Method for extracting dihydromyricetin, ampelopsis grossedentata amylase and ampelopsis grossedentata polyphenol from ampelopsis grossedentata

A technology of dihydromyricetin and rattan tea, applied in the direction of organic chemistry and the like, can solve the problems affecting the economic benefits of rattan tea resources, low resource utilization rate, etc., and achieve comprehensive development and utilization and economic benefits, low cost, and extraction yield. high effect

Inactive Publication Date: 2011-02-16
HUBEI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Using vine tea as raw material to extract any one of dihydromyricetin, vine tea polysaccharides, and vine tea polyphenols separately, the re...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Dry and pulverize fresh rattan tea stems and leaves to obtain 500g of raw material powder, add 6L of distilled water, heat to 95°C and extract for 30min, filter with 100-mesh double-layer filter cloth while hot, then centrifuge while hot (4000rpm, 15min), collect For the supernatant I, add 4L of distilled water to the filter residue, heat to 95°C and repeat the extraction once, combine the supernatant I; concentrate the supernatant I under reduced pressure to 1 / 3 volume, fully cool, and stand at 4°C After 12 hours of crystallization, centrifuge (4000rpm, 15min) to collect the precipitate A and the supernatant II respectively; the precipitate A is recrystallized by adding water twice, and then the crystals are vacuum-dried at 40°C to obtain dihydromyricetin, the product Yield can reach 11% (dihydromyricetin is used as standard sample, aluminum nitrate-sodium nitrite colorimetric method is measured, and product dihydromyricetin content is more than or equal to 85%); Supern...

Embodiment 2

[0012] Dry and pulverize fresh rattan tea stems and leaves to obtain 1000g of raw material powder, add 12L of distilled water, heat to 95°C for extraction for 30min, filter with 100-mesh double-layer filter cloth while hot, then centrifuge while hot (4000rpm, 15min), collect For the supernatant I, add 8L of distilled water to the filter residue, heat to 95°C and repeat the extraction once, combine the supernatant I; concentrate the supernatant I under reduced pressure to 1 / 3 volume, fully cool, and stand at 4°C After 12 hours of crystallization, centrifuge (4000rpm, 15min) to collect the precipitate A and the supernatant II respectively; the precipitate A is recrystallized by adding water twice, and then the crystals are vacuum-dried at 40°C to obtain the dihydromyricetin product. The product yield can reach 11% (dihydromyricetin is used as a standard sample, and the aluminum nitrate-sodium nitrite colorimetric method determines that the product dihydromyricetin content is ≥ 85...

Embodiment 3

[0014] Dried and crushed fresh vine tea stems and leaves as raw materials for extraction; add 1500g of raw material powder, add 18L of distilled water, heat to 95°C and extract for 30min, filter with 100-mesh double-layer filter cloth while hot, and then centrifuge while hot (4000rpm, 15min) , collect the supernatant I, add 12L of distilled water to the filter residue, heat to 95°C and repeat the extraction once, combine the supernatant I; concentrate the supernatant I under reduced pressure to 1 / 3 volume, fully cool, at 4°C Stand still for 12 hours, after crystallization, centrifuge (4000rpm, 15min), collect precipitate A and supernatant II respectively; add water to precipitate A twice and recrystallize, then dry the crystals in vacuum at 40°C to obtain dihydromyricetin , the product yield can reach 11% (dihydromyricetin is used as a standard sample, measured by aluminum nitrate-sodium nitrite colorimetric method, product dihydromyricetin content ≥ 85%); the supernatant II is...

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Abstract

The invention relates to a method for extracting dihydromyricetin, ampelopsis grossedentata amylase and ampelopsis grossedentata polyphenol from ampelopsis grossedentata. The method comprises the following processing steps of: (a) crushing raw materials: drying and crushing fresh ampelopsis grossedentata stem leaves; (b) leaching: extracting with hot water and centrifugally collecting supernatant fluid; (c) concentrating: decompressing and concentrating the supernatant fluid I, cooling, standing still and centrifugally collecting precipitate and supernatant fluid II; (d) extracting dihydromyricetin: recrystallizing and drying the precipitate in vacuum to obtain dihydromyricetin; (e) alcoholizing: decompressing and concentrating the supernatant fluid II, depositing in alcohol and centrifugally collecting a precipitate and supernatant fluid III; (f) extracting crude polysaccharides: purifying the precipitate, drying in vacuum or spraying and drying to obtain ampelopsis grossedentata amylase; and (g) extracting ampelopsis grossedentata polyphenol: decompressing and concentrating the supernatant fluid III, extracting with ethyl acetate, concentrating and drying to obtain ampelopsis grossedentata polyphenol or absorbing with AB-8 resin, eluting with distilled water, eluting with ethanol and collecting eluate, concentrating and drying in vacuum to obtain ampelopsis grossedentata polyphenol. The method has the advantages of low cost, higher product yield and environmental protection, is suitable for industrial continuous production and can comprehensively develop and utilize ampelopsis grossedentata resources and improve the economic benefits.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, in particular to a method for extracting dihydromyricetin, vine tea polysaccharides and vine tea polyphenols from vine tea. Background technique [0002] Vine tea is a substitute tea made from the young stems and leaves of Ampelopsis-grossedentata (Hand-Mazz) W.T.Wang, a plant of the genus Ampelopsis-grossedentata (Hand-Mazz) W.T.Wang. The plant is mainly distributed in Hubei, Hunan, Guangdong, Guangxi, Jiangxi, Fujian and other provinces, and is rich in resources. The traditional application history and modern pharmacological studies of vine tea show that vine tea is rich in flavonoids (mainly dihydromyricetin), vine tea polysaccharides, vine tea polyphenols and other components, and has strong antioxidant, Hypoglycemic and other biological activities. At present, the extraction and bioactivity research of the bioactive components of vine tea mainly focus on the flavonoids o...

Claims

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Application Information

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IPC IPC(8): C08B37/00C07D311/40C07G99/00C07D311/32
Inventor 罗祖友郑小江李世荣向东山刘金龙程超唐巧玉周大寨肖强向班贵赵玉宏武芸肖浩
Owner HUBEI UNIV FOR NATITIES
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