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Novel function and application of seville orange flower extract

A technology of extracts and generations of flowers, applied in the field of radiation protection, can solve the problems of less research on anti-oxidation and UV protection functions, and achieve the effect of broadening the scope of functions and applications

Inactive Publication Date: 2015-03-25
SHENZHOUSPACEBIOTECHGRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the antioxidant and UV protection functions of flavonoids such as hesperidin and neohesperidin.

Method used

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  • Novel function and application of seville orange flower extract
  • Novel function and application of seville orange flower extract
  • Novel function and application of seville orange flower extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 anti-aging experiment

[0024] 1. Promote cell proliferation

[0025] ESF cells were adjusted to a cell concentration of 4 × 10 4 / ml, seeded in a 24-well plate, 0.5ml / well, that is, 2×10 4 a / well. The cells were cultured overnight and after the cells adhered to the wall, they were cultured with a medium containing 1% FBS for 24 hours. The cells were divided into two groups, namely the control group (the culture medium was not added with Daidaihua extract), and the experimental group (the culture medium was added with different concentrations of Daidai flower extract). The added concentration of Daidaihua extract in the experimental group were: 10 μg / ml, 50 μg / ml, 100 μg / ml. After 72 hours, remove the medium, wash once with DPBS, add 200ul of new culture solution containing 10% CCK-8, incubate at 37°C for about 1 hour, take the supernatant in a 96-well plate, and use a microplate reader to detect the supernatant at a wavelength of 450nm Liquid OD value...

Embodiment 2

[0033] Embodiment 2 anti-radiation experiment

[0034] Hacat cells and ESF cells in 4×10 4 cells / well seeded in 12-well plate, or 2x10 5 The cells / wells were seeded in 6-well plates, and were prepared for UVB irradiation treatment when the cells were cultured to more than 80% confluence.

[0035] 1. Content detection of inflammatory factors IL-1α, IL-6 and TNF-a

[0036] The cells were divided into 3 groups, namely the control group (no UVB treatment, medium without Daidaihua extract added), UVB group (UVB treatment, medium without Daidaihua extract added), UVB+Dadaihua group (UVB treatment , the culture medium was supplemented with different concentrations of Daidaihua extract). The concentrations of the extracts of Daidaihua in the UVB+Daidaihua group were: 10 μg / ml, 50 μg / ml, and 100 μg / ml.

[0037] After 24 hours of cell inoculation, the cells sucked out the medium, added PBS to wash the cells once, removed the PBS, and placed them in an ultraviolet irradiation box for...

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PUM

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Abstract

The invention relates to a novel function and application of seville orange flower extract. Particularly, hesperidin, neohesperidin and other flavonoid substances in the seville orange flower extract are capable of promoting cell proliferation and expression of mRNA of fibrocyte IV-type collagen, inhibiting activities of elastase and matrix metalloproteinase, reducing damage of ultraviolet ray to cells and reducing the level of reactive oxygen species and secretion of inflammatory factors, so that the seville orange flower extract has a function of resisting radiation and aging for skins. Therefore, the invention provides a new direction of seville orange flower extract application, namely application of the seville orange flower extract in skin protecting products.

Description

technical field [0001] The present invention relates to a new function of Daidaihua extract, in particular to the new function of Daidaihua extract for radiation protection and anti-aging and its application. Background technique [0002] It belongs to the variant of Rutaceae citrus plant lime from generation to generation, distributed in various parts of southern my country, and cultivated in Zhejiang, Jiangsu, Guangdong, Guizhou and other places. Daidaihua is sweet in taste, bitter in taste, flat in nature, can soothe the liver and regulate qi, harmonize the stomach and stop vomiting. The immature peel contains flavonoids such as neohesperidin, naringin, and hesperidin, and the main components of the volatile oil are limonene, linalool, dehydrolinalool, and eugenol. [0003] The current research on Daidaihua is mainly to use the volatile oil contained in it to produce spices. Daidaihua extract is used as spices in fields such as tobacco. At the same time, studies in rec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61Q19/08A61Q17/04A23L1/30A23L2/38A61P39/06A23L33/105
Inventor 党磊王小雪张美姿庞欣曹茜
Owner SHENZHOUSPACEBIOTECHGRP
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