Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing high-bioavailability derivatives of hesperidin

A technology of hesperidin and utilization rate, which is applied in the field of preparation of derivatives with high bioavailability, can solve problems such as low yield, low hydrolysis rate, and difficulty in separation and purification, and achieve improved physical and chemical properties and bioavailability, solvent The effect of low consumption and simple process

Inactive Publication Date: 2009-06-03
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with acid hydrolysis, the enzymatic hydrolysis temperature is lower, and the hydrolysis reaction is better controlled, but the yield of enzyme produced by fermentation is not high, the price is expensive, and the hydrolysis rate is low. If the enzyme system contains multiple enzymes, the obtained The reaction products are still complex, making separation and purification difficult

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Hesperidin was purchased from Zhejiang Quzhou Tiansheng Plant Extraction Co., Ltd., with a content of 96% (HPLC). Weigh 3g of hesperidin, dissolve in 15ml of 1M sodium hydroxide solution, add 20ml of isopropanol, preheat, fully dissolve, add 18ml of preheated 5M hydrochloric acid solution, add water to 50-60ml, heat to boil Reflux for 15 minutes to terminate the reaction, adjust the pH value to 4, let stand, and filter to obtain 2.2 g of the hydrolyzed product. Through HPLC analysis, it can be seen that the hydrolysis rate of hesperidin is 17.31%, and the content of hesperetin-7-glucoside and hesperetin They are 16.26% and 4.36% respectively. The obtained hydrolyzate was separated and purified to obtain 0.6 g of hesperetin-7-glucoside product with a purity of 64%, and 0.2 g of hesperetin product with a purity of 84%.

Embodiment 2

[0015] Example 2: Weigh 3g of hesperidin, dissolve it in 15ml of 1M sodium hydroxide solution, add 20ml of isopropanol, preheat, add 18ml of preheated 5M hydrochloric acid solution, heat to boil and reflux for 45min to obtain hydrolysis The product was 2.3g. According to HPLC analysis, the hydrolysis rate of hesperidin was 35.53%, and the contents of hesperetin-7-glucoside and hesperetin were 28.05% and 10.06%, respectively. The hydrolyzate was separated and purified to obtain 1 g of hesperetin-7-glucoside product with a purity of 70%, and 0.4 g of hesperetin product with a purity of 86%.

Embodiment 3

[0016] Example 3: Weigh 3g of hesperidin, dissolve it in 15ml of 1M sodium hydroxide solution, add 20ml of ethanol, preheat, add 18ml of preheated 5M hydrochloric acid solution, heat to boil and reflux for 45min, and obtain 2.5g of hydrolyzate , through HPLC analysis, it can be seen that the hydrolysis rate of hesperidin is 9.18%, and the contents of hesperetin-7-glucoside and hesperetin are respectively 8.68% and 4.50%. The hydrolyzate was separated and purified to obtain 0.22 g of hesperetin-7-glucoside product with a purity of 63%, and 0.1 g of hesperetin product with a purity of 82%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing high-bioavailability derivatives of hesperidin, which is characterized in that the method obtains hesperetin-7-glucoside and hesperetin with higher purity by appropriate hydrolysis of the hesperidin and a certain separation and purification method. The preparation method is as follows: the hesperidin extracted from citrus reticulata is taken as raw material and dissolved in a certain amount of alkaline solution, a certain amount of organic solvent is added, the mixture is pre-heated after the full dissolution, a certain amount of hydrochloric acid which is also pre-heated is finally added, and hydrolysis mixed products are obtained by refluxing in a boiling water bath for a certain time, cooling, regulating pH, standing and filtering. The hesperetin-7-glucoside and the hesperetin are obtained by separating and purifying the mixture. The hesperidin which is not decomposed after separating and purifying the products can be used for recycling in the next acid hydrolysis. The method can obtain the hesperetin-7-glucoside and the hesperetin which are the high-bioavailability derivatives of the hesperidin by the simple acid hydrolysis method, thereby having the advantages of low cost and simple process; furthermore, the method can realize the recycled production and is easy to realize the industrial production.

Description

1. Technical field [0001] The invention relates to a preparation method of a derivative with high bioavailability of hesperidin, which belongs to the field of secondary deep processing of citrus functional components. 2. Background technology [0002] Hesperidin is one of the most abundant flavonoids in citrus, but hesperidin exists in the form of glycoside with low activity in citrus fruit, and the glycosidic bond of the extracted hesperidin has not been broken. The existence of such glycosidic bonds, especially the presence of rhamnosidic bonds, hinders its digestion and absorption by organisms and reduces its bioavailability. Moreover, the solubility of hesperidin is very poor, and its water solubility and fat solubility are quite low, making it difficult to be utilized by biological enzymes and difficult to penetrate biological cell membranes, thus limiting its application in food and other fields. [0003] The extraction of hesperidin from citrus has been industrialize...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07D311/32C07D311/40
Inventor 郑美瑜陆胜民陈剑兵杨颖夏其乐邢建荣
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com