Micro fluid control chip for investigating cell surfact marker

Abstract

The invention discloses a microflow control detection chip for detecting cell surface sign, which comprises plate substrate, where the substrate is carved with microflow channel, the side carved with microflow channel is sealed with glass piece, and a sealed microflow channel for holding to-be-detected cell sample is formed between the glass piece and microflow channel. And it pumps unreacted bioprobe into the microflow channel and then pumps the to-be-deteced cell into the microflow channel and uses buffer liquor to wash the cell and analyzes reaction in the microflow channel and obtains the detection result without fluorescent labeling and purifying for to-be-detected protein.

Description

technical field [0001] The invention relates to a microfluidic detection chip for detecting cell surface markers. Background technique [0002] Cell surface markers are a class of physiologically important protein molecules that are expressed on the cell surface and can usually be used to characterize specific cell types. At present, such surface marker molecules have been widely used in basic research and clinical practice, such as the determination of cellular immune prototypes and the diagnosis of diseases. The current main tool for detecting surface markers is the flow cytometer, whose working principle is to mix and react fluorescently labeled antibodies with the cells to be detected. Strict quantification of markers can be achieved through the detection of fluorescent information. Therefore, flow cytometry Cytometers are generally considered to be one of the most effective tools for detecting surface markers. However, the flow cytometer is a low-throughput analysis s...

AI Technical Summary

Problems solved by technology

Protein microarray chips can perform fast, high-throughput analysis of the target object, and the analysis cost is very low, but the application of protein microarray chips to detect surface markers usually requires a complicated protein purification process, and the array chips themselves are susceptible to environmental degradation. influence (such as environmental humidity, cleanliness and other factors); at the same time, because the static detection and analysis of the array chip is difficult to realize the automation of the whole process, some key steps in the analysis process are susceptible to the influence of manual operations (such as the application of buffer solution to remove unreacted substances or reagents on the chip, etc.), thus affecting the accuracy and reliability of the detection results

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