65results about How to "Rapid Analysis Detection" patented technology

The invention provides a method for preparing a metal ion imprinting adsorbent with an underwater selective recognition performance. The method comprises the steps of: preparation of an amino functional material: dispersing a nuclear body material into a methanol solution, slowly adding 3-propylaminoethoxy silane, uniformly stirring, and then, continuously adding a cross-linking agent and a stabilizer to form the amino functional material; and preparation of the metal ion imprinting adsorbent: dispersing the amino functional material in the methanol solution, and exposing to ultrasound to obtain a solution A; dissolving isonicotinic acid and metal template ions in deionized water to form a solution B; dripping the solution B into the solution A, and reacting to obtain a white sol-like product; and eluting the metal template ions: performing Soxhlet extraction by using an acetic acid-methanol solution, then eluting the metal template ions by using a hydrochloric acid, and drying to obtain the metal ion imprinting adsorbent. The metal ion imprinting adsorbent disclosed by the invention can be widely applied to advanced treatment of drinking water as well as enrichment recovery or rapid analysis detection of metal ions in different water bodies, such as industrial waste water, aquaculture water and seawater.

The invention relates to an electrochemical sensor capable of simultaneously detecting dopamine, ascorbic acid and uric acid. The electrochemical sensor comprises a work electrode, a reference electrode, and a counter electrode; wherein the substrate electrode of the work electrode is a glass-carbon electrode, the surface of the work electrode is coated with a composite material of graphene and mesoporous ferroferric oxide, the reference electrode is a saturated calomel electrode (SCE), and the counter electrode is a platinum wire electrode. Under an optimum condition, the electrochemical sensor can achieve the measurement of a single substance in a high concentration of coexisting substances, and can also simultaneously detect dopamine, ascorbic acid and uric acid. The electrochemical sensor not only avoids the phenomenon that the summit potentials of dopamine, ascorbic acid and uric acid overlap with each other, but also improves the sensitivity, repeatability, and stability of the conventional method.

The invention relates to a method for detecting organic pollutants by virtue of combination of solid phase micro-extraction and surface enhancement Raman. The method comprises the following steps: with a solid phase micro-extraction probe as a surface enhancement Raman active substrate, extracting for 3-10 hours in the to-be-detected environment under the stirring condition with the temperature of 20-30 DEG C, then putting the solid phase micro-extraction probe on a Raman detection platform, irradiating the solid phase micro-extraction probe by using laser to obtain Raman characteristic peaks of the to-be-detected organic pollutants, and analyzing the detected organic pollutants according to the Raman characteristic peaks. The method for detecting the organic pollutants is integrated with the advantages of the solid phase micro-extraction technology and the surface enhancement Raman technology; the organic pollutants in the environment can be quickly analyzed and detected; the sameness analysis of polyaromatic hydrocarbon can be realized; polyaromatic hydrocarbon mixtures can be detected and analyzed.

The invention belongs to the technical field of food, and more specifically relates to a color / fluorescence double signal visible rapid nitrite detection method, and applications thereof. The color / fluorescence double signal visible rapid nitrite detection method comprises following steps: firstly, a carbon dot-neutral red composite system is prepared, wherein carbon dot synthesis and constructionof the carbon dot-neutral red composite system are carried out; a common filter paper is immersed in the carbon dot-neutral red composite system so as to obtain a carbon dot-neutral red composite system detection test paper; a sodium nitrite standard solution is prepared; a color standard colourimetric card and a fluorescence standard colourimetric card of nitrites are prepared; a software is adopted to extract the RGB values of each test paper, a matrix is constructed, and a multivariate linear regression model is constructed; and the content of nitrites in food to be detected is detected. The carbon dot-neutral red composite system possess color / fluorescence double response capacity on nitrites; detection operation steps are simple; the visible results are clear, visual, and accurate; the detection limit is lower; and it is promising for the color / fluorescence double signal visible rapid nitrite detection method to be used in large-scale food nitrite content detection.

