Serum-free culture medium for umbilical cord mesenchymal stem cells, as well as preparation method and application thereof

Abstract

The invention provides a serum-free culture medium for umbilical cord mesenchymal stem cells. The serum-free culture medium is characterized by comprising a DMEM low-sugar culture medium, transferrin, serum albumin, insulin, platelet-derived growth factors, epidermal growth factors, transforming growth factors, beta-mercaptoethanol, dihydromyricetin and catechin. The invention belongs to the technical field of stem cell culture. The serum-free culture medium for umbilical cord mesenchymal stem cells, provided by the invention, can obviously promote the adherence performance and multiplication rate of the umbilical cord mesenchymal stem cells, is beneficial to the multiplication of the umbilical cord mesenchymal stem cells and the maintenance of features of the stem cells, is excellent in adipogenesis and osteogenic induction differentiation potential, is relatively simple in component of the culture medium and is relatively low in cost.

Description

technical field [0001] The invention belongs to the technical field of stem cell culture, and in particular relates to a serum-free culture solution of umbilical cord mesenchymal stem cells and a preparation method and application thereof. Background technique [0002] Human umbilical cord mesenchymal stem cells (hUCMSCs) are derived from umbilical cord Walter's jelly, and can differentiate into different tissue cell types derived from three germ layers under the regulation of different growth factors. Human umbilical cord mesenchymal stem cells have the advantages of convenient material collection, wide range of sources, and no ethical restrictions. They do not express major histocompatibility class II antigens, but only slightly express major histocompatibility class I antigens. They have low immunogenicity and transplant rejection It is small and has immunoregulatory effects. The trophic factors secreted by human umbilical cord mesenchymal stem cells after transplantation...

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Problems solved by technology

[0004] Chinese patent application CN 105420182 discloses a serum-free umbilical cord mesenchymal stem cell culture medium, including DMEM medium, basic fibroblast growth factor, epidermal growth factor, insulin, leukemia inhibitory factor, L-glutamine, 2- Mercaptoethanol, sodium selenite, and fibronectin, etc., achieved better adhesion performance by adding higher levels of fibronectin, but the cell proliferation rate was slower

[0005] Chinese patent application CN 104877963 discloses a serum-free culture medium for human umbilical cord mesenchymal stem cells, including IMDM basal medium, and the following components: recombinant human insulin, recombinant transferrin, vitamin C, human serum albumin, Fibroblast growth factor, platelet-derived growth factor, epidermal growth factor, insulin-like growth factor, transforming growth factor, fibronectin, fetuin, and beta-cytokinin, human umbilical cord interstitial The adherence performance of mesenchymal stem cells, the stem cell characteristics of human umbilical cord mesenchymal stem cells are maintained by adding β-cell factor, the components are more complex and the cost is more expensive

[0006] At present, there are some commercially available umbilical cord mesenchymal stem cell serum-free medium, such as Gibco, Saiye Biotechnology and other companies' umbilical cord mesenchymal stem cell serum-free medium, but there are disadvantages such as high price and unsatisfactory adherence performance. Poor wall performance will affect a series of responses such as proliferation and differentiation of umbilical cord mesenchymal stem cells

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