323 results about "Dihydro-myricetin" patented technology

The invention relates to a method for extracting dihydromyricetin, ampelopsis grossedentata amylase and ampelopsis grossedentata polyphenol from ampelopsis grossedentata. The method comprises the following processing steps of: (a) crushing raw materials: drying and crushing fresh ampelopsis grossedentata stem leaves; (b) leaching: extracting with hot water and centrifugally collecting supernatant fluid; (c) concentrating: decompressing and concentrating the supernatant fluid I, cooling, standing still and centrifugally collecting precipitate and supernatant fluid II; (d) extracting dihydromyricetin: recrystallizing and drying the precipitate in vacuum to obtain dihydromyricetin; (e) alcoholizing: decompressing and concentrating the supernatant fluid II, depositing in alcohol and centrifugally collecting a precipitate and supernatant fluid III; (f) extracting crude polysaccharides: purifying the precipitate, drying in vacuum or spraying and drying to obtain ampelopsis grossedentata amylase; and (g) extracting ampelopsis grossedentata polyphenol: decompressing and concentrating the supernatant fluid III, extracting with ethyl acetate, concentrating and drying to obtain ampelopsis grossedentata polyphenol or absorbing with AB-8 resin, eluting with distilled water, eluting with ethanol and collecting eluate, concentrating and drying in vacuum to obtain ampelopsis grossedentata polyphenol. The method has the advantages of low cost, higher product yield and environmental protection, is suitable for industrial continuous production and can comprehensively develop and utilize ampelopsis grossedentata resources and improve the economic benefits.

The invention discloses application of dihydromyricetin in preparing a medicine for preventing and treating untoward reactions in radiotherapy and chemotherapy of tumor. In particular, the application is to prepare medicines for preventing and treating untoward reactions in radiotherapy and chemotherapy of tumor, preventing and treating arrest of bone marrow and baldness, resisting mutation, preventing and treating the generation of secondary tumors, preventing and treating tumor transfer, and preventing and treating breast cancer, cervical carcinoma, intestinal cancer and the like. The invention creatively finds that the dihydromyricetin has the effects of removing free radicals, inhibiting reaction chains of the free radicals, resisting the mutation, inhibiting the expression of the tumor gene, causing the death of tumor cells, preventing and treating the tumor generation, preventing and treating the secondary tumors and the transfer of the secondary tumors and preventing and treating infection. Therefore, as the medicine for preventing and treating untoward reactions in radiotherapy and chemotherapy of tumor, the dihydromyricetin can inhibit and alleviate chemical damage, prevent and treat damage of a mutagen and further prevent gene mutation or the secondary tumors; and by combination with the radiotherapy and chemotherapy, the effects of preventing untoward reactions in radiotherapy and chemotherapy of tumor and preventing generation of tumors are achieved.

The invention discloses a method for preparing total flavonoids of hovenia acerba. The method adopts one optional method of the solvent extraction method, the solvent extraction method, the macro-porous resin adsorption method, the liquid-liquid countercurrent distribution chromatography, the supercritical fluid extraction method or the column chromatography, or the free combination of the methods. The total flavonoids of hovenia acerba is prepared from any one part of hovenia acerba, such as fruit stems, fruits, seeds, seed-cases, roots, stems, leaves, flowers, branches, skins, skin and fruit residues, etc., and the combination thereof. The prepared total flavonoids is a combination of various flavonoid active components, wherein the total flavonoids comprises the main active components as follows: quercetin, kaempferol, (+)-dihydromyricetin, myricetin, north hovenia acerba alcohol, (+)-pyrocatechin, hovenia acerba I, hovenia acerba II, hovenia acerba III and the derivates thereof; the total percentage composition of various flavonoid components by the weight is 5% to 100%; and the preparation method is applicable to pharmaceutical industry, food industry, cosmetic industry and health-care product industry.

