65results about How to "Rapid Analysis Detection" patented technology

The invention relates to a method for detecting organic pollutants by virtue of combination of solid phase micro-extraction and surface enhancement Raman. The method comprises the following steps: with a solid phase micro-extraction probe as a surface enhancement Raman active substrate, extracting for 3-10 hours in the to-be-detected environment under the stirring condition with the temperature of 20-30 DEG C, then putting the solid phase micro-extraction probe on a Raman detection platform, irradiating the solid phase micro-extraction probe by using laser to obtain Raman characteristic peaks of the to-be-detected organic pollutants, and analyzing the detected organic pollutants according to the Raman characteristic peaks. The method for detecting the organic pollutants is integrated with the advantages of the solid phase micro-extraction technology and the surface enhancement Raman technology; the organic pollutants in the environment can be quickly analyzed and detected; the sameness analysis of polyaromatic hydrocarbon can be realized; polyaromatic hydrocarbon mixtures can be detected and analyzed.

The invention belongs to the technical field of food, and more specifically relates to a color/fluorescence double signal visible rapid nitrite detection method, and applications thereof. The color/fluorescence double signal visible rapid nitrite detection method comprises following steps: firstly, a carbon dot-neutral red composite system is prepared, wherein carbon dot synthesis and constructionof the carbon dot-neutral red composite system are carried out; a common filter paper is immersed in the carbon dot-neutral red composite system so as to obtain a carbon dot-neutral red composite system detection test paper; a sodium nitrite standard solution is prepared; a color standard colourimetric card and a fluorescence standard colourimetric card of nitrites are prepared; a software is adopted to extract the RGB values of each test paper, a matrix is constructed, and a multivariate linear regression model is constructed; and the content of nitrites in food to be detected is detected. The carbon dot-neutral red composite system possess color/fluorescence double response capacity on nitrites; detection operation steps are simple; the visible results are clear, visual, and accurate; the detection limit is lower; and it is promising for the color/fluorescence double signal visible rapid nitrite detection method to be used in large-scale food nitrite content detection.

The invention discloses a method for determining glucose based on a paper-based electrochemical device. The method comprises the following steps that 1, filter paper serves as a detection platform of a paper-based substrate, an electrode is manually drawn with a pencil, and graphite serves as an electrode material, wherein the detection platform comprises two areas, namely a detection area and an enzyme area, each area is provided with a round hydrophilic area, and the part except the hydrophilic area is a hydrophobic area; 2, according to the principle of glucose oxidase oxidizing reaction, ferrocenecarboxylic acid serves as an electronic mediator, glucose is determined by adopting an amperometric method, and the detection platform can sensitively determine the glucose in the presence of interferent; a sample to be detected is added to the detection platform, and the actual blood sugar concentration of the sample can be detected. The paper-based electrochemical system adopted in the method is the novel electrochemical device, the electrochemical device is simple in manufacture and low in cost and can be used in lots of application fields including portable and disposable analytical equipment for clinical diagnosis, food quality control, environment monitoring and the like.

The invention provides a chiral chromatographic separation and analysis method of dihydromyricetin enantiomer. The method comprises the following steps: dissolving a dihydromyricetin sample into the mobile phase, taking polysaccharide derivatives as the chiral stationary phase, and carrying out liquid chromatogram (LC) separation and analysis; wherein the mobile phase is a mixed solution of n-hexane and lower alcohols, and the polysaccharide derivative chiral stationary phase is one of stationary phases, which are individually covered by tri(3,5-dimethylphenylcarbamyl) straight-chain starch, tri((S)-(alpha)- phenylethylcarbamyl) straight-chain starch, tri(3,5- dimethylphenylcarbamyl)cellulose and tri(4- methylphenylcarbamyl)cellulose. According to the provided chiral separation and analysis method, the maximal separation degree of dihydromyricetin enantiomer is 1.95, and the maximal selection factor is 1.36.

