Bacteriostatic gynecological gel and preparation method thereof

Abstract

The invention discloses antibacterial gynecological gel and a preparation method thereof, and relates to the field of gynecological products, arginine powder and sorbitol are dissolved in water, a hydrothermal reaction is performed at 130-160 DEG C, cooling is performed to obtain an arginine carbon quantum dot solution, then modified hydrogel and a plant extract are added, vacuum stirring and uniform mixing are performed, ultraviolet irradiation is started while stirring, standing is performed, and the antibacterial gynecological gel is obtained. The bacteriostatic gynecological gel is obtained. The adopted gel matrix releases arginine carbon quantum dots in a weak acid environment, and is compounded with active substances, especially flavonoid substances, in the plant extracting solution, so that a better synergistic antibacterial effect is achieved. Sensitive response of the carbon quantum dots to illumination is combined, so that the carbon quantum dots can be attached to a gel matrix more easily; meanwhile, polar groups, especially oxygen-containing groups, are introduced to the surface of the gel, so that the viscosity and the adhesiveness of the gel material are greatly improved, and the affected part can be better treated.

Description

Technical Field

[0001] The invention relates to the field of gynecological products, and in particular to an antibacterial gynecological gel and a preparation method thereof. Background Art

[0002] Gynecological antibacterial gel is a gynecological medicine, a gel, used for the treatment of various gynecological vaginitis and cervicitis, including: bacterial vaginitis, trichomonas vaginitis, mixed vaginitis, gonococcal infection, chronic cervicitis. It has anti-inflammatory, bactericidal and antipruritic effects. It has a significant inhibitory effect on the growth of pathogenic microorganisms such as Staphylococcus aureus, Escherichia coli, gonococci, Candida albicans, fungi, yeasts, etc.

[0003] The current gynecological gel can only stay on the mucous membrane for a short time when used, which results in the inability to completely kill pathogens after use, resulting in a decrease in its effectiveness. Summary of the invention

[0004] The purpose of the present invention is to solve at least one of the technical problems existing in the prior art and to provide an antibacterial gynecological gel and a preparation method thereof.

[0005] The technical solution of the present invention is as follows:

[0006] A method for preparing an antibacterial gynecological gel comprises the following steps: dissolving arginine powder and sorbitol in water, carrying out a hydrothermal reaction at 130-160° C., cooling to obtain an arginine carbon quantum dot solution, then adding a modified hydrogel and a plant extract, stirring and mixing in a vacuum, starting ultraviolet light irradiation while stirring, and standing to obtain the antibacterial gynecological gel.

[0007] As a preferred embodiment of the present invention, the preparation method of the plant extract is as follows:

[0008] Grind 3-8 parts of rose, 15-25 parts of sophora flavescens, 15-20 parts of phellodendron, 9-15 parts of angelica dahurica, 7-14 parts of cnidium monnieri, 3-11 parts of aloe and 1-5 parts of angelica sinensis, add 1-10 parts of quercetin to obtain a powder raw material, then add 8-10 times the mass of water to decoct, decoct 2-3 times, decoct for 1-1.5 hours each time, combine the decoctions, concentrate, and obtain a plant extract.

[0009] As a preferred embodiment of the present invention, the mass ratio of the arginine powder to sorbitol is 1:2-5.

[0010] As a preferred embodiment of the present invention, the concentration of the arginine carbon quantum dot solution is 0.002-0.01 wt %.

[0011] As a preferred embodiment of the present invention, the volume ratio of the arginine carbon quantum dot solution, the modified hydrogel and the plant extract is 1-3:10-20:50-200.

[0012] As a preferred embodiment of the present invention, the preparation method of the modified hydrogel is as follows:

[0013] The cellulose is added into an alkali solution, then cooled to -10°C to -2°C for swelling treatment, then heated to 30-50°C, mixed with a silane coupling agent for coupling reaction, and then the reaction solution is oxidized to obtain.

[0014] As a preferred embodiment of the present invention, the cellulose is a mixture of carboxymethyl cellulose and hydroxypropyl cellulose in a mass ratio of 1:1-3.

[0015] As a preferred embodiment of the present invention, the alkali solution is a sodium hydroxide solution with a concentration of 12-18wt%.

[0016] As a preferred embodiment of the present invention, the oxidation treatment specifically comprises: adding an oxidant to the reaction solution and intermittently performing microwave radiation.