The invention discloses a method for determining glucose based on a paper-based electrochemical device. The method comprises the following steps that 1, filter paper serves as a detection platform of a paper-based substrate, an electrode is manually drawn with a pencil, and graphite serves as an electrode material, wherein the detection platform comprises two areas, namely a detection area and an enzyme area, each area is provided with a round hydrophilic area, and the part except the hydrophilic area is a hydrophobic area; 2, according to the principle of glucose oxidase oxidizing reaction, ferrocenecarboxylic acid serves as an electronic mediator, glucose is determined by adopting an amperometric method, and the detection platform can sensitively determine the glucose in the presence of interferent; a sample to be detected is added to the detection platform, and the actual blood sugar concentration of the sample can be detected. The paper-based electrochemical system adopted in the method is the novel electrochemical device, the electrochemical device is simple in manufacture and low in cost and can be used in lots of application fields including portable and disposable analytical equipment for clinical diagnosis, food quality control, environment monitoring and the like.

The invention provides a chiral chromatographic separation and analysis method of dihydromyricetin enantiomer. The method comprises the following steps: dissolving a dihydromyricetin sample into the mobile phase, taking polysaccharide derivatives as the chiral stationary phase, and carrying out liquid chromatogram (LC) separation and analysis; wherein the mobile phase is a mixed solution of n-hexane and lower alcohols, and the polysaccharide derivative chiral stationary phase is one of stationary phases, which are individually covered by tri(3,5-dimethylphenylcarbamyl) straight-chain starch, tri((S)-(alpha)- phenylethylcarbamyl) straight-chain starch, tri(3,5- dimethylphenylcarbamyl)cellulose and tri(4- methylphenylcarbamyl)cellulose. According to the provided chiral separation and analysis method, the maximal separation degree of dihydromyricetin enantiomer is 1.95, and the maximal selection factor is 1.36.

The invention discloses an application of an electrochemiluminescence biosensor based on cytosine (C)-containing cyclic DNA sequences where in-situ reduced Ag nano-clusters are enriched as signal probes and adopting dual amplification strategy to detection of target thrombin. According to the technical scheme, DNA cleavage enzyme with hairpin DNA recognition capability and catalysis effect is designed, when target thrombin exists, hairpin DNA is opened, and substrate DNA is cleaved under the action of Zn<2+>; a large quantity of cytosine (C)-containing cyclic DNA sequences are aggregated through HCR (hybridization chain reaction), in-situ reduction of AgNO3 is performed on the electrode surface by NaBH4, a large quantity of Ag nano-clusters are formed, and the electrochemiluminescence biosensor with dual amplification effect is prepared. The sensor is subjected to luminescence detection, and a linear relation is formed between light-emitting signals and concentration of a to-be-testedsample. The Ag nano-cluster signal probes and the DNA dual amplification technology are combined for rapid and high-sensitivity detection of thrombin, and the electrochemiluminescence biosensor has great application potential in early clinical analysis and detection.

The invention discloses a microflow control detection chip for detecting cell surface sign, which comprises plate substrate, where the substrate is carved with microflow channel, the side carved with microflow channel is sealed with glass piece, and a sealed microflow channel for holding to-be-detected cell sample is formed between the glass piece and microflow channel. And it pumps unreacted bioprobe into the microflow channel and then pumps the to-be-deteced cell into the microflow channel and uses buffer liquor to wash the cell and analyzes reaction in the microflow channel and obtains the detection result without fluorescent labeling and purifying for to-be-detected protein.

The invention relates to an electrochemical sensor capable of simultaneously detecting dopamine, ascorbic acid and uric acid. The electrochemical sensor comprises a work electrode, a reference electrode, and a counter electrode; wherein the substrate electrode of the work electrode is a glass-carbon electrode, the surface of the work electrode is coated with a composite material of graphene and mesoporous ferroferric oxide, the reference electrode is a saturated calomel electrode (SCE), and the counter electrode is a platinum wire electrode. Under an optimum condition, the electrochemical sensor can achieve the measurement of a single substance in a high concentration of coexisting substances, and can also simultaneously detect dopamine, ascorbic acid and uric acid. The electrochemical sensor not only avoids the phenomenon that the summit potentials of dopamine, ascorbic acid and uric acid overlap with each other, but also improves the sensitivity, repeatability, and stability of the conventional method.

