Beta-glucuroide isozyme BglA protein and application thereof

Abstract

The invention discloses amino acid sequences shown as SEQ ID NO:1 and SEQ ID NO:2 of a gene for coding beta-glucuroide isozyme BglA. The BglA protein with high beta-glucuroide activity is cloned and expressed by using an ATCC (American Type Culture Collection) standard fungus strain S.singularis and adopting a modern molecular biology, aurantiamarin is biologically converted by using the BglA protein, and the glycoside is converted into aglycon, application individual difference in people of glycoside traditional Chinese medicine is eliminated, the bioavailability of the glycoside traditional Chinese medicine is improved, and the pharmacological response of active components of the glycoside traditional Chinese medicine is enhanced. In the invention, pharmacokinetics parallel comparative experiments of two dose modes of an oral dose and a vein dose of the aurantiamarin and hesperetin are simultaneously carried out in a laboratory room for the first time, which finds that the hesperetin is easy to absorb and is more slowly eliminated in comparison with the aurantiamarin, thereby complementing the reason of the bioavailability of the aurantiamarin lower than that of the aglycon.

Description

technical field [0001] The invention belongs to the technical field of microbial transformation engineering, and relates to a protein of β-glucosidase isozyme BglA and its application. More specifically, it is a method for improving the bioavailability of hesperidin in tangerine peel by using β-glucosidase isoenzyme BglA protein to carry out biotransformation of tangerine peel. Background technique [0002] The essence of biotransformation is that one or several special extracellular or intracellular enzymes produced by microorganisms are used as biocatalysts to perform one or several chemical reactions on exogenous substrates. Biotransformation can not only increase the yield of the target product, overcome the shortcomings of chemical synthesis, but also because most of them work at room temperature and in a neutral environment, it reduces product decomposition, isomerization, racemization and rearrangement reactions, and the reaction has position selectivity And stereose...

AI Technical Summary

Problems solved by technology

However, strong chemical reactions will change the structure and configuration of glycoside compounds, resulting in unstable products or loss of drug efficacy; enzymatic hydrolysis, such as the Swiss Nestlé Research Center using enzyme modification methods, using hesperidinase to hydrolyze hesperidin to obtain hesperetin -7-glycoside

The enzymatic method of transforming glycosides traditional Chinese medicine uses glycoside extracts as the substrate and glycoside hydrolase as the catalyst. The process is complicated and not conducive to industrial production.

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