Preparation method of electrochemiluminescence biosensor for detecting mercury ions

A luminescent biological and electrochemical technology, applied in the field of biosensors, can solve the problems of large-scale equipment and high operation requirements, and achieve the effect of simple preparation method, simplified complexity, and sensitive analysis and detection

Active Publication Date: 2019-07-16
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While these methods are sensitive and precise, they are operationally demanding and require large equipment

Method used

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  • Preparation method of electrochemiluminescence biosensor for detecting mercury ions
  • Preparation method of electrochemiluminescence biosensor for detecting mercury ions
  • Preparation method of electrochemiluminescence biosensor for detecting mercury ions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Concrete preparation method of the present invention is as follows:

[0037] (1)Ru(dcbpy) 3 2+ - Preparation of DNA1 composites:

[0038]Dissolve 4 mg of tris(4,4-dicarboxybipyridyl) ruthenium chloride in 8 mL of buffer solution (pH=7), add 2 mL of a mixed solution containing 160 mg of EDC and 40 mg of NHS into the above solution and stir at room temperature for 2 h, then add 1.6 mL of 0.5 -3μM DNA1 continued to stir for 6-12h, this time the stirring environment was 25°C, 100rpm, then added 800μL 0.5-5M sodium acetate solution and 16mL ethanol to carry out precipitation reaction at -30-1°C for 5-12h, and centrifuged at 12000rpm for 30min to obtain The precipitate was washed twice with 70% ethanol, and finally, after drying at room temperature, it was dissolved in 1 mL of buffer solution (pH=7) and placed in a refrigerator at 4°C for later use.

[0039] (2)g-C 3 N 4 Preparation of QDs-DNA2 composites:

[0040] 400μL mixture containing 40mM EDC and 10mM NHS added to...

Embodiment 2

[0050] Utilize the present invention to detect the liquid to be tested, the steps are as follows:

[0051] (1)Ru(dcbpy) 3 2+ - Preparation of DNA1 composites:

[0052] Dissolve 4 mg of tris(4,4-dicarboxybipyridyl) ruthenium chloride in 8 mL of buffer solution (pH=7), add 2 mL of a mixed solution containing 160 mg of EDC and 40 mg of NHS into the above solution and stir at room temperature for 2 h, then add 1.6 mL of 0.5 -3μM DNA1 continued to stir for 6-12h, this time the stirring environment was 25°C, 100rpm, then added 800μL 0.5-5M sodium acetate solution and 16mL ethanol to carry out precipitation reaction at -30-1°C for 5-12h, and centrifuged at 12000rpm for 30min to obtain The precipitate was washed twice with 70% ethanol, and finally, after drying at room temperature, it was dissolved in 1 mL of buffer solution (pH=7) and placed in a refrigerator at 4°C for later use.

[0053] (2)g-C 3 N 4 Preparation of QDs-DNA2 composites:

[0054] 400μL mixture containing 40mM E...

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Abstract

The invention belongs to the technical field of a biosensor and discloses a preparation method of the electrochemiluminescence biosensor for detecting mercury ions. Carbon nitride quantum dots are taken as a new-type co-reacant, so an electrochemiluminescence signal of a luminophor [Ru(dcbpy)<3><2+>] can be enhanced. In order to realize specific detection for the mercury ions, DNA1 and DNA2 rich in T bases are imported, and the DNA1 and the DNA2 are connected with the luminophor and the co-reacant. Pairing bonding can be carried out on the DNA1 and the DNA2 through utilization of the mercury ions. Distance between the luminophor and the co-reacant is closer, so the electrochemiluminescence signal is further enhanced. According to the constructed electrochemiluminescence biosensor, a detection limit for the mercury ions is 3.3x10 <-15>mol.L<-1>. The electrochemiluminescence biosensor provided by the invention is low in cost, high in sensitivity and good in selectivity and is applicableto practical application detection of mercury ion content in surface water.

Description

technical field [0001] The invention belongs to the technical field of biosensors, in particular to a method for preparing an electrochemiluminescent biosensor for detecting mercury ions. Background technique [0002] Mercury ions (Hg 2+ ) wastes discharged into the environment will cause harm to soil, water and crops. For example, it causes the disorder of the physiological function of the crops and the imbalance of nutrition, which eventually causes the crops to wither or even die, resulting in the reduction of crop yield and quality. Hg 2+ After entering the human body through the food chain, it will be enriched in the body, causing nervous system disorders, and even poisoning death. Therefore for Hg in water 2+ It is necessary to detect the content of water, which can provide a theoretical basis for water quality assessment and subsequent treatment [0003] for Hg 2+ The detection of Hg has always been a matter of great concern to people, and the commonly used Hg ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N21/76
CPCG01N21/76G01N27/305G01N27/308
Inventor 由天艳张家一李丽波罗莉君刘晓红
Owner JIANGSU UNIV
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