The invention discloses a dihydromyricetin cyclodextrin inclusion compound, which comprises cyclodextrin and dihydromyricetin, wherein the dihydromyricetin is embedded into a cyclodextrin cavity; and the inclusion compound employing the cyclodextrin as a host molecule and dihydromyricetin as a guest molecule is formed. The dihydromyricetin cyclodextrin inclusion compound has the beneficial effects that 1 the dihydromyricetin cyclodextrin inclusion compound is embedded by the cyclodextrin, and the solubility, the stability and the bioavailability of the dihydromyricetin are improved; 2 the preparation method of the dihydromyricetin cyclodextrin inclusion compound disclosed by the invention is simple in process and suitable for industrialized production; 3 a spray drying process is adopted, the process from spray drying to dust collection is finished within 20 seconds, the problem of product oxidation in the drying process is avoided, the method is simple in process, simple and convenient to operate; the production conditions are easy to control, and the inclusion rate of the dihydromyricetin is high; and 4 the dihydromyricetin used by the dihydromyricetin cyclodextrin inclusion compound is extracted from vitaceae ampelopsis, and the dihydromyricetin in the vitaceae ampelopsis is high in content, and is a natural substance and free of toxicity.

The present invention discloses the process of extract dihydromyricitrin from vine tea. The process includes soaking vine tea leaf in solvent, filtering, microwave irradiating wet vine tea leaf, merging filtrate, extracting in heating water bath, filtering the hot extracting liquid, dissolving the filtrate, and recrystallization in cold water for purifying. The process has high extraction rate, simple operation and less side product.

The invention discloses a method for extracting dihydromyricetin from stem leaves of Ampelopsis sinica(Miq.)W.T.Wang of Ampelopsis Michx and stem leaves of Ampelopsis grossedetata(Hand- Mazz)W.T.wang, a medicine preparation and new medical application. The dihydromyricetin can be applied to prevention and treatment of senile dementia. A prepared medicine can either contain the dihydromyricetin only, or be a composition containing the dihydromyricetin, and can be pharmaceutical preparations, such as tablets, capsules, granules, and dripping pills or injections.

A method for preparing the dish 'Fish pickled with distiller's grains' includes such steps as pre-treating raw materials, salting, drying in the sun, pickling with distiller's grains while adding bihydromyricetin and flavone-type antioxidant for fermenting, and sealing.

The invention provides a method for producing diverse extracts of berry tea, comprising the following steps: drying Ampelopsis grossedentata tender stems rattans and leaves, crushing, carrying out microwave extraction, macroporous resin separation, concentrating column-over effluent liquid at reduced pressure, carrying out ethanol precipitation on concentrated liquid, and drying ethanol precipitation substance, so as to obtain a berry tea polysaccharide extract; eluting desorption liquid, collecting desorption eluent, concentrating, and carrying out spray drying on the concentrated liquid, soas to obtain a berry total flavone extract; and ultrafiltrating the desorption eluent, passing though a reverse osmosis membrane, concentrating retentate, adding 3-5% of active carbon, filter pressing, cooling and crystallizing filtrate, centrifugally dewatering and vacuum drying so as to obtain crude dihydromyricetin. The method provided by the invention is in favor of comprehensively using Ampelopsis grossedentata plant resources; and the microwave extraction, macroporous resin elution and membrane separation methods based on water as a medium are used, thereby being beneficial to reductions of use amount of organic solvents, energy consumption, production cost and environment pollutions.

The invention provides a vine tea extract which is prepared by the following steps: adding a flavones protective agent into an aqueous solution during water extraction of the vine tea; heating and filtering; adding a co-precipitator into the filtrate for flocculation and sedimentation; and after filtering the precipitate, freezing the filtrate to crystallize and separate out the vine tea extract, wherein the vine tea extract comprises 80-90% of general flavones and 70-80% of dihydromyricetin based on general flavones. The invention further provides a composition which comprises the vine tea extract and a ginkgol biloba extract as well as an application of the composition in preparing foods, health foods and drugs for preventing and treating geriatric cognitive disorders. The invention further provides an application of the vine tea extract in preparing drugs for inhibiting prostatic hyperplasia and treating benign prostatic hyperplasia, as well as foods, health foods and drugs for reducing blood sugar, blood fat and blood pressure. Compared with the extract prepared by the existing method, the extract provided by the invention is improved in purity and white in luster. Clinical applications show that the vine tea extract has a certain function of improving getting up in the night to urinate. The vine tea extract combined with the ginkgo biloba extract can be used for preventing and improving geriatric cognitive disorders.

An application of dihydromyricetin as the additive of forage for improving the quality of meat is disclosed. Said dihydromyricetin is prepared from the stem and leaf of amur ampelopsis through extracting in alcohol, concentrating, drying to obtain coarse flavone, reflux extracting in acetone, vacuum concentrating, adding water, educing out crystals, and recrystallizing.

