Method for extracting dihydromyricetin and Ampelopsis grossedentata polysaccharide from Ampelopsis grossedentata

A technology of dihydromyricetin and vine tea, applied in the direction of organic chemistry and the like, can solve the problems of low extraction efficiency, low extraction purity, complicated extraction process, etc., and achieves improved extraction rate and purity, good operation safety, and simple process. Effect

Active Publication Date: 2012-11-28
贵州古耕农牧业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing methods for extracting dihydromyricetin from rattan tea generally have the

Method used

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  • Method for extracting dihydromyricetin and Ampelopsis grossedentata polysaccharide from Ampelopsis grossedentata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting dihydromyricetin and vine tea polysaccharide from vine tea, it comprises the following steps:

[0023] (1) Dry the fresh vine tea stems and leaves, and then crush them into 100-mesh vine tea particles;

[0024] (2) Soak the above vine tea granules in a solvent, and the ratio of solid to liquid between vine tea granules and solvent is 1:8;

[0025] (3) Add the mixed solvent to the microwave countercurrent extraction equipment for microwave extraction, repeat the operation 3 times, each extraction time is 10 minutes, and obtain the extract;

[0026] (4) Heat the extract to 40°C, first filter the suspension with a ceramic membrane, and then pass the obtained filtrate through the ultrafiltration membrane while it is hot, and the molecular weight cut-off of the ultrafiltration membrane is 8000 Daltons;

[0027] (5) The filtrate is concentrated, crystallized and dried;

[0028] (6) The part that failed to filter through the ultrafiltration membrane w...

Embodiment 2

[0033] A method for extracting dihydromyricetin and vine tea polysaccharide from vine tea, it comprises the following steps:

[0034] (1) Dry the fresh vine tea stems and leaves, and then crush them into 90-mesh vine tea particles;

[0035] (2) Soak the above-mentioned rattan tea granules in a solvent, the ratio of solid to liquid of rattan tea granules and solvent is 1:20;

[0036] (3) Add the mixed solvent to the microwave countercurrent extraction equipment for microwave extraction, repeat the operation twice, each extraction time is 15 minutes, and obtain the extract;

[0037] (4) Heat the extract to 50°C, first filter the suspension with a ceramic membrane, and then pass the obtained filtrate through the ultrafiltration membrane while it is hot, and the molecular weight cut-off of the ultrafiltration membrane is 50,000 Daltons;

[0038] (5) The filtrate is concentrated, crystallized and dried;

[0039] (6) The part that failed to filter through the ultrafiltration membr...

Embodiment 3

[0044] A method for extracting dihydromyricetin and vine tea polysaccharide from vine tea, it comprises the following steps:

[0045] (1) Dry the fresh vine tea stems and leaves, and then crush them into 95-mesh vine tea particles;

[0046] (2) Soak the above-mentioned rattan tea particles in a solvent, and the ratio of solid to liquid between rattan tea particles and solvent is 1:10;

[0047] (3) Add the mixed solvent to the microwave countercurrent extraction equipment for microwave extraction, repeat the operation 5 times, each extraction time is 8 minutes, and obtain the extract;

[0048] (4) Heat the extract to 45°C, first filter the suspension with a ceramic membrane, and then pass the obtained filtrate through the ultrafiltration membrane while it is hot, and the molecular weight cut-off of the ultrafiltration membrane is 20,000 Daltons;

[0049] (5) The filtrate is concentrated, crystallized and dried;

[0050] (6) The part that failed to filter through the ultrafilt...

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Abstract

The invention discloses a method for extracting dihydromyricetin and Ampelopsis grossedentata polysaccharide from Ampelopsis grossedentata, which comprises the following steps: (1) drying, and pulverizing; (2) soaking in a solvent; (3) carrying out microwave extraction; (4) heating the extracting solution, filtering with a ceramic membrane to remove the suspension, and passing the obtained filtrate through an ultrafiltration membrane while the filtrate is hot; (5) concentrating the filtered solution, crystallizing, and drying; and (6) preparing the part which does not pass through the ultrafiltration membrane into Ampelopsis grossedentata polysaccharide. By using the method combining microwave reflux extraction and membrane separation extraction, the technique is simple and convenient to operate, enhances the extraction rate and purity of dihydromyricetin extracted from Ampelopsis grossedentata (the extraction rate of dihydromyricetin is up to 68.5%, and the purity is up to 83%), and can also be used for preparing the Ampelopsis grossedentata polysaccharide. The method has the advantages of low consumption of organic solvent required by extraction, and high operational safety, does not need high-temperature furnace heating, lowers the energy consumption, and saves the production cost.

Description

technical field [0001] The invention relates to a method for extracting dihydromyricetin and vine tea polysaccharide from vine tea, and relates to the technical field of traditional Chinese medicine modernization. Background technique [0002] Vine tea, commonly known as Maoyan berry tea, Duanwu tea, and vine tea (also known as mountain sweet tea, dragon beard tea), is the tender product of Ampelopsis grossedentata (Hand-Mazz) W.T.wang. stems and leaves. Slightly bitter and sweet, it produces body fluid and quenches thirst; its taste is sweet and cool, and has the functions of clearing away heat and detoxification, antibacterial and anti-inflammatory, expelling wind and dampness, strengthening bones and muscles, lowering blood fat, and protecting liver and liver. [0003] Vine tea contains 19 kinds of nutrients and trace elements necessary for the human body. Its calcium, iron, and magnesium content are high, and it has a good preventive effect on cardiovascular diseases s...

Claims

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Application Information

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IPC IPC(8): C07D311/32C08B37/00
Inventor 罗文敏贾强孙超张珍明龙汉武向立容戚燕强
Owner 贵州古耕农牧业发展有限公司
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