Fluorescent biosensor based on cyclic amplification technology and carboxyl carbon quantum dots as well as preparation method and application of fluorescent biosensor

A technology of biosensors and carbon quantum dots, applied in the field of biosensors, can solve the problems of complex and strict preparation of nanomaterials, difficulty in ensuring the biocompatibility of nanomaterials, etc., and achieve excellent accuracy, great application potential, and rapid analysis and detection Effect
CN109444098AInactive Publication Date: 2019-03-08QINGDAO UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
QINGDAO UNIV OF SCI & TECH
Publication Date
2019-03-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a fluorescent biosensor based on an enzyme-catalyzed target cyclic amplification technology combined with carboxyl functionalized carbon quantum dots as well as a preparation method and application of the fluorescent biosensor. A technical solution adopted in the invention is that a tumor-associated gene miRNA34c is used as a fuel molecule to design a multi-wheel driven DNAnanomachine comprising an unmarked stem-loop structure 1 (HP1), a stem-loop structure 2 (HP2) and a driving primer (DP) for cyclic amplification of a trace target. In addition, the synthesized carboxyl carbon quantum dots (cCQD) with excellent biocompatibility firstly is bound to a fluorescent probe to quench a fluorophore; an S1 cycle product in a target stimulating cyclic amplification processis added and placed for 20 min at room temperature to recover fluorescence; then, a fluorescence intensity of the mixture is measured so as to realize sensitive detection of the target miRNA34c. The research idea provides a new strategy for the sensitive detection of miRNA34c and is expected to be used in early diagnosis of tumors.
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Description

Technical field:

[0001] The invention relates to a biosensor based on the enzymatic target cyclic amplification technology combined with carboxyl-functionalized carbon quantum dot fluorescent probes; the invention also relates to a preparation method of the biosensor and its analytical application for detecting miRNA. Background technique:

[0002] MicroRNAs (miRNAs) are a class of endogenous non-coding RNA molecules (20-23nt) that are targeted for degradation by translational inhibition or by forming RNA-induced silencing complex (RISC) with target messenger RNAs. Physiological processes such as proliferation, development, metabolism, immune response, tumor, and viral infection in biology have a crucial impact [Lee I, Ajay S S, Chen H, et al. Nucleic Acids Research, 2008, 36(5): e27.]. Due to the low abundance and easy degradation of miRNAs, direct detection can only be detected at trace amounts. Therefore, there is an urgent need to develop sensitive, highly selective, co...

Claims

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