The invention discloses a method for extrating dihydromyricetin from Hovenia Dulcis Thunb. The method comprises following steps: pretreating medicinal materials and getting extracting liquid of dihydromyricetin, purifying with resin, recrystallizing and getting high- purity dihydromyricetin (the purity is over 99%). The invention is characterized by easy operation, less toxicity, low cost, convenient control and feasibility for large- scale production.

The invention discloses a method for extracting dihydromyricetin and Ampelopsis grossedentata polysaccharide from Ampelopsis grossedentata, which comprises the following steps: (1) drying, and pulverizing; (2) soaking in a solvent; (3) carrying out microwave extraction; (4) heating the extracting solution, filtering with a ceramic membrane to remove the suspension, and passing the obtained filtrate through an ultrafiltration membrane while the filtrate is hot; (5) concentrating the filtered solution, crystallizing, and drying; and (6) preparing the part which does not pass through the ultrafiltration membrane into Ampelopsis grossedentata polysaccharide. By using the method combining microwave reflux extraction and membrane separation extraction, the technique is simple and convenient to operate, enhances the extraction rate and purity of dihydromyricetin extracted from Ampelopsis grossedentata (the extraction rate of dihydromyricetin is up to 68.5%, and the purity is up to 83%), and can also be used for preparing the Ampelopsis grossedentata polysaccharide. The method has the advantages of low consumption of organic solvent required by extraction, and high operational safety, does not need high-temperature furnace heating, lowers the energy consumption, and saves the production cost.

The invention discloses applications of dihydromyricetin in preparation of anti-influenza virus drugs, wherein influenza virus is influenza A virus, preferably H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 or H10N8. According to the present invention, the anti-influenza A virus action mechanism of the dihydromyricetin is that the activity of the ribonucleoprotein complex vRNP is inhibited by binding the dihydromyricetin to the PB2cap protein so as to prepare the drugs for prevention and treatment of influenza, wherein the binding loci of the dihydromyricetin and the PB2cap protein are site-432 histidine (His432), site-357 histidine (His357), site-404 phenylalanine (Phe404), site-323 Phenylalanine (Phe323), site-361 glutamic acid (Glu361), site-376 lysine (Lys376), site-363 phenylalanine (Phe363), and site-429 asparagine (Asn429).

The present invention discloses an application of dihydromyricetin in preparation of medicine for preventing or curing angiocardiopathy. The animal tests show that the dihydromyricetin has the effect for resisting internal phlebothrombosis, can be used for effectively preventing angiocardiopathy due to thrombosis, for example cardiac disease and apoplexy. Said invention can be made into medicine or health-care product.

The invention discloses an application of dihydromyricetin and the esterified product serving as antioxidant in high polymer, and the dosage is only 0.01-10.00 percent of the quantity of high polymer. The extract and the esterified product have sound heat stability, little toxicity, and have remarkable anti-oxidation action to various high-polymer materials, and can be extensively used as the antioxidant of various natural and synthesized high polymers.

The invention provides medicinal exocarpium tea and a process for preparing the same. By the aid of the medicinal exocarpium tea and the process, problems that single exocarpium or berry tea is not suitable to be drunk for a long term and the like can be solved, and rhoifolin in exocarpium and dihydromyricetin in berry tea can be sufficiently utilized. The process includes steps of flowery-flavor concentrated liquid extraction, exocarpium extract extraction, aroma enhancement, mixing, packaging and the like to obtain a tea product. The medicinal exocarpium tea and the process have the advantages that functional ingredients of exocarpium tea and the berry tea can be sufficiently utilized, the medicinal exocarpium tea is suitable to be drunk by people for a long term and has good taste, and tea soup is good in color and luster; owing to heating aroma enhancement modes, the medicinal exocarpium tea is also suitable to be drunk by people who suffer from pollen allergy, accordingly, the service range of scented tea can be expanded, and the medicinal exocarpium tea and the process are favorable for integration and healthy development of various fresh flower and tea industries; combined merits of the traditional tea and instant tea can be sufficiently utilized, the dissolution speed of first-brewing can be particularly increased, and the quality of the product can be improved.

Use of dihydromyricetin (DMY) as an NF-κB inhibitor or an IKK-β inhibitor for the treatment of arthritis, cancer, autoimmune conditions and other disease is provided. A pharmaceutical composition comprising DMY is also provided.