The invention discloses an application of an electrochemiluminescence biosensor based on cytosine (C)-containing cyclic DNA sequences where in-situ reduced Ag nano-clusters are enriched as signal probes and adopting dual amplification strategy to detection of target thrombin. According to the technical scheme, DNA cleavage enzyme with hairpin DNA recognition capability and catalysis effect is designed, when target thrombin exists, hairpin DNA is opened, and substrate DNA is cleaved under the action of Zn<2+>; a large quantity of cytosine (C)-containing cyclic DNA sequences are aggregated through HCR (hybridization chain reaction), in-situ reduction of AgNO3 is performed on the electrode surface by NaBH4, a large quantity of Ag nano-clusters are formed, and the electrochemiluminescence biosensor with dual amplification effect is prepared. The sensor is subjected to luminescence detection, and a linear relation is formed between light-emitting signals and concentration of a to-be-testedsample. The Ag nano-cluster signal probes and the DNA dual amplification technology are combined for rapid and high-sensitivity detection of thrombin, and the electrochemiluminescence biosensor has great application potential in early clinical analysis and detection.

The invention belongs to the technical field of a biosensor and discloses a preparation method of the electrochemiluminescence biosensor for detecting mercury ions. Carbon nitride quantum dots are taken as a new-type co-reacant, so an electrochemiluminescence signal of a luminophor [Ru(dcbpy)<3><2+>] can be enhanced. In order to realize specific detection for the mercury ions, DNA1 and DNA2 rich in T bases are imported, and the DNA1 and the DNA2 are connected with the luminophor and the co-reacant. Pairing bonding can be carried out on the DNA1 and the DNA2 through utilization of the mercury ions. Distance between the luminophor and the co-reacant is closer, so the electrochemiluminescence signal is further enhanced. According to the constructed electrochemiluminescence biosensor, a detection limit for the mercury ions is 3.3x10 <-15>mol.L<-1>. The electrochemiluminescence biosensor provided by the invention is low in cost, high in sensitivity and good in selectivity and is applicableto practical application detection of mercury ion content in surface water.

The invention provides an OTA (Ochratoxin A) chemiluminescence detection method based on a label-free aptasensor. According to the technical scheme, the OTA chemiluminescence detection method comprises: fixing an aptamer capturing probe on the surface of a magnetic microsphere; combining the capturing probe and OTA with a competition relationship of an OTA aptamer respectively; obtaining a quantitative relationship between the quantity of magnetic microsphere surface detection probes and the concentration of OTA to be detected based on the fact that the specific combining capability of the aptamer is stronger than the binding force of complementary strands; finally, carrying out chemiluminescence detection on the aptasensor. A chemiluminescence detection mechanism of the OTA chemiluminescence detection method is realized based on a principle that a guanine base (G) on an OTA aptamer strand and a chemiluminescence reagent phenylglyoxal (PG) are subjected to an instantaneous derivative reaction to generate chemiluminescence, so that a labeling link of other chemiluminescence markers is omitted; operation steps are simplified on the basis of guanrateeing the sensitivity and the detection time is shortened; a chemiluminescence anslysis method has the advantages of wide linear range, simple equipment and easiness of operation, so that full-automatic detection is hopeful to realize.

The invention relates to an atmospheric pressure photoionization mass spectrum device for analyzing solid fuel pyrolysis on line. The device comprises a pyrolyzing furnace, an auxiliary volatile solvent introduction mechanism, an atmospheric pressure photoionization source and a mass spectrometer. With the increasingly exhausted fossil fuel, a process of preparing various hydrocarbon fuels and petrochemical materials through solid fuel pyrolysis is gradually concerned by people. According to the invention, the species distribution and generation mechanism of solid fuel pyrolysis products such as solid wastes (such as polypropylene), biomass, coal and oil shale can be accurately and rapidly analyzed on line in real time, people can be helped to well master the internal rules of pyrolysis, effective resource utilization is comprehensively realized, and the device has great significance on sustainable development of China.