The invention discloses a photoelectric double-pulse laser induced breakdown spectrograph, comprising a pulse laser, a focusing lens, a movable platform, a discharge electrode, an optical diode, a pulse time-delay controller, a high-voltage pulse power supply, an optical collection system of optical radiation, a monochromator or a spectrograph, a photoelectric conversion element, a data collection unit and an electronic computer. The invention also discloses a spectral analysis method of the photoelectric double-pulse laser induced breakdown spectrograph. The photoelectric double-pulse laser induced breakdown spectrograph can greatly enhance the spectral analysis sensitivity.

The invention relates to a security management and control system and a security management and control method for online behaviors combining artificial intelligence and big data. The method comprisesthe following steps: (1) establishing a back-end database, comprising user biometrics and behavior rule attribute data; (2) acquiring network user identities and behavior data information; (3) authenticating the user identities; (4) identifying the user's behaviors; (5) auditing operations of the user's behaviors; and (6) performing risk prevention and control on the security of the user's behaviors. The security management and control system and a security management and control method for online behaviors combining artificial intelligence and big data can make up for the limitation of the traditional technology on the processing of massive behavior data, accurately predict the behavior categories of the network users, quickly analyze and detect the behaviors of the online users, comprehensively and stereoscopically monitor the behaviors of the users in the network space, effectively implement the dynamic awareness of online behavioral security, realize dynamic quantitative assessment of security risks, and achieve hierarchical warning.

The invention belongs to the technical field of a biosensor and discloses a preparation method of the electrochemiluminescence biosensor for detecting mercury ions. Carbon nitride quantum dots are taken as a new-type co-reacant, so an electrochemiluminescence signal of a luminophor [Ru(dcbpy)<3><2+>] can be enhanced. In order to realize specific detection for the mercury ions, DNA1 and DNA2 rich in T bases are imported, and the DNA1 and the DNA2 are connected with the luminophor and the co-reacant. Pairing bonding can be carried out on the DNA1 and the DNA2 through utilization of the mercury ions. Distance between the luminophor and the co-reacant is closer, so the electrochemiluminescence signal is further enhanced. According to the constructed electrochemiluminescence biosensor, a detection limit for the mercury ions is 3.3x10 <-15>mol.L<-1>. The electrochemiluminescence biosensor provided by the invention is low in cost, high in sensitivity and good in selectivity and is applicableto practical application detection of mercury ion content in surface water.

The invention provides an OTA (Ochratoxin A) chemiluminescence detection method based on a label-free aptasensor. According to the technical scheme, the OTA chemiluminescence detection method comprises: fixing an aptamer capturing probe on the surface of a magnetic microsphere; combining the capturing probe and OTA with a competition relationship of an OTA aptamer respectively; obtaining a quantitative relationship between the quantity of magnetic microsphere surface detection probes and the concentration of OTA to be detected based on the fact that the specific combining capability of the aptamer is stronger than the binding force of complementary strands; finally, carrying out chemiluminescence detection on the aptasensor. A chemiluminescence detection mechanism of the OTA chemiluminescence detection method is realized based on a principle that a guanine base (G) on an OTA aptamer strand and a chemiluminescence reagent phenylglyoxal (PG) are subjected to an instantaneous derivative reaction to generate chemiluminescence, so that a labeling link of other chemiluminescence markers is omitted; operation steps are simplified on the basis of guanrateeing the sensitivity and the detection time is shortened; a chemiluminescence anslysis method has the advantages of wide linear range, simple equipment and easiness of operation, so that full-automatic detection is hopeful to realize.

The invention relates to a mass spectrum ion source device. The mass spectrum ion source device comprises a point electrode, an insulating medium, a power supply, an instrument interface and a gas conduct; the insulating medium is located between the point electrode and the instrument interface; a tip part of the point electrode is contacted with the insulating medium, and the other end of the point electrode is connected with the power supply; the instrument interface is grounded and connected with the other end of the power supply; a strong electric field is formed between the point electrode and the instrument interface; discharge gases are filled between the insulating medium and the instrument interface through the gas conduit, and a plasma beam is formed under the action of the strong electric field; and the plasma beam interacts with an object to be measured on the surface of the insulating medium to ionize the object to be measured, so as to realize analysis and detection. The mass spectrum ion source device provided by the invention has the advantages of being simple in structure, low in cost, no solvent and high in flux and so on, besides, a linear electric field formed by the point electrode and the instrument interface improves the transmission efficiency of ions. Moreover, the mass spectrum ion source device can also be used for mass spectrometry imaging analysis.

