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New application of ascorbic acid

A technology for ascorbic acid and new use, applied in the field of ascorbic acid, can solve the problems of immature technology, unclear mechanism of somatic cell reprogramming, low efficiency, etc., and achieve the effect of strong scientificity and innovation, and high development and application value.

Inactive Publication Date: 2010-06-23
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, all these techniques for inducing somatic cell reprogramming are immature and inefficient, and the mechanism of somatic cell reprogramming is still unclear

Method used

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  • New application of ascorbic acid
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  • New application of ascorbic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Using VC to improve the reprogramming efficiency of mouse somatic cells.

[0041] a, A total of 20,000 OG2 mouse embryonic fibroblasts (MEFs) were infected in one well of a 12-well plate with four-factor (SKOM) or three-factor (SKO) viruses mixed in equal volumes (1ml for each virus) , cultured in mES medium at 37°C 5% CO2. From D2 days after the end of two infections, add VC or other compounds (VC, 50ug / ml; Vb1, 9ug / ml; NAC, 1mM; GMEE, 1.5ug / ml; Selenite, 20nM; antioxidant mixture Antiox, namely VC +Vb1+GMEE+Selenite); For four factors, D9-10 days for digestion, for three factors, D15-16 days for digestion, the proportion of GFP positive cells was detected by flow cytometry.

[0042] Such as figure 1 As shown, the addition of VC or various antioxidants containing VC significantly increased the iPS efficiency of both SKOM and SKO compared with the control group.

[0043] b, As mentioned above, divide the three-factor SKO-iPS cells into the trophoblast cells...

Embodiment 2

[0054] Example 2 Using VC to promote the transformation of mouse iPS precursor cells into complete iPS cells.

[0055] Most of the four-factor SKOM-iPS clones induced by classical mES medium did not express GFP, which initially showed that these cells did not eventually reach the state of complete iPS cells. We picked out these clones and serially passaged them, such as Figure 4 As shown, after 5-9 generation VC treatment, about 3 / 4 clones showed obvious expression of GFP. The morphology of these clones was also remarkably close to that of mESC cells, whereas serial passage of these clones in classical mES medium resulted in loss of mESC morphology. Therefore, at least part of the promoting effect of VC on iPS is reflected in the transformation of most precursor iPS cells into complete iPS cells.

Embodiment 3

[0056] Example 3 Using VC to maintain stable passage of mouse iPS cells.

[0057] Similar to Example 2, we picked the GFP-positive iPS clones, and successively passaged them in the classic mES medium and the medium added with VC respectively. After about 5 passages, as Figure 5 As shown, in the classic mES medium, the fluorescence of iPS clones was significantly lost, and the clones also lost their mESC morphology; while the medium added with VC could relatively stably maintain the GFP fluorescence expression of iPS and the mESC morphology of the cells. This shows that VC can maintain the mESC state of mouse iPS cells.

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PUM

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Abstract

The invention relates to new application of ascorbic acid, that is, the ascorbic acid has the anti-ageing function by a cell reprogramming mechanism, so as to provide direct and powerful experimental basis and theory basis for the anti-ageing application of the ascorbic acid; therefore, the invention has strong scientificalness and innovativeness and has high development and application value.

Description

technical field [0001] The present invention relates to the use of ascorbic acid, in particular to the new use of ascorbic acid for anti-aging through cell reprogramming mechanism. Background technique [0002] Ascorbic acid, also known as vitamin C, is a water-soluble vitamin. Its main functions are: 1. Promote the biosynthesis of bone collagen, which is beneficial to the faster healing of tissue wounds; 2. Promote the metabolism of tyrosine and tryptophan in amino acids , prolong the life of the body; 3. Improve the utilization of iron, calcium and folic acid; 4. Improve the metabolism of fats and lipids, especially cholesterol, and prevent cardiovascular diseases; 5. Promote the growth of teeth and bones, prevent gum bleeding, and prevent joint pain , Lumbago and leg pain; 6. Enhance the body's anti-stress ability and immunity to the external environment; 7. Water-soluble and strong antioxidants, mainly acting in the aqueous solution in the body; 8. Strong connective tiss...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/375A61P39/06A23L1/29C12N5/0735A23L33/00
Inventor 裴端卿米盖尔.埃斯特班王涛秦宝明杨佳银
Owner GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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