microcapsules is 85%, and the encapsulation efficiency of bitter orange flower extract microcapsules is 82%, indicating that the microencapsulation process of the present invention can effectively encapsulate active ingredients.

[0046] (2) Moisture content

[0047] Detection method: Use Karl Fischer titration to determine the moisture content in the microcapsules.

[0048] Result: The moisture content of tangerine peel extract microcapsules is 3.0%, and the moisture content of bitter orange flower extract microcapsules is 3.2%, indicating that the microcapsules have good drying effects and are suitable for long-term storage.

[0049] (3) Storage stability

[0050] Detection method: Store the prepared microcapsules at 25 °C, 4 °C, and -18 °C for 3 months and 6 months respectively, and regularly detect the content of active ingredients and observe the color and smell of the products.

[0051] Results: After being stored at 25°C for 6 months, the loss rate of the active ingredients of the tangerine peel extract microcapsules was 7.0%, and that of the bitter orange extract microcapsules was 6.5%. After being stored at 4°C and -18°C for 6 months, the loss rate of the active ingredients was less than 5%. During the storage process, there were no obvious changes in the color and smell of the microcapsules, indicating that the microcapsules of the present invention have good storage stability.

[0052] (4) Particle size distribution

[0053] Detection method: The particle size distribution of the microcapsules was determined using a laser particle size analyzer.

[0054] Results: The particle sizes of the tangerine peel extract microcapsules and the bitter orange extract microcapsules were mainly distributed between 10 and 50 μm, and the particle size distribution was uniform, which was beneficial to the dissolution and dispersion of the product.

[0055] (5) Solubility and suspension

[0056] Detection method: 1.0 g of the microcapsules was added to 100 mL of deionized water, and stirred until completely dispersed, and its solubility and suspension were observed.

[0057] Results: Both types of microcapsules had good solubility and suspension, and could be quickly dispersed in water to form a uniform suspension.

[0058] 1.6 Testing of clinical efficacy and safety

[0059] (1) Inclusion and grouping of subjects

[0060] Inclusion criteria: Healthy adults aged 18 - 65 years old, BMI ≥ 30 kg / ㎡, with a need for weight loss or a desire to improve gastrointestinal discomfort symptoms through diet regulation; no severe heart, liver, kidney and other organ diseases, no cognitive impairment, etc.; no history of allergy to protein powder, tangerine peel or bitter orange.

[0061] Grouping: The included subjects were randomly divided into an experimental group and a control group, with 100 cases in each group. All subjects signed an informed consent form and agreed to receive a 12 - week intervention.

[0062] (2) Administration plan

[0063] Experimental group: Take the tangerine peel compound protein powder prepared in Example 1 every day, 10 g each time, twice a day, within 30 minutes after breakfast and dinner respectively. The subjects received diet guidance and exercise advice from a dietitian to maintain a healthy lifestyle.

[0064] Control group: Do not take any functional products, only receive diet guidance and exercise advice from a dietitian, which is the same as that of the experimental group.

[0065] (3) Observation Indicators and Detection Methods

[0066] Body weight and body fat percentage: Regular measurements were taken using a precise weighing scale and a body fat analyzer, and the changes in body weight and body fat percentage were recorded and analyzed.

[0067] Gastrointestinal symptom score: Through questionnaires for the subjects, the symptom scores such as nausea and abdominal distension after each meal were recorded to evaluate the alleviating effect of the tangerine peel compound protein powder.

[0068] Muscle mass: Bioelectrical impedance analysis (BIA) technology was used to measure muscle mass, and the changes in muscle mass during weight loss were compared between the experimental group and the control group.

[0069] Safety assessment: The adverse reactions of the subjects during the intervention were recorded to evaluate the safety of the product.

[0070] (4) Statistical Analysis

[0071] Data analysis was performed using SPSS statistical software.

[0072] Measurement data were expressed as mean ± standard deviation, and independent sample t-tests were used for between-group comparisons.

[0073] Count data were expressed as the number of cases (percentage), and chi-square tests were used for between-group comparisons.

[0074] A two-sided P < 0.05 was considered statistically significant.

[0075] (5) Clinical Trial Results

[0076] Table 1 shows the changes in body weight and body fat percentage of the subjects in this experiment, and Table 2 shows the changes in muscle mass of the subjects in this experiment. From Tables 1 and 2, it can be seen that the body weight and body fat percentage of the subjects in the experimental group decreased significantly, and the decrease was greater than that of the control group. The muscle mass of the subjects in the experimental group was effectively maintained during weight loss, while the muscle mass of the subjects in the control group decreased slightly. This indicates that the tangerine peel compound protein powder can maintain muscle mass while losing weight.

