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Method for simultaneously measuring 11 flavonoid constituents in vine tea through HPLC

A technology of flavonoids and rattan tea, which is applied in the field of medicinal plant component analysis, can solve problems such as differences in the content of flavonoids, and achieve the effects of accurate and reliable results, good symmetry, and sharp peak shapes.

Inactive Publication Date: 2016-06-29
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

However, due to differences in varieties, ecological environments, and cultivation and management conditions, there are significant differences in the content of flavonoids in rattan tea.

Method used

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  • Method for simultaneously measuring 11 flavonoid constituents in vine tea through HPLC
  • Method for simultaneously measuring 11 flavonoid constituents in vine tea through HPLC
  • Method for simultaneously measuring 11 flavonoid constituents in vine tea through HPLC

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Embodiment Construction

[0021] In order to make the content of the present invention easier to understand, the technical solutions of the present invention will be further described below in conjunction with specific embodiments, but the present invention is not limited thereto.

[0022] 1 Experimental part

[0023] 1.1 Instruments and reagents

[0024] ThermoFisher LCQFleet high performance liquid chromatography-mass spectrometry, equipped with quaternary gradient pump, two-tube array detector, automatic sample injector; Sartorius BT125D analytical balance (Sartorius Scientific Instruments (Beijing) Co., Ltd.); high-speed centrifuge (Sigma3K-30); ultraviolet and visible spectrophotometer (ThermoScientificEvolution201); freeze dryer (TelstarLyoQuest-55).

[0025] The standard products of dihydromyricetin, dihydroquercetin, orangenin, naringenin, hesperetin, myricetin, myricetin, kaempferol, quercetin, rutin and apigenin were purchased from Shanghai Tongtian Biology Co., Ltd. Technology Co., Ltd. (p...

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Abstract

The invention discloses a method for simultaneously measuring 11 flavonoid constituents in vine tea through HPLC and belongs to the technical field of medicinal plant constituent analysis.The method includes the steps that firstly, a mixed standard substance solution and a vine tea test sample solution are prepared respectively, then the mixed standard substance solution and the vine tea test sample solution are detected respectively through high performance liquid chromatography, and the contents of dihydromyricetin, dihydroquercetin, aromadendrin, naringenin, hesperetin, myricetin, myricetrin, kaempferol, quercetin, rutin and apigenin in a test sample are obtained through calculation.The measuring method is good in stability and reproducibility, easy to operate, accurate and reliable in result and capable of being applied to simultaneous measurement of multiple flavonoid constituents in the vine tea.

Description

technical field [0001] The invention belongs to the technical field of component analysis of medicinal plants, and in particular relates to a method for simultaneously determining 11 flavonoid components in rattan tea by using HPLC. Background technique [0002] Vine tea is a kind of wild vine in the genus Snake Grape of the family Vitaceae, and its scientific name is Snake Grape ( Ampelopsis grossedentata W.T.Wan), is a unique medicinal and edible plant in my country, mainly distributed in mountainous areas with an altitude of 200-1300m in the south of the Yangtze River Basin and other provinces in my country. Vine tea is sweet, light, and cool in nature. It has the functions of clearing heat, detoxifying, and dispelling rheumatism. It is commonly used by folks to treat colds, fever, sore throat, jaundice hepatitis, and blisters. It has a history of thousands of years. Modern pharmacological studies have shown that vine tea has anti-oxidation, antibacterial and anti-inflam...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N21/33
CPCG01N21/33G01N30/02G01N2030/027
Inventor 许明郑金贵刘江洪曹晓华杨志坚
Owner FUJIAN AGRI & FORESTRY UNIV
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