[0017] An antibacterial gynecological gel, prepared by any of the above preparation methods

[0018] The beneficial effects of the present invention are:

[0019] Since gynecological gels are often used in the vagina, which is a weakly acidic environment, the gel matrix used in the present invention releases arginine carbon quantum dots in a weakly acidic environment and combines with active substances in plant extracts, especially flavonoids, to achieve a better synergistic antibacterial effect. Combined with the sensitive response of carbon quantum dots to light, it is easier to attach to the gel matrix; at the same time, the introduction of oxygen-containing polar groups on the surface of the gel greatly improves the viscosity and adhesion of the gel material, allowing the antibacterial active ingredients to stay better in the affected area and improve the therapeutic effect.

[0020] The present invention can also be used in combination with products for the treatment of chronic cervicitis and HPV, which can effectively improve clinical abnormal symptoms, increase the HPV negative conversion rate and shorten the conversion time, and reduce the level of inflammatory cytokines. In addition, the gynecological gel provided can improve the condition while promoting the recovery of the patient's vaginal microecological environment, thereby improving the body's immunity and the self-cleaning ability of the vagina. DETAILED DESCRIPTION

[0021] The embodiments of the present invention are described in detail below. The embodiments described below are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention. If no specific techniques or conditions are specified in the embodiments, the techniques or conditions described in the literature in this area or the product specifications are used. The reagents or instruments used that do not specify the manufacturer are all conventional products that can be obtained commercially.

[0022] Example 1

[0023] A method for preparing an antibacterial gynecological gel comprises the following steps: dissolving arginine powder and sorbitol in water, performing a hydrothermal reaction at 150° C., cooling to obtain an arginine carbon quantum dot solution, then adding a modified hydrogel and a plant extract, stirring and mixing in a vacuum, starting 365 nm ultraviolet light irradiation while stirring, and standing to obtain the antibacterial gynecological gel.

[0024] The preparation method of the plant extract is as follows:

[0025] Grind 5 parts of rose, 15 parts of sophora flavescens, 18 parts of phellodendron, 12 parts of angelica dahurica, 8 parts of cnidium monnieri, 10 parts of aloe and 4 parts of angelica sinensis, add 5 parts of quercetin to obtain a powder raw material, then add 9 times the mass of water to decoct, decoct for 3 times, decoct for 1 hour each time, combine the decoctions, concentrate, and obtain a plant extract.

[0026] The mass ratio of the arginine powder to sorbitol is 1:3.

[0027] The concentration of the arginine carbon quantum dot solution is 0.002 wt %.

[0028] The volume ratio of the arginine carbon quantum dot solution, the modified hydrogel and the plant extract is 1:13:100.

[0029] The preparation method of the modified hydrogel is as follows:

[0030] The cellulose is added into an alkali solution, then cooled to -8°C for swelling treatment, then heated to 40°C, mixed with 3-mercaptopropyltrimethoxysilane for coupling reaction, and then the reaction solution is oxidized to obtain.

[0031] The cellulose is a mixture of carboxymethyl cellulose and hydroxypropyl cellulose in a mass ratio of 1:2.

[0032] The alkali solution is a sodium hydroxide solution with a concentration of 14wt%.

[0033] The oxidation treatment specifically includes: adding an oxidant (2 mol / L hydrogen peroxide) to the reaction solution and reacting for 5 hours, intermittently performing 250 MHz microwave irradiation, each irradiation for 30 minutes, with an interval of 5 minutes between adjacent irradiations.

[0034] Example 2

[0035] A method for preparing an antibacterial gynecological gel comprises dissolving arginine powder and sorbitol in water, performing a hydrothermal reaction at 150° C., cooling to obtain an arginine carbon quantum dot solution, then adding a modified hydrogel and a plant extract, stirring and mixing in a vacuum, starting ultraviolet light irradiation while stirring, and standing to obtain the antibacterial gynecological gel.

[0036] The preparation method of the plant extract is as follows:

[0037] Grind 5 parts of rose, 15 parts of sophora flavescens, 18 parts of phellodendron, 12 parts of angelica dahurica, 8 parts of cnidium monnieri, 10 parts of aloe and 4 parts of angelica sinensis, add 5 parts of quercetin to obtain a powder raw material, then add 9 times the mass of water to decoct, decoct for 3 times, decoct for 1 hour each time, combine the decoctions, concentrate, and obtain a plant extract.