The invention relates to a wu-he dipsacus asper ISSR-PCR molecule marking method. The method is characterized by comprising the following steps: 1, extracting the genome of the wu-he dipsacus asper, 2, conducting 1% agarose electrophoresis for 20 minutes, testing the integrity of the genome, at the same time using the DNA bar code ITS2 primer to conduct the PCR amplification of the extracted wu-he dipsacus asper DNA, verifying the purity of the extracted DNA, 3, based on the initial screened primer conducting the ISSR-PCR. Compared with the prior art, the reaction system created by the method has a simple optimization method. The amplified bands is both clear and stable with good polymorphism. The method compensates the shortcomings in the study of the genetic diversity of the wu-he dipsacus asper. The method can be used in the analysis of the genetic relationship and the verification of authenticity of the teasle, thus having good application value.

The invention discloses a preparation method for a porous polymer microsphere anion filling material. The preparation method comprises the following steps: a chloromethyl group is introduced at first, and then ethene diamine is introduced onto the surfaces of microspheres by using a surface grafting method. According to the invention, monodisperse polystyrene / divinyl benzene microspheres are used as a substrate which reacts with chloromethyl ether for introduction of a chloromethyl functional group and then reacts with ethene diamine via the surface grafting method so as to form the anion filling material. The anion filling material obtained in the invention has the characteristics of controllable microsphere size and controllable varieties of surface function groups; and the preparation method has the characteristics that the method is simple and easily practicable in process, low in cost for raw materials, easy to operate and easy for industrial application.

The invention relates to an atmospheric pressure photoionization mass spectrum device for analyzing solid fuel pyrolysis on line. The device comprises a pyrolyzing furnace, an auxiliary volatile solvent introduction mechanism, an atmospheric pressure photoionization source and a mass spectrometer. With the increasingly exhausted fossil fuel, a process of preparing various hydrocarbon fuels and petrochemical materials through solid fuel pyrolysis is gradually concerned by people. According to the invention, the species distribution and generation mechanism of solid fuel pyrolysis products such as solid wastes (such as polypropylene), biomass, coal and oil shale can be accurately and rapidly analyzed on line in real time, people can be helped to well master the internal rules of pyrolysis, effective resource utilization is comprehensively realized, and the device has great significance on sustainable development of China.

The invention discloses a trace substance detector with two fluorescence emission surfaces, and a detection method, and relates to an instrument used for detecting toxic and hazardous trace substances contained in environments, food, health care products, traditional Chinese medicines, cosmetics and the like in the technical field of spectrographic detection. The detector comprises an excitation unit, a first detection unit and a second detection unit, wherein the first detection unit is parallel to the second detection unit; the excitation unit is perpendicular to the first detection unit. Compared with a dual-waveband fluorescence intensity ratio detection system with a single fluorescence emission surface, the detector is not provided with a dichroic mirror or a fluorescence condensing lens on the basis of keeping the detection stability and an accuracy rate, so that a fluorescence loss is low; an optical path is short; the sensitivity is higher; a detection limit is lower. Under the equivalent condition of copper ion detection, by comparison of an optical system with two fluorescence emission surfaces and an optical system with a single fluorescence emission surface, the fluorescence detection strength is improved by 1.2 times and the detection limit is reduced from 0.03 mg / kg to 0.015 mg / kg. The detector is smaller in size and simple to operate, and can be used for on-site real-time quantitative rapid analysis and detection.

The invention discloses preparation and application of a 3-nitrotyrosine molecularly imprinted electrochemical transducer. The preparation comprises the steps: firstly dispensing multiwall carbon coupled graphene oxide nanoribbon on a glassy carbon electrode to obtain a functional nano material electrode; polymerizing molecularly imprinted polymer and depositing doped nanogold on the surface of the electrode in doped nanogold-molecularly imprinted polymer solution to form a layer of molecularly imprinted membrane; removing 3-nitrotyrosine template molecules to obtain a functional nano material molecularly imprinted electrochemical transducer. The transducer disclosed by the invention has good selectivity and high flexibility on a biomarker of 3-nitrotyrosine, is simple to manufacture, has stable performance, and can be used repeatedly; the preparation cost is low, a sample is simple to pretreat, and the detection is quick; equipment is portable and suitable for site detection.