[0077] Table 1 Changes in Body Weight and Body Fat Percentage

[0078]

[0079] Table 2 Changes in Muscle Mass

[0080]

[0081] Table 3 shows the gastrointestinal symptom score table of the subjects in this experiment. From Table 3, it can be seen that the scores of nausea and abdominal distension in the experimental group decreased significantly, indicating that the extracts of tangerine peel and flosaurantii can effectively relieve the common gastrointestinal discomfort symptoms during weight loss. The scores of nausea and abdominal distension in the control group only decreased slightly.

[0082] Table 3 Gastrointestinal symptom score

[0083]

[0084] Table 4 is the safety assessment form for the subjects in this experiment. As can be seen from Table 4, the incidence of adverse reactions in the experimental group was significantly lower than that in the control group, and the adverse reactions were all mild gastric discomfort, indicating that the tangerine peel compound protein powder has good safety.

[0085] Table 4 Safety assessment

[0086]

[0087] In summary, the tangerine peel compound protein powder of the present invention shows significant gastrointestinal protection effect and muscle maintenance effect during weight loss, and no obvious adverse reactions are found, having good safety and application prospects.

[0088] The above are only embodiments of the present invention, and specific technical solutions or common knowledge such as characteristics known to the public are not described in detail here. For those skilled in the art, without departing from the technical solution of the present invention, several modifications and improvements can still be made, and these should also be regarded as the protection scope of the present invention, which will not affect the implementation effect of the present invention and the practicality of the patent. The protection scope required by this application shall be subject to the content of its claims, and the specific implementation manners described in the specification can be used to interpret the content of the claims.

Claims

1. A tangerine peel composite protein powder, characterized in that The invention comprises, by mass, 5 to 10 parts of tangerine peel extract microcapsules; 3 to 7 parts of daisy flower extract microcapsules; 40 to 50 parts of soy protein isolate powder; 10 to 20 parts of polydextrose; 8 to 12 parts of resistant dextrin; 6 to 10 parts of soy peptide powder; 5 to 8 parts of tremella powder; 1 to 2 parts of edible salt; 0 to 2 parts of edible flavor; 1 to 3 parts of nutritional enhancer; 0.05 to 0.1 parts of sweetener; and 1 to 2 parts of anti-caking agent.

2. A tangerine peel composite protein powder according to claim 1, characterized in that: The nutritional enhancer is at least one of concentrated milk protein powder, calcium caseinate, and linseed oil microcapsule powder; preferably, the weight parts are: 0.6 parts of concentrated milk protein powder, 0.6 parts of calcium caseinate, and 0.8 parts of linseed oil microcapsule powder.

3. A tangerine peel composite protein powder according to claim 1, characterized in that: The preparation method of tangerine peel extract microcapsules and daisy flower extract microcapsules comprises the following steps: tangerine peel and daisy flower are processed and crushed respectively, active ingredients are extracted by ultrasonic-assisted ethanol extraction, and the active ingredients are filtered and concentrated to obtain tangerine peel extract and daisy flower extract respectively; and tangerine peel extract and daisy flower extract are microencapsulated respectively.

4. A tangerine peel composite protein powder according to claim 3, characterized in that: The microencapsulation comprises the following steps: S1. Dissolve sodium alginate in deionized water to obtain solution A; dissolve chitosan in acetic acid solution to obtain solution B; S2, mixing the extract, the emulsifier and water uniformly to obtain solution C; S3, slowly dropping solution C into solution A to obtain an emulsion, and then slowly dropping the emulsion into solution B. After the reaction is completed, filtering, washing, and drying are performed to obtain the extract microcapsules.

5. A method for preparing the tangerine peel composite protein powder according to claims 1-4, characterized in that: The following steps are involved: S1, weigh the raw materials according to the proportion; S2, premixing the isolated soy protein powder and the resistant dextrin by low-speed shearing; S3, adding tangerine peel extract microcapsules, daisy flower extract microcapsules and polydextrose to perform high-speed shear mixing; S4. Finally, the remaining raw materials are added for secondary high-speed shear mixing. After all the raw materials are evenly mixed, the tangerine peel composite protein powder is obtained.

6. The method for preparing a tangerine peel composite protein powder according to claim 5, wherein: The shear rate of the low-speed shear premixing is 100-500rpm, the mixing time is 5-15min, and the mixing temperature is 20-40°C; the shear rate of the first high-speed shear mixing is 500-2000rpm, the mixing time is 3-10min, and the mixing temperature is 25-45°C; the shear rate of the second high-speed shear mixing is 1000-3000rpm, the mixing time is 8-15min, and the mixing temperature is 30-50°C.

7. Use of the tangerine peel composite protein powder according to claim 1 in alleviating symptoms such as nausea and bloating during weight loss and maintaining muscle mass.

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