[0038] The mass ratio of the arginine powder to sorbitol is 1:3.

[0039] The concentration of the arginine carbon quantum dot solution is 0.004 wt %.

[0040] The volume ratio of the arginine carbon quantum dot solution, the modified hydrogel and the plant extract is 1:13:100.

[0041] The preparation method of the modified hydrogel is as follows:

[0042] The cellulose is added into an alkali solution, then cooled to -8°C for swelling treatment, then heated to 40°C, mixed with 3-mercaptopropyltrimethoxysilane for coupling reaction, and then the reaction solution is oxidized to obtain.

[0043] The cellulose is a mixture of carboxymethyl cellulose and hydroxypropyl cellulose in a mass ratio of 1:2.

[0044] The alkali solution is a sodium hydroxide solution with a concentration of 14wt%.

[0045] The oxidation treatment specifically includes: adding an oxidant (2 mol / L hydrogen peroxide) to the reaction solution and reacting for 5 hours, intermittently performing 250 MHz microwave irradiation, each irradiation for 30 minutes, with an interval of 5 minutes between adjacent irradiations.

[0046] Example 3

[0047] A method for preparing an antibacterial gynecological gel comprises dissolving arginine powder and sorbitol in water, performing a hydrothermal reaction at 150° C., cooling to obtain an arginine carbon quantum dot solution, then adding a modified hydrogel and a plant extract, stirring and mixing in a vacuum, starting ultraviolet light irradiation while stirring, and standing to obtain the antibacterial gynecological gel.

[0048] The preparation method of the plant extract is as follows:

[0049] Grind 5 parts of rose, 15 parts of sophora flavescens, 18 parts of phellodendron, 12 parts of angelica dahurica, 8 parts of cnidium monnieri, 10 parts of aloe and 4 parts of angelica sinensis, add 5 parts of quercetin to obtain a powder raw material, then add 9 times the mass of water to decoct, decoct for 3 times, decoct for 1 hour each time, combine the decoctions, concentrate, and obtain a plant extract.

[0050] The mass ratio of the arginine powder to sorbitol is 1:3.

[0051] The concentration of the arginine carbon quantum dot solution is 0.008 wt %.

[0052] The volume ratio of the arginine carbon quantum dot solution, the modified hydrogel and the plant extract is 1:13:100.

[0053] The preparation method of the modified hydrogel is as follows:

[0054] The cellulose is added into an alkali solution, then cooled to -18°C for swelling treatment, then heated to 40°C, mixed with 3-mercaptopropyltrimethoxysilane for coupling reaction, and then the reaction solution is oxidized to obtain.

[0055] The cellulose is a mixture of carboxymethyl cellulose and hydroxypropyl cellulose in a mass ratio of 1:2.

[0056] The alkali solution is a sodium hydroxide solution with a concentration of 14wt%.

[0057] The oxidation treatment specifically includes: adding an oxidant (2 mol / L hydrogen peroxide) to the reaction solution and reacting for 5 hours, intermittently performing 250 MHz microwave irradiation, each irradiation for 30 minutes, with an interval of 5 minutes between adjacent irradiations.

[0058] Example 4

[0059] A method for preparing an antibacterial gynecological gel comprises dissolving arginine powder and sorbitol in water, performing a hydrothermal reaction at 150° C., cooling to obtain an arginine carbon quantum dot solution, then adding a modified hydrogel and a plant extract, stirring and mixing in a vacuum, starting ultraviolet light irradiation while stirring, and standing to obtain the antibacterial gynecological gel.

[0060] The preparation method of the plant extract is as follows:

[0061] Grind 5 parts of rose, 15 parts of sophora flavescens, 18 parts of phellodendron, 12 parts of angelica dahurica, 8 parts of cnidium monnieri, 10 parts of aloe and 4 parts of angelica sinensis, add 5 parts of quercetin to obtain a powder raw material, then add 9 times the mass of water to decoct, decoct for 3 times, decoct for 1 hour each time, combine the decoctions, concentrate, and obtain a plant extract.

[0062] The mass ratio of the arginine powder to sorbitol is 1:3.

[0063] The concentration of the arginine carbon quantum dot solution is 0.01 wt %.