The invention discloses a trace substance detector with two fluorescence emission surfaces, and a detection method, and relates to an instrument used for detecting toxic and hazardous trace substances contained in environments, food, health care products, traditional Chinese medicines, cosmetics and the like in the technical field of spectrographic detection. The detector comprises an excitation unit, a first detection unit and a second detection unit, wherein the first detection unit is parallel to the second detection unit; the excitation unit is perpendicular to the first detection unit. Compared with a dual-waveband fluorescence intensity ratio detection system with a single fluorescence emission surface, the detector is not provided with a dichroic mirror or a fluorescence condensing lens on the basis of keeping the detection stability and an accuracy rate, so that a fluorescence loss is low; an optical path is short; the sensitivity is higher; a detection limit is lower. Under the equivalent condition of copper ion detection, by comparison of an optical system with two fluorescence emission surfaces and an optical system with a single fluorescence emission surface, the fluorescence detection strength is improved by 1.2 times and the detection limit is reduced from 0.03 mg/kg to 0.015 mg/kg. The detector is smaller in size and simple to operate, and can be used for on-site real-time quantitative rapid analysis and detection.

The invention discloses a method for analyzing and detecting trace metal ion impurities in dimethyl diallyl ammonium chloride (DMDAAC) monomers. According to the method, an organic solvent chelating extraction-flame atomic absorption spectroscopy method is adopted to measure the contents of multiple trace metal ions in DMDAAC monomers of a DMDAAC monomer solution with a high salt content, and avoids the difficult operation and result errors caused by direct injection of a sample with a high salt content. By utilizing the synergistic effect of a complexing agent, three metal ions (Fe, Cu, and Ni) can be complexed, extracted, separated, analyzed, and tested at the same time. The provided method has the advantages that the operation is simple and convenient, the analysis precision is high, and the contents of several trace metal ion impurities in DMDAAC monomers can be accurately, rapidly, and reliably analyzed and detected. The provide method has an important scientific meaning and industrial practical value for industrial manufacturing of high purity DMDAAC monomers, establishment of national/international standards of DMDAAC monomers, and research on the activity of DMDAAC monomers in polymerization reactions.

The invention discloses an ISSR (inter-simple sequence repeat) primer and a reaction system for identifying Dendrobium officinale Kimura et Migo and Dendrobium huoshanense C. Z. Tang et S. J. Cheng. The nucleotide sequence of the primer is as shown in SEQ ID NO.1-6, and DNA (deoxyribonucleic acid) of the Dendrobium officinale Kimura et Migo and the Dendrobium huoshanense C. Z. Tang et S. J. Chengis amplified by the aid of the primer and an optimized reaction system, so that identification can be completed. The primer and a method are simple, time and cost are saved, and the primer has good application value to identification, genetic relationship analysis and the like of the Dendrobium officinale Kimura et Migo and the Dendrobium huoshanense C. Z. Tang et S. J. Cheng.

The invention discloses a mobile rice leaf nitrogen content detecting and grading method based on machine vision. By using analysis on the correlation between optical indexes provided by a digital image and a rice leaf nitrogen content, quick and mobile rice leaf nitrogen content detection is realized. The machine vision technology is used, the processing technical scheme is transplanted to a mobile intelligent terminal, simple, convenient, low-cost, real-time, dynamic and quick analysis and detection on the rice leaf nitrogen content can be realized, a background comparison method is innovatively adopted, the inconvenience brought to leaf image acquisition by the previous cluttered background is solved, and interference generated when a target leaf image is extracted in the cluttered background can be effectively solved.

The invention discloses a composite modified electrode as well as a preparation method and application thereof. The gold nano-poly(3-aminophenylboronic acid) chemically modified electrode has the advantages of high sensitivity, high detection speed, no additional indicator and simple operation, and can realize rapid, accurate and quantitative detection of aluminum ions and xylitol. Detection results show that indexes such as a linear range, a detection limit and reproducibility all meet requirements, and the modified electrode can be successfully used for food detection and analysis and further used for evaluating food quality.