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New application of immature bitter orange or immature bitter orange extract

A citrus aurantium extract and its application technology, applied in the field of citrus aurantium or citrus aurantium extract, to achieve the effect of reducing colon frequency, significant curative effect, and improving intestinal sensitivity

Inactive Publication Date: 2012-04-18
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no report on the use of Citrus aurantium and its extracts for the treatment of irritable bowel syndrome

Method used

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  • New application of immature bitter orange or immature bitter orange extract
  • New application of immature bitter orange or immature bitter orange extract
  • New application of immature bitter orange or immature bitter orange extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 Preparation of Fructus Fructus Aurantii Extract of the present invention

[0026] (1) Take the coarse powder of Citrus aurantium, add water to decoct for 8 hours, add 12 times the amount of water, and use the water extract and filter residue for subsequent use;

[0027] (2) Take the filter residue obtained in step (1), add 10 times the amount of 70% V / V ethanol to reflux and extract twice, each time for 1 hour, after recovering the ethanol, an alcohol extract is obtained;

[0028] (3) Combine the water extract of step (1) and the alcohol extract of step (2), after concentration, add 95% V / V ethanol to the alcohol content of 60% V / V, after alcohol precipitation, filter and precipitate For future use, the supernatant is concentrated, dried, and mixed with the precipitate to obtain the Citrus aurantium extract.

[0029] Determination of total flavonoids content in Citrus citrus extract:

[0030] The content of total flavonoids in Citrus aurantium was determi...

Embodiment 2

[0034] Example 2 Determination of Nobiletin and Naringin in the Fructus Aurantii Fructus Extract of the present invention

[0035] Chromatographic conditions: octadecylsilane bonded silica gel as filler; mobile phase: acetonitrile-water (23:77); flow rate 1ml / min (detection wavelength: 283nm).

[0036] Preparation of the reference solution: dry the color-changing silica gel for 24 hours, and prepare naringin 12ug / ml and nobiletin 14ug / ml with water.

[0037] The preparation of need testing solution: get citrus citrus extract 0.3g (prepared in Example 1), accurately weighed, put in a round bottom flask, add methanol 30ml, shake up, put in a water bath for reflux extraction 1h, take out, and cool to room temperature , filter, wash the container, filter paper and medicinal residues with methanol in stages, combine the lotion with the filtrate, evaporate to dryness, dissolve the residue in methanol and dilute to 25ml for later use. (Extract 12mg / ml)

[0038] Injection volume: 20...

Embodiment 3

[0040] Example 3 Determination of Synephrine Content in Citrus Fructus Citrus Fructus Extract of the Present Invention

[0041] Chromatographic conditions: use octadecylsilane bonded silica gel as filler; use methanol-potassium dihydrogen phosphate aqueous solution (take 0.6 g of potassium dihydrogen phosphate, 1.0 g of sodium dodecylsulfonate, 1 ml of glacial acetic acid, dissolve in water and diluted to 1000ml) (50:50) as the mobile phase; the detection wavelength is 275nm.

[0042] Preparation of the reference solution: the color-changing silica gel was dried for 24 hours, and the synephrine reference substance was 30ug / ml of water.

[0043] The preparation of need testing solution: get about 1g (prepared in Example 1) of Fructus Fructus Citrus Citrifolia extract, accurately weighed, put in a stoppered Erlenmeyer flask, accurately add methanol 50ml, weigh, heat and reflux for 1.5 hours, let cool, Weigh the weight again, make up the lost weight with methanol, shake well, fi...

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Abstract

The invention provides application of immature bitter orange or immature bitter orange extract to preparation of medicaments for treating irritable bowel syndrome. The invention also provides a medicinal composition based on the immature bitter orange or immature bitter orange extract. By improving bowel sensitivity and reducing colon frequency, peak potential, peak-to-peak value and peak area, the immature bitter orange extract therefore has an obvious curative effect in a treatment period of the irritable bowel syndrome, the belly ache caused by the irritable bowel syndrome can be suppressed, the phenomenon of colon dysfunction is improved, and a new choice is provided for clinical medication.

Description

technical field [0001] The invention relates to a new application of citrus aurantium or citrus aurantium extract. Belongs to the field of medicine. Background technique [0002] Irritable bowel syndrome (Irritable Bowel Syndrome, IBS) was first reported by Powell in 1820. The syndrome without abnormal morphological and biochemical changes is one of the most common functional gastrointestinal diseases in clinical practice. At present, the diagnosis of IBS is mainly based on symptomology and based on the exclusion of organic diseases. In recent years, many studies have shown that gastrointestinal motility disorder and visceral abnormality are the main pathophysiological basis of the symptoms of this disease, and its pathogenesis is obviously affected by psychosocial factors. Research reports from various places show that IBS is a common and frequently-occurring disease worldwide, and its incidence rate is quite high. According to epidemiological surveys, the prevalence ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/752A61P1/00
Inventor 彭成李瑜朱力阳郭力
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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