[0064] The volume ratio of the arginine carbon quantum dot solution, the modified hydrogel and the plant extract is 1:13:100.

[0065] The preparation method of the modified hydrogel is as follows:

[0066] The cellulose is added into an alkali solution, then cooled to -8°C for swelling treatment, then heated to 40°C, mixed with 3-mercaptopropyltrimethoxysilane for coupling reaction, and then the reaction solution is oxidized to obtain.

[0067] The cellulose is a mixture of carboxymethyl cellulose and hydroxypropyl cellulose in a mass ratio of 1:2.

[0068] The alkali solution is a sodium hydroxide solution with a concentration of 14wt%.

[0069] The oxidation treatment specifically includes: adding an oxidant (2 mol / L hydrogen peroxide) to the reaction solution and reacting for 5 hours, intermittently performing 250 MHz microwave irradiation, each irradiation for 30 minutes, with an interval of 5 minutes between adjacent irradiations.

[0070] Example 5

[0071] A method for preparing an antibacterial gynecological gel comprises dissolving arginine powder and sorbitol in water, performing a hydrothermal reaction at 150° C., cooling to obtain an arginine carbon quantum dot solution, then adding a modified hydrogel and a plant extract, stirring and mixing in a vacuum, starting ultraviolet light irradiation while stirring, and standing to obtain the antibacterial gynecological gel.

[0072] The preparation method of the plant extract is as follows:

[0073] Grind 5 parts of rose, 15 parts of sophora flavescens, 18 parts of phellodendron, 12 parts of angelica dahurica, 8 parts of cnidium monnieri, 10 parts of aloe and 4 parts of angelica sinensis, add 5 parts of quercetin to obtain a powder raw material, then add 9 times the mass of water to decoct, decoct for 3 times, decoct for 1 hour each time, combine the decoctions, concentrate, and obtain a plant extract.

[0074] The mass ratio of the arginine powder to sorbitol is 1:5.

[0075] The concentration of the arginine carbon quantum dot solution is 0.004 wt %.

[0076] The volume ratio of the arginine carbon quantum dot solution, the modified hydrogel and the plant extract is 1:20:200.

[0077] The preparation method of the modified hydrogel is as follows:

[0078] The cellulose is added into an alkali solution, then cooled to -8°C for swelling treatment, then heated to 40°C, mixed with 3-mercaptopropyltrimethoxysilane for coupling reaction, and then the reaction solution is oxidized to obtain.

[0079] The cellulose is a mixture of carboxymethyl cellulose and hydroxypropyl cellulose in a mass ratio of 1:3.

[0080] The alkali solution is a sodium hydroxide solution with a concentration of 14wt%.

[0081] The oxidation treatment specifically includes: adding an oxidant (2 mol / L hydrogen peroxide) to the reaction solution and reacting for 5 hours, intermittently performing 250 MHz microwave irradiation, each irradiation for 30 minutes, with an interval of 5 minutes between adjacent irradiations.

[0082] Comparative Example 1

[0083] This comparative example is different from Example 2 in that arginine carbon quantum dots are not added. Specifically, the modified hydrogel and the plant extract are mixed by vacuum stirring, and ultraviolet light irradiation is started while stirring, and the mixture is allowed to stand to obtain an antibacterial gynecological gel. The rest is the same as Example 2.

[0084] Comparative Example 2

[0085] This comparative example is different from Example 2 in that ultraviolet light irradiation is not started. Specifically, a method for preparing an antibacterial gynecological gel is provided, wherein arginine powder and sorbitol are dissolved in water, subjected to a hydrothermal reaction at 150° C., cooled to obtain an arginine carbon quantum dot solution, and then the modified hydrogel and the plant extract are added, the mixture is mixed under vacuum stirring, and the mixture is allowed to stand to obtain an antibacterial gynecological gel. The rest is the same as Example 2.

[0086] Comparative Example 3

[0087] The difference between this comparative example and Example 2 is that the gel is not subjected to oxidation treatment. Specifically, cellulose is added to an alkali solution, then cooled to -8°C for swelling treatment, then heated to 40°C, mixed with a silane coupling agent for coupling reaction, and the rest is the same as Example 2.

[0088] The above embodiments and comparative examples were subjected to performance tests, and the test results are shown in Table 1.