Extraction dihydromyricin from microwave and its composition are disclosed. The process is carried out by adding vitaceae plant materials in microwave extraction pot, adding extraction solvent by proportion of 5-10, extracting for 1-15mins at rated horsepower 600w-2000kw and temperature 50-100íÒC, filtering, cooling filtrate, depositing dihydromyricin and composition crystal on bottom of filtrate container, filtering, drying and obtaining dihydromyricin and composition crystal.

The invention discloses a process method for extracting high-content dihydromyricetin from ampelopsis grossedentata. The process method utilizing the ampelopsis grossedentata as a raw material includes steps of dried ampelopsis grossedentata extraction, concentration, cooling and crystallizing, solvent dissolving and impurity removal, decoloration, secondary concentration, crystallizing, recrystallizing, vacuum drying and the like and is convenient to operate and high in extraction rate.

The invention discloses a method for rapidly extracting high-purity dihydromyricetin in ampelopsis grossedentata leaves. The method comprises the following steps of (1) smashing dried leaves into 10-20 mesh; (2) immersion cleaning: putting into water and steeping for 6 hours at normal temperature, wherein the massic volume ratio of the material and liquid is 1:100(g/mL); (3) extracting: adding water again, extracting for 60 minutes at 95 DEG C, and separating the heat extracting solution, wherein the massic volume ratio of the material and the liquid is 1:25(g/mL); (4) crystallizing: cooling the extracting solution through refrigeration or at room temperature, promoting crystallization at 4-10 DEG C, filtering, thus obtaining crude product; and (5) recrystallizing: adding boiling water till the crude product is dissolved completely, recrystallizing, filtering mother liquid, thus obtaining the product. In step (1), if the raw material is fresh leaves, the fresh leaves need to be cut into pieces of 1-2mm, in step(2), the massic volume ratio of material and liquid for immersion cleaning is 1:80(g/mL). According to the method, the requirement on rapid preparation of high-purity dihydromyricetin product can be met through steps of immersion cleaning for once, extracting for once, and recrystallizing for once.

The invention discloses a plant antioxidant functional feed additive, which is prepared from the following raw materials in parts by weight: 0 to 10 parts of 2,6-di-tert-butyl-p-cresol, 1 to 10 parts of ethoxyquin, 1 to 5 parts of methionine hydroxy analog, 1 to 5 parts of talc powder, 1 to 10 parts of citric acid monohydrate, 1 to 80 parts of ampelopsis, and 5 to 20 parts of medical stones. The additive has the advantages that: the traditional natural plant ampelopsis is applied to livestock production, the ampelopsis containing natural plant anti-oxidation mould inhibitor dihydromyricetin has good effects of removing free radicals and resisting lipid peroxidation, and the problem of oxidizing grease in the feed rich in Cu<2+>, Fe<2+> and other metal ions can be solved; and therefore, the additive is a high-efficiency green functional anti-mould anti-oxidation additive product. The additive can be used as an excellent substitute product for chemically synthesized anti-mould anti-oxidation products, so that a new opportunity is brought to the development of feed industry, and establishment of a healthy culture production mode is facilitated.

The invention provides a chiral chromatographic separation and analysis method of dihydromyricetin enantiomer. The method comprises the following steps: dissolving a dihydromyricetin sample into the mobile phase, taking polysaccharide derivatives as the chiral stationary phase, and carrying out liquid chromatogram (LC) separation and analysis; wherein the mobile phase is a mixed solution of n-hexane and lower alcohols, and the polysaccharide derivative chiral stationary phase is one of stationary phases, which are individually covered by tri(3,5-dimethylphenylcarbamyl) straight-chain starch, tri((S)-(alpha)- phenylethylcarbamyl) straight-chain starch, tri(3,5- dimethylphenylcarbamyl)cellulose and tri(4- methylphenylcarbamyl)cellulose. According to the provided chiral separation and analysis method, the maximal separation degree of dihydromyricetin enantiomer is 1.95, and the maximal selection factor is 1.36.

