Electrochemical sensor capable of simultaneously detecting dopamine, ascorbic acid and uric acid

An ascorbic acid and electrochemical technology, applied in the direction of material electrochemical variables, can solve the problems of selectivity and unsatisfactory detection limit, and achieve the effect of excellent accuracy, uniform film and low detection limit

Active Publication Date: 2014-02-26
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its selectivity and detection limit are not very satisfactory

Method used

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  • Electrochemical sensor capable of simultaneously detecting dopamine, ascorbic acid and uric acid
  • Electrochemical sensor capable of simultaneously detecting dopamine, ascorbic acid and uric acid
  • Electrochemical sensor capable of simultaneously detecting dopamine, ascorbic acid and uric acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042](1) Synthesis of composites of graphene and mesoporous ferric oxide

[0043] Synthesis of graphene oxide: Mix 0.3 g of graphite powder and 1.8 g of potassium permanganate, put them into a three-neck flask with a magnet, add 40 mL of concentrated sulfuric acid and phosphoric acid mixture with a volume ratio of 9:1 to the above three-neck bottle. Put the three-neck flask into an oil bath, heat it to 50 °C, and react for 12 hours. After the reaction is over, pour the sample onto about 40 mL of ice, and at the same time, put the magnet on the poured ice-water mixture, and slowly magnetically stir , add 0.3 mL of 30% hydrogen peroxide, the ice melts gradually, and magnetically stir for 0.5 h. After the reaction, the obtained mixture was centrifuged at 8000 R for 0.5 h, then washed three times with 20 mL of water, 20 mL of 30% hydrochloric acid, and 20 mL of ethanol, discarded the supernatant, and finally washed with 20 mL of Centrifuge and wash with mL ether, discard the su...

Embodiment 2

[0056] Example 2 The detection of uric acid in the presence of ascorbic acid and dopamine

[0057] According to the steps of Example 1, an electrochemical sensor was constructed for the detection of uric acid. The concentration of DA and AA is fixed, the concentration of UA is changed, and the electrode modified by graphene and mesoporous ferric oxide composites is shown in the differential pulse voltammogram of the three substances. figure 2 shown. Depend on figure 2 It can be seen that the oxidation peaks of the three substances are clearly separated and the peak shapes are better. At 1.0~850μmol·L -1 Within the concentration range, the maximum peak current value of UA is proportional to the square root of its concentration, and the linear correlation coefficient r =0.993, the detection limit is 0.056μmol L -1 .

Embodiment 3

[0058] The detection of ascorbic acid when embodiment 3 uric acid, dopamine exist

[0059] According to the steps of Example 1, an electrochemical sensor was constructed for the detection of ascorbic acid. Fix the concentration of DA and UA, change the concentration of AA, and draw the differential pulse voltammogram of the modified electrode to the three substances. It can be obtained from the figure that in the range of 5.0~1600μmol·L -1 The maximum peak current value of AA within the concentration range is proportional to the square root of its concentration, and the linear correlation coefficient r =0.984, the detection limit is 0.074μmol L -1 .

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Abstract

The invention relates to an electrochemical sensor capable of simultaneously detecting dopamine, ascorbic acid and uric acid. The electrochemical sensor comprises a work electrode, a reference electrode, and a counter electrode; wherein the substrate electrode of the work electrode is a glass-carbon electrode, the surface of the work electrode is coated with a composite material of graphene and mesoporous ferroferric oxide, the reference electrode is a saturated calomel electrode (SCE), and the counter electrode is a platinum wire electrode. Under an optimum condition, the electrochemical sensor can achieve the measurement of a single substance in a high concentration of coexisting substances, and can also simultaneously detect dopamine, ascorbic acid and uric acid. The electrochemical sensor not only avoids the phenomenon that the summit potentials of dopamine, ascorbic acid and uric acid overlap with each other, but also improves the sensitivity, repeatability, and stability of the conventional method.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and relates to an electrochemical sensor. More specifically, the invention relates to an electrochemical sensor based on graphene and mesoporous ferric oxide composite material, which is used for dopamine, ascorbic acid and Simultaneous detection of uric acid. Background technique [0002] Dopamine (Dopamine Acid, DA) is a neurotransmitter used to help cells transmit impulses. This brain endocrine is mainly responsible for the brain's lust and feeling, responsible for the information transmission of happiness and excitement, and is also related to addiction. In addition, dysregulation of dopamine in the body is an important cause of neuromuscular disorders, Parkinson's disease, heart disease, and various mental illnesses. [0003] Ascorbic acid (AA) is a water-soluble vitamin necessary for human life activities. It plays a regulatory role in redox metabolic reactions, and the lack...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/26
Inventor 吴丹魏琴李玉阳杜斌张勇
Owner UNIV OF JINAN
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