[0089] The test method refers to the method in Appendix C of GB15979-2002 "Hygiene Standard for Disposable Sanitary Products".

[0090] Table 1 Performance test results of embodiments and comparative examples

[0091]

[0092]

[0093] As can be seen from the table above, the performance of the embodiment is better than that of the comparative example. The main reasons may be as follows: from the analysis of comparative example 1, it can be seen that the gel matrix used in the embodiment releases arginine carbon quantum dots in a weakly acidic environment, and is compounded with the active substances in the plant extract, especially flavonoids, to achieve a better synergistic antibacterial effect. From the analysis of comparative example 2, it can be seen that the embodiment uses ultraviolet light irradiation, combined with the sensitive response of carbon quantum dots to light, making it easier to attach to the gel matrix; from the analysis of comparative example 3, it can be seen that the embodiment introduces oxygen-containing polar groups on the surface of the gel, which greatly improves the viscosity and adhesion of the gel material, allowing the antibacterial active ingredients to stay better in the affected area and improve the therapeutic effect.

[0094] In addition, the present invention can also be used in combination with products for the treatment of chronic cervicitis and HPV, which can effectively improve clinical abnormal symptoms, increase the HPV negative conversion rate and shorten the conversion time, and reduce the level of inflammatory cytokines. The gynecological gel provided by the present invention can improve the condition while promoting the recovery of the patient's vaginal microecological environment, thereby improving the body's immunity and the self-cleaning ability of the vagina.

[0095] The above-mentioned embodiments only express the preferred implementation modes of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that, for ordinary technicians in this field, various other corresponding changes and deformations can be made according to the technical solutions and concepts described above, and all these changes and deformations should fall within the scope of protection of the claims of the present invention.

Claims

1. A method for preparing an antibacterial gynecological gel, characterized in that: Arginine powder and sorbitol are dissolved in water, and a hydrothermal reaction is carried out at 130-160° C. The solution is cooled to obtain an arginine carbon quantum dot solution. The modified hydrogel and plant extract are then added and mixed under vacuum stirring. While stirring, ultraviolet light irradiation is initiated and the solution is allowed to stand to obtain an antibacterial gynecological gel.

2. The method for preparing an antibacterial gynecological gel according to claim 1, characterized in that: The preparation method of the plant extract is as follows: Grind 3-8 parts of rose, 15-25 parts of sophora flavescens, 15-20 parts of phellodendron, 9-15 parts of angelica dahurica, 7-14 parts of cnidium monnieri, 3-11 parts of aloe and 1-5 parts of angelica sinensis, add 1-10 parts of quercetin to obtain a powder raw material, then add 8-10 times the mass of water to decoct, decoct 2-3 times, decoct for 1-1.5 hours each time, combine the decoctions, concentrate, and obtain a plant extract.

3. The method for preparing an antibacterial gynecological gel according to claim 1, characterized in that: The mass ratio of the arginine powder to sorbitol is 1:2-5.

4. The method for preparing an antibacterial gynecological gel according to claim 1, characterized in that: The concentration of the arginine carbon quantum dot solution is 0.002-0.01 wt %.

5. The method for preparing an antibacterial gynecological gel according to claim 1, characterized in that: The volume ratio of the arginine carbon quantum dot solution, the modified hydrogel and the plant extract is 1-3:10-20:50-200.

6. The method for preparing an antibacterial gynecological gel according to claim 1, characterized in that: The preparation method of the modified hydrogel is as follows: The cellulose is added into an alkali solution, then cooled to -10°C to -2°C for swelling treatment, then heated to 30-50°C, mixed with a silane coupling agent for coupling reaction, and then the reaction solution is oxidized to obtain.

7. The method for preparing an antibacterial gynecological gel according to claim 6, characterized in that: The cellulose is a mixture of carboxymethyl cellulose and hydroxypropyl cellulose in a mass ratio of 1:1-3.

8. The method for preparing an antibacterial gynecological gel according to claim 6, characterized in that: The alkali solution is a sodium hydroxide solution with a concentration of 12-18wt%.

9. The method for preparing an antibacterial gynecological gel according to claim 6, characterized in that: The oxidation treatment specifically includes: adding an oxidant to the reaction solution and intermittently performing microwave radiation.

10. An antibacterial gynecological gel, characterized in that: The method is prepared by any one of claims 1 to 9.

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