The invention discloses a method for analyzing and detecting trace metal ion impurities in dimethyl diallyl ammonium chloride (DMDAAC) monomers. According to the method, an organic solvent chelating extraction-flame atomic absorption spectroscopy method is adopted to measure the contents of multiple trace metal ions in DMDAAC monomers of a DMDAAC monomer solution with a high salt content, and avoids the difficult operation and result errors caused by direct injection of a sample with a high salt content. By utilizing the synergistic effect of a complexing agent, three metal ions (Fe, Cu, and Ni) can be complexed, extracted, separated, analyzed, and tested at the same time. The provided method has the advantages that the operation is simple and convenient, the analysis precision is high, and the contents of several trace metal ion impurities in DMDAAC monomers can be accurately, rapidly, and reliably analyzed and detected. The provide method has an important scientific meaning and industrial practical value for industrial manufacturing of high purity DMDAAC monomers, establishment of national / international standards of DMDAAC monomers, and research on the activity of DMDAAC monomers in polymerization reactions.

The invention discloses a fluorescent biosensor based on an enzyme-catalyzed target cyclic amplification technology combined with carboxyl functionalized carbon quantum dots as well as a preparation method and application of the fluorescent biosensor. A technical solution adopted in the invention is that a tumor-associated gene miRNA34c is used as a fuel molecule to design a multi-wheel driven DNAnanomachine comprising an unmarked stem-loop structure 1 (HP1), a stem-loop structure 2 (HP2) and a driving primer (DP) for cyclic amplification of a trace target. In addition, the synthesized carboxyl carbon quantum dots (cCQD) with excellent biocompatibility firstly is bound to a fluorescent probe to quench a fluorophore; an S1 cycle product in a target stimulating cyclic amplification processis added and placed for 20 min at room temperature to recover fluorescence; then, a fluorescence intensity of the mixture is measured so as to realize sensitive detection of the target miRNA34c. The research idea provides a new strategy for the sensitive detection of miRNA34c and is expected to be used in early diagnosis of tumors.

The invention discloses a method for rapidly distinguishing the variety of a metal material. The method comprises an X-ray diffractometer, the X-ray is classically scattered when encountering an electron tightly bonded in a crystal, the crystal comprises a large quantity of atoms, each atom further comprises a plurality of electrons, the classic scattering rays produced by the electrons are mutually interfered, certain direction is strengthened, another direction is weakened, the overall interferential result of the electron engineering rays is called diffraction, the diffraction is used for measuring the crystal structure, analyzing a phase quantitatively and qualitatively, measuring the lattice parameter accurately, and also measuring the internal stress, the texture and the grain size of the material, the metal material is conveniently rapidly analyzed and detected, the analysis and detection result is accurate, and the method can be applied to the practical production conveniently.

The invention discloses a preparation method of a bongkrekic acid high-selectivity enrichment solid-phase micro-extraction probe. The preparation method comprises the following steps of: providing a solid substrate; carrying out surface modification on the solid substrate to enable the surface of the solid substrate to be rich in methacryloyloxy so as to obtain a surface modified solid substrate; and carrying out a molecular imprinting polymerization reaction on the surface modified solid substrate through a molecular imprinting technology by taking methacryloyloxyethyl trimethyl ammonium chloride and octadecyl methacrylate as functional monomers and vitamin A acid and (R)-12-hydroxy-cis-9-octadecenoic acid as template molecules to enable a molecularly imprinted coating with high selective adsorption capacity on bongkrekic acid to be attached to the surface of the solid substrate. The solid-phase micro-extraction probe disclosed by the invention can be used for efficiently extracting and enriching bongkrekic acid in complex food, the enrichment coefficient can reach thousands of times, and the detection limit can reach the level of [mu]g / L. The probe is simple to manufacture, low in price, disposable, capable of effectively preventing cross contamination and memory effect, and especially suitable for rapid analysis and detection of food safety.

