Preparation and application of 3-nitrotyrosine molecularly imprinted electrochemical transducer

A nitrotyrosine and molecular imprinting technology, applied in the field of chemical analysis, can solve the problems of slow electron transfer speed and response, affecting the application of molecular imprinting technology, difficult to control the thickness of the imprinting film, etc., achieving convenient on-site detection and fast detection speed. , Sensitive and reliable detection

Active Publication Date: 2017-08-22
康正检测服务股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the thickness of the imprinted film prepared by the traditional imprinting method is difficult to control, and the high cross-linking degree makes the electron transfer speed and response slow, the detection limit is high, and the regeneration and reversibility are poor, which affects the application of molecular imprinting technology in electrochemical sensors.

Method used

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  • Preparation and application of 3-nitrotyrosine molecularly imprinted electrochemical transducer
  • Preparation and application of 3-nitrotyrosine molecularly imprinted electrochemical transducer
  • Preparation and application of 3-nitrotyrosine molecularly imprinted electrochemical transducer

Examples

Experimental program
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Effect test

Embodiment 1

[0040] The preparation of embodiment 1 electrode

[0041] 1) Pretreatment of the glassy carbon electrode: the glassy carbon electrode is sequentially treated with 0.5 μm and 0.05 μm Al 2 o 3 The powder is polished on suede, rinsed with ultrapure water and ultrasonically cleaned in 50% nitric acid solution, ethanol and water for 5 minutes, and then the electrode is scanned in 5mmol / L potassium ferricyanide until a reversible cycle is obtained. Volt-ampere peak;

[0042] 2) Preparation of multi-walled carbon-coupled graphene oxide nanobelt nanomaterials: 120mg MWCNT dispersed in 40mLH 2 SO 4 / H 3 PO 4 (9:1 molar ratio) solution, stirred at room temperature for 1h. Then slowly add 600mg KMnO to the above solution 4 Then, under the condition of 65°C, heat in a water bath for 2h. Finally, 400 mL of ice water (containing 5 mL of 30% H 2 o 2 ) to terminate the reaction. Filtrate with a polytetrafluoroethylene membrane, wash with water, wash with ethanol, and finally dry in...

Embodiment 2

[0046] The drawing of embodiment 2 working curve and the mensuration of limit of detection

[0047] The responsivity of 3-nitrotyrosine molecularly imprinted electrochemical sensor was tested by square wave stripping voltammetry, and the linear range and detection limit were determined. The 3-nitrotyrosine molecularly imprinted electrochemical sensor was immersed in different 3-nitrotyrosine standards, and then square-wave stripping voltammetry was performed. With Ag / AgCl as the reference electrode, Pt electrode as the counter electrode, molecular imprinted electrochemical sensor as the working electrode, connected to the electrochemical workstation, in different concentrations of 3-nitrotyrosine, the scanning potential is 1.02V, Detect the relationship between the change of the electrical signal before and after the elution of the electrochemical sensor and the adsorption of template molecules and the concentration of 3-nitrotyrosine, and draw a working curve, such as figure...

Embodiment 3

[0050] Embodiment 3 Regeneration, reproducibility and interference experiment of modified electrode

[0051] Five 3-nitrotyrosine molecularly imprinted electrochemical sensors were prepared under the conditions described in Example 1, using the same 3-nitrotyrosine molecularly imprinted electrochemical sensor pair 5.0×10 -5 mol / L 3-nitrotyrosine for 5 consecutive measurements, after each measurement, the electrode surface must be cleaned with deionized water. The relative standard deviation of the calculated current response value is 3.2% (n=5), indicating that the molecularly imprinted electrode has good reproducibility. The combination of the template molecule and the "hole" on the imprinted membrane is a reversible process, and the 3-nitrotyrosine molecularly imprinted electrochemical sensor can be used repeatedly. In addition, using the modified electrode stored for three months in the dark to measure the same concentration of 3-nitrotyrosine, the oxidation peak current r...

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Abstract

The invention discloses preparation and application of a 3-nitrotyrosine molecularly imprinted electrochemical transducer. The preparation comprises the steps: firstly dispensing multiwall carbon coupled graphene oxide nanoribbon on a glassy carbon electrode to obtain a functional nano material electrode; polymerizing molecularly imprinted polymer and depositing doped nanogold on the surface of the electrode in doped nanogold-molecularly imprinted polymer solution to form a layer of molecularly imprinted membrane; removing 3-nitrotyrosine template molecules to obtain a functional nano material molecularly imprinted electrochemical transducer. The transducer disclosed by the invention has good selectivity and high flexibility on a biomarker of 3-nitrotyrosine, is simple to manufacture, has stable performance, and can be used repeatedly; the preparation cost is low, a sample is simple to pretreat, and the detection is quick; equipment is portable and suitable for site detection.

Description

technical field [0001] The invention belongs to the field of chemical analysis and relates to an electrochemical sensor, in particular to a molecularly imprinted electrochemical sensor, in particular to the preparation and application of a molecularly imprinted electrochemical sensor of a functionalized nano-material of 3-nitrotyrosine. Background technique [0002] The redox system produces a large number of free radicals under unbalanced conditions, and 3-nitrotyrosine (3-NT) is produced by peroxynitrite anion (ONOO) in free radicals. - ) interacts with free tyrosine or tyrosine in the protein structure and is generated by nitration. 3-Nitrotyrosine can change the results and functions of proteins, eventually leading to cell damage. For example, 3-nitrotyrosine in the pancreas can not only cause damage to islet β cells, but also lead to changes in the spatial structure of insulin, thereby reducing the ability of insulin to bind to receptors. [0003] In recent years, for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 潘育方汪世桥翟海云周清杨帆
Owner 康正检测服务股份有限公司
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