The invention discloses a method for purifying dihydromyricetin, which comprises the following steps of: (1) dissolving coarse dihydromyricetin in a certain amount of solvent, and blending into a paste; (2) uniformly mixing the paste and a certain amount of adsorbent, and drying at the temperature of between 45 and 75 DEG C for 0.25 to 1 hour; (3) placing the mixture in a certain amount of water, filtering while hot, and standing the filter liquor over night; (4) filtering and drying to obtain dihydromyricetin crystal; and (5) repeating the operation once according to the steps (1) to (4) on raw materials with the coarse product content of less than 50 percent, wherein the solid liquid mass ratio, the adsorbent amount and the hot water amount for the second time are in the standard ratio based on the mass of the dihydromyricetin crystal, the rest indexes are invariable. The method for purifying the dihydromyricetin has the advantages of greatly reduction of the solvent consumption, no solvent loss, no operation such as heating the dissolved coarse products, distilling and recovering the solvent and the like, simple equipment, low input, energy conservation and environmental protection, fewer steps, high single processing capacity, low cost, and easy industrialized production in rural areas and farms.

The invention relates to modified hexagonal boron nitride, a prepreg, an epoxy resin thermal conductive composite material, a copper clad laminate as well as a preparation method and application of the prepreg. The modified hexagonal boron nitride is prepared by the following method: S1, carrying out ultrasonic dispersion on hexagonal boron nitride and dispersing into a dihydromyricetin solution,stirring, filtering and drying to obtain dihydromyricetin modified hexagonal boron nitride BN@DMY; and S2, carrying out heat treatment on the BN@DMY at 200 to 250 DEG C for 4 to 6 hours to obtain themodified hexagonal boron nitride. The modified hexagonal boron nitride disclosed by the invention is prepared by utilizing dihydromyricetin to modify and carrying out heat treatment; the dispersity ofboron nitride in epoxy resin can be greatly improved; when the modified hexagonal boron nitride served as thermal conductive filler is added into the prepreg, the epoxy resin thermal conductive composite material and the copper clad laminate, a small amount of the added filler can effectively improve thermal conductivity of the prepreg, the composite material and the copper clad laminate; and inaddition, the mechanical properties of the composite material and the copper clad laminate are high.

The invention discloses a method for simultaneously measuring 11 flavonoid constituents in vine tea through HPLC and belongs to the technical field of medicinal plant constituent analysis.The method includes the steps that firstly, a mixed standard substance solution and a vine tea test sample solution are prepared respectively, then the mixed standard substance solution and the vine tea test sample solution are detected respectively through high performance liquid chromatography, and the contents of dihydromyricetin, dihydroquercetin, aromadendrin, naringenin, hesperetin, myricetin, myricetrin, kaempferol, quercetin, rutin and apigenin in a test sample are obtained through calculation.The measuring method is good in stability and reproducibility, easy to operate, accurate and reliable in result and capable of being applied to simultaneous measurement of multiple flavonoid constituents in the vine tea.

The invention belongs to the field of chemical/natural products and discloses a method for controlling the racemization rate of dihydromyricetin. The method is realized by controlling the concentration of metal ions, especially of transition metal ions. By reducing the concentration of the metal ions, the racemization of the dihydromyricetin can be reduced; by increasing the concentration of the metal ions, the racemization of the dihydromyricetin can be promoted. By further controlling the temperature and the pH value, the racemization can be controller better so that optically-pure dihydromyricetin can be obtained during extraction and separation. The method is simple and convenient to operate, safe, efficient, economical and capable of being used for large-scale preparation of optically-pure dextro-dihydromyricetin.

The invention discloses a method for increasing the yield of dihydromyricetin in ampelopsis grossedentata. The method includes roasting ampelopsis grossedentata medicinal materials under the condition of temperatures of 80-120 DEG C for 1-2h; smashing the ampelopsis grossedentata medicinal materials to obtain ampelopsis grossedentata powder; uniformly mixing the ampelopsis grossedentata powder and ethyl alcohol with each other to obtain mixtures; ultrasonically extracting the mixtures and filtering the mixtures to obtain dihydromyricetin extract liquid; concentrating the dihydromyricetin extract liquid under reduced-pressure conditions, chilling and crystallizing the dihydromyricetin extract liquid in low-temperature environments, and filtering and drying the dihydromyricetin extract liquid to obtain filter cake which is a dihydromyricetin extract. The method has the advantages that the yield of the dihydromyricetin in samples obtained by means of high-temperature drying treatment is obviously higher than the yields of dihydromyricetin in samples dried in shade and normal dried samples, metabolism loss of the dihydromyricetin can be prevented, the extraction cost can be greatly lowered, the method is easy and convenient to implement and high in feasibility and is a method for efficiently and economically extracting the dihydromyricetin, the dihydromyricetin production efficiency can be obviously improved, the production cost can be lowered, and reasonable utilization of traditional Chinese medicine resources can be promoted.