The invention discloses a mobile rice leaf nitrogen content detecting and grading method based on machine vision. By using analysis on the correlation between optical indexes provided by a digital image and a rice leaf nitrogen content, quick and mobile rice leaf nitrogen content detection is realized. The machine vision technology is used, the processing technical scheme is transplanted to a mobile intelligent terminal, simple, convenient, low-cost, real-time, dynamic and quick analysis and detection on the rice leaf nitrogen content can be realized, a background comparison method is innovatively adopted, the inconvenience brought to leaf image acquisition by the previous cluttered background is solved, and interference generated when a target leaf image is extracted in the cluttered background can be effectively solved.

The invention provides a method for preparing a metal ion imprinting adsorbent with an underwater selective recognition performance. The method comprises the steps of: preparation of an amino functional material: dispersing a nuclear body material into a methanol solution, slowly adding 3-propylaminoethoxy silane, uniformly stirring, and then, continuously adding a cross-linking agent and a stabilizer to form the amino functional material; and preparation of the metal ion imprinting adsorbent: dispersing the amino functional material in the methanol solution, and exposing to ultrasound to obtain a solution A; dissolving isonicotinic acid and metal template ions in deionized water to form a solution B; dripping the solution B into the solution A, and reacting to obtain a white sol-like product; and eluting the metal template ions: performing Soxhlet extraction by using an acetic acid-methanol solution, then eluting the metal template ions by using a hydrochloric acid, and drying to obtain the metal ion imprinting adsorbent. The metal ion imprinting adsorbent disclosed by the invention can be widely applied to advanced treatment of drinking water as well as enrichment recovery or rapid analysis detection of metal ions in different water bodies, such as industrial waste water, aquaculture water and seawater.

The invention discloses a composite modified electrode as well as a preparation method and application thereof. The gold nano-poly(3-aminophenylboronic acid) chemically modified electrode has the advantages of high sensitivity, high detection speed, no additional indicator and simple operation, and can realize rapid, accurate and quantitative detection of aluminum ions and xylitol. Detection results show that indexes such as a linear range, a detection limit and reproducibility all meet requirements, and the modified electrode can be successfully used for food detection and analysis and further used for evaluating food quality.

The invention provides a chiral chromatographic separation and analysis method of dihydromyricetin enantiomer. The method comprises the following steps: dissolving a dihydromyricetin sample into the mobile phase, taking polysaccharide derivatives as the chiral stationary phase, and carrying out liquid chromatogram (LC) separation and analysis; wherein the mobile phase is a mixed solution of n-hexane and lower alcohols, and the polysaccharide derivative chiral stationary phase is one of stationary phases, which are individually covered by tri(3,5-dimethylphenylcarbamyl) straight-chain starch, tri((S)-(alpha)- phenylethylcarbamyl) straight-chain starch, tri(3,5- dimethylphenylcarbamyl)cellulose and tri(4- methylphenylcarbamyl)cellulose. According to the provided chiral separation and analysis method, the maximal separation degree of dihydromyricetin enantiomer is 1.95, and the maximal selection factor is 1.36.

The invention belongs to the technical field of ocean microparticle detection, and discloses a cylindrical capacitance detecting device for detecting ocean microplastic. The device comprises a microfluidic detecting chip, a cylindrical capacitance detecting unit embedded in the microfluidic detecting chip, and an exciting unit; the exciting unit is connected with the cylindrical capacitance detecting unit through an insulation lead; the cylindrical capacitance detecting unit comprises two cylindrical capacitance polar plates with the same structures; the two cylindrical capacitance polar plates are arranged in an aligned mode; and a microchannel in the microfluidic detecting chip penetrates between the two cylindrical capacitance polar plates, and is tightly bonded on the microchannel. The cylindrical capacitance polar plates are excited by high-frequency alternating current through the exciting unit; and when microplastic particles in seawater pass through the cylindrical capacitance polar plates, as the relative dielectric constant of the water is higher than the relative dielectric constant of the microplastic, negative capacitance signal pulse is generated, the sizes and the shapes of the microplastic are judged according to the amplitude magnitude and the frequency of the generated signal pulse, and the problems of low detecting speed, low detecting precision and complex detecting equipment